7PZT | pdb_00007pzt

Structure of the bacterial toxin, TecA, an asparagine deamidase from Alcaligenes faecalis.

PDB DOI: https://doi.org/10.2210/pdb7PZT/pdb

Classification: TOXIN
Organism(s): Alcaligenes faecalis
Expression System: Escherichia coli BL21(DE3)
Mutation(s): No

Deposited: 2021-10-13 Released: 2022-11-02
Deposition Author(s): Dix, S.R., Aziz, A.A., Baker, P.J., Evans, C.A., Dickman, M.J., Farthing, R.J., King, Z.L.S., Nathan, S., Partridge, L.J., Raih, F.M., Sedelnikova, S.E., Thomas, M.S., Rice, D.W.
Funding Organization(s): Royal Society, Biotechnology and Biological Sciences Research Council (BBSRC)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.84 Å
R-Value Free:
0.233 (Depositor), 0.240 (DCC)
R-Value Work:
0.190 (Depositor), 0.200 (DCC)

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

The structure of A. faecalis TecA provides insights into its role as an asparagine deamidase toxin which targets RhoA

Dix, S.R., Aziz, A.A., Baker, B.J., Evans, C.A., Dickman, M.J., Farthing, R.J., King, Z.L.S., Nathan, S., Partridge, L.J., Raih, F.M., Sedelnikova, S.E., Thomas, M.S., Rice, D.W.

To be published.

Macromolecule Content

Total Structure Weight: 35.56 kDa
Atom Count: 2,660
Modelled Residue Count: 320
Deposited Residue Count: 320
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Urea amidohydrolase	A, B	160	Alcaligenes faecalis	Mutation(s): 0 Gene Names: D0C27_02010
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
Sequence Annotations Expand
Reference Sequence LOADING

Small Molecules

Ligands 1 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
MES Query on MES Download Ideal Coordinates CCD File SDF format, chain C [auth B] MOL2 format, chain C [auth B] mmCIF format, chain C [auth B]	C [auth B]	2-(N-MORPHOLINO)-ETHANESULFONIC ACID C₆ H₁₃ N O₄ S SXGZJKUKBWWHRA-UHFFFAOYSA-N		Interactions Focus chain C [auth B] Interactions & Density Focus chain C [auth B]

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.84 Å
R-Value Free: 0.233 (Depositor), 0.240 (DCC)
R-Value Work: 0.190 (Depositor), 0.200 (DCC)

Space Group: P 2₁ 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 47.051	α = 90
b = 71.407	β = 90
c = 95.923	γ = 90

Software Package:

Software Name	Purpose
REFMAC	refinement
DIALS	data reduction
DIALS	data scaling
CRANK2	phasing

Structure Validation

View Full Validation Report

Entry History & Funding Information

Deposition Data

Released Date: 2022-11-02

Deposition Author(s):

Partridge, L.J.

Sedelnikova, S.E.

Funding Organization	Location	Grant Number
Royal Society	United Kingdom	IC170306
Biotechnology and Biological Sciences Research Council (BBSRC)	United Kingdom	BB/M012166/1

Revision History (Full details and data files)

Version 1.0: 2022-11-02
Type: Initial release
Version 1.1: 2024-06-19
Changes: Data collection

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.