Download MendeleycryoEM structure of mTORC1 with the cancer-associated 1455-EWED-1458 duplication variant, overall refinement
Anandapadamanaban, M., Hay, I., Perisic, O., Williams, R.To be published.
8RCH | pdb_00008rch
CryoEM structure of mTORC1 with a paediatric kidney cancer-associated 1455-EWED-1458 duplication in mTOR, overall refinement
- PDB DOI: https://doi.org/10.2210/pdb8RCH/pdb
- EM Map EMD-19052: EMDB EMDataResource
- Classification: SIGNALING PROTEIN
- Organism(s): Homo sapiens
- Expression System: Homo sapiens
- Mutation(s): No
- Deposited: 2023-12-06 Released: 2024-09-11
- Funding Organization(s): Cancer Research UK, Medical Research Council (MRC, United Kingdom), European Molecular Biology Organization (EMBO)
Experimental Data Snapshot
- Method: ELECTRON MICROSCOPY
- Resolution: 4.00 Å
- Aggregation State: PARTICLE
- Reconstruction Method: SINGLE PARTICLE
wwPDB Validation 3D Report Full Report
This is version 1.0 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Serine/threonine-protein kinase mTOR | A, E [auth B] | 2,549 | Homo sapiens | Mutation(s): 0 Gene Names: MTOR, FRAP, FRAP1, FRAP2, RAFT1, RAPT1 EC: 2.7.11.1 (PDB Primary Data), 2.7.10.2 (UniProt) |  |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for P42345 (Homo sapiens) Explore P42345 Go to UniProtKB: P42345 | |||||
PHAROS: P42345 GTEx: ENSG00000198793 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P42345 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Target of rapamycin complex subunit LST8 | B [auth D], F [auth E] | 326 | Homo sapiens | Mutation(s): 0 Gene Names: MLST8, GBL, LST8 |  |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for Q9BVC4 (Homo sapiens) Explore Q9BVC4 Go to UniProtKB: Q9BVC4 | |||||
PHAROS: Q9BVC4 GTEx: ENSG00000167965 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q9BVC4 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 3 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Regulatory-associated protein of mTOR | C [auth W], G [auth Y] | 1,335 | Homo sapiens | Mutation(s): 0 Gene Names: RPTOR, KIAA1303, RAPTOR |  |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for Q8N122 (Homo sapiens) Explore Q8N122 Go to UniProtKB: Q8N122 | |||||
PHAROS: Q8N122 GTEx: ENSG00000141564 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q8N122 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 4 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Eukaryotic translation initiation factor 4E-binding protein 1 | D [auth Z], H [auth X] | 118 | Homo sapiens | Mutation(s): 0 Gene Names: EIF4EBP1 |  |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for Q13541 (Homo sapiens) Explore Q13541 Go to UniProtKB: Q13541 | |||||
PHAROS: Q13541 GTEx: ENSG00000187840 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q13541 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| ANP (Subject of Investigation/LOI) Query on ANP | I [auth A], L [auth B] | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER C10 H17 N6 O12 P3 PVKSNHVPLWYQGJ-KQYNXXCUSA-N |  | ||
| MG (Subject of Investigation/LOI) Query on MG | J [auth A], K [auth A], M [auth B], N [auth B] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: ELECTRON MICROSCOPY
- Resolution: 4.00 Å
- Aggregation State: PARTICLE
- Reconstruction Method: SINGLE PARTICLE
Entry History & Funding Information
Deposition Data
- Released Date: 2024-09-11 Deposition Author(s): Anandapadamanaban, M., Hay, I.M., Perisic, O., Williams, R.L.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Cancer Research UK | United Kingdom | DRCPGM 100014 |
| Medical Research Council (MRC, United Kingdom) | United Kingdom | MC_U105184308 |
| European Molecular Biology Organization (EMBO) | European Union | EMBO ALTF 603-2019 |
Revision History (Full details and data files)
- Version 1.0: 2024-09-11
Type: Initial release
