Annotations: 1DIN

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1dina_	Alpha and beta proteins (a/b)	alpha/beta-Hydrolases	alpha/beta-Hydrolases	Dienelactone hydrolase	Dienelactone hydrolase	(Pseudomonas knackmussii ) [TaxId: 65741 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Dienelactone hydrolase	8020149	4000714	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	alpha/beta-Hydrolases	8032529	3000102	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Hydrolase_4	e1dinA1	A: a/b three-layered sandwiches	X: alpha/beta-Hydrolases (From Topology)	H: alpha/beta-Hydrolases (From Topology)	T: alpha/beta-Hydrolases	F: Hydrolase_4	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.1820	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Alpha/Beta hydrolase fold, catalytic domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF01738	Dienelactone hydrolase family (DLH)	Dienelactone hydrolase family	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	DIENELACTONE HYDROLASE	carboxymethylenebutenolidase activity hydrolase activity, acting on ester bonds carboxylic ester hydrolase activity hydrolase activity catalytic activity	catabolic process metabolic process cellular process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR051049	Dienelactone hydrolase-like enzyme	Family
A	IPR002925	Dienelactone hydrolase	Domain
A	IPR029058	Alpha/Beta hydrolase fold	Homologous Superfamily

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
A	CSD	Parent Component: CYS RESID: AA0262 PSI-MOD : L-cysteine sulfinic acid MOD:00267

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	carboxymethylenebutenolidase M-CSA #492	Dienelactone hydrolase (DLHase) catalyses the hydrolysis of dienelactone to maleylacetate. This reaction is part of the beta-ketoadipate pathway used in bacteria and fungi to degrade aromatic compounds. Bacteria which utilise this pathway play an important role in the detoxification of many toxic halogenated aromatic compounds produced in industry. DLHase is a member of the alpha/beta hydrolase superfamily, although it does not possess a Ser-Hys-Asp catalytic triad and instead uses a triad of Cys-His-Asp.	Defined by 7 residues: GLU:A-36ILE:A-37TYR:A-85CYS:A-123LEU:A-124 [auth A-123]ASP:A-171 [auth A-170]HIS:A-202 [auth A-201] \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 3.1.1.45 (PDB Primary Data) Search M-CSA Motif + EC 3.1.1.45

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.