Download MendeleyPolysaccharide lyase family 33 utilises an evolutionary conserved mechanism of conformationally induced catalysis to drive processivity
Cartmell, A., Couturier, M.To be published.
8R71
Polysaccharide lyase BtPL33HA (BT4410) Y291A with HAdp4 collected at 1.22 A
- PDB DOI: https://doi.org/10.2210/pdb8R71/pdb
- Classification: LYASE
- Organism(s): Bacteroides thetaiotaomicron VPI-5482
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2023-11-23 Released: 2024-12-04
- Funding Organization(s): Royal Society, Wellcome Trust
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.92 Å
- R-Value Free: 0.208
- R-Value Work: 0.179
Starting Model: experimental
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wwPDB Validation 3D Report Full Report
This is version 1.0 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Heparinase | 644 | Bacteroides thetaiotaomicron VPI-5482 | Mutation(s): 1 Gene Names: BT_4410 |  | |
UniProt | |||||
Find proteins for Q89ZG7 (Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)) Explore Q89ZG7 Go to UniProtKB: Q89ZG7 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q89ZG7 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Oligosaccharides
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| P4K Query on P4K | E [auth A], G [auth B] | polyethylene glycol C30 H62 O15 WWPGFZAAWXFBTF-UHFFFAOYSA-N |  | ||
| ZN Query on ZN | F [auth A], H [auth B] | ZINC ION Zn PTFCDOFLOPIGGS-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.92 Å
- R-Value Free: 0.208
- R-Value Work: 0.179
- Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 53.633 | α = 90 |
| b = 137.347 | β = 90 |
| c = 202.832 | γ = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| xia2 | data reduction |
| Aimless | data scaling |
| MOLREP | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2024-12-04 Deposition Author(s): Cartmell, A.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Royal Society | United Kingdom | 212050 |
| Wellcome Trust | United Kingdom | 1065163470 |
Revision History (Full details and data files)
- Version 1.0: 2024-12-04
Type: Initial release
