7E4V | pdb_00007e4v

Crystal structure of mosquito Staufen dsRNA binding domain 4

PDB DOI: https://doi.org/10.2210/pdb7E4V/pdb

Classification: RNA BINDING PROTEIN
Organism(s): Aedes aegypti
Expression System: Escherichia coli 'BL21-Gold(DE3)pLysS AG
Mutation(s): No

Deposited: 2021-02-15 Released: 2022-02-16
Deposition Author(s): Gayathiri, S.K., Jobichen, C., Mok, Y.K.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.37 Å
R-Value Free:
0.213 (Depositor), 0.210 (DCC)
R-Value Work:
0.192 (Depositor), 0.190 (DCC)
R-Value Observed:
0.193 (Depositor)

Starting Model: experimental
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wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

Crystal structure of mosquito Staufen dsRNA binding domain 4

Gayathiri, S.K., Jobichen, C., Mok, Y.K.

To be published.

Macromolecule Content

Total Structure Weight: 16.73 kDa
Atom Count: 1,353
Modelled Residue Count: 149
Deposited Residue Count: 150
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Staufen	A, B	75	Aedes aegypti	Mutation(s): 0 Gene Names: AAEL007470
UniProt
Find proteins for A0A6I8TL34 (Aedes aegypti) Explore A0A6I8TL34 Go to UniProtKB: A0A6I8TL34
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	A0A6I8TL34
Sequence Annotations Expand
Reference Sequence LOADING

Small Molecules

Ligands 1 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
GOL Query on GOL Download Ideal Coordinates CCD File SDF format, chain C [auth B] MOL2 format, chain C [auth B] mmCIF format, chain C [auth B]	C [auth B]	GLYCEROL C₃ H₈ O₃ PEDCQBHIVMGVHV-UHFFFAOYSA-N		Interactions Focus chain C [auth B] Interactions & Density Focus chain C [auth B]

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.37 Å
R-Value Free: 0.213 (Depositor), 0.210 (DCC)
R-Value Work: 0.192 (Depositor), 0.190 (DCC)
R-Value Observed: 0.193 (Depositor)

Space Group: P 3₂ 2 1

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 42.528	α = 90
b = 42.528	β = 90
c = 155.611	γ = 120

Software Package:

Software Name	Purpose
PHENIX	refinement
PDB_EXTRACT	data extraction
HKL-2000	data reduction
HKL-2000	data scaling
PHASER	phasing

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2022-02-16

Deposition Author(s):

Gayathiri, S.K.

Revision History (Full details and data files)

Version 1.0: 2022-02-16
Type: Initial release
Version 1.1: 2023-11-29
Changes: Data collection, Refinement description

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.