6E0U

Staphylococcus pseudintermedius exfoliative toxin EXI

PDB DOI: https://doi.org/10.2210/pdb6E0U/pdb

Classification: HYDROLASE, TOXIN
Organism(s): Staphylococcus pseudintermedius
Expression System: Escherichia coli BL21(DE3)
Mutation(s): No

Deposited: 2018-07-06 Released: 2019-07-10
Deposition Author(s): Boone, C.D., Laganowsky, A.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.75 Å
R-Value Free:
0.326 (Depositor), 0.320 (DCC)
R-Value Work:
0.284 (Depositor), 0.290 (DCC)
R-Value Observed:
0.286 (Depositor)

Starting Model: experimental
View more details

wwPDB Validation 3D Report Full Report

This is version 1.1 of the entry. See complete history.

Literature

Crystal structure of the Exfoliative toxin Exi from Staphylococcus pseudintermedius

Boone, C.D., Liu, W., Laganowsky, A., Hook, A.

To be published.

Macromolecule Content

Total Structure Weight: 54.35 kDa
Atom Count: 3,432
Modelled Residue Count: 481
Deposited Residue Count: 486
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Serine protease	A, B	243	Staphylococcus pseudintermedius	Mutation(s): 0 Gene Names: exi EC: 3.4.21
UniProt
Find proteins for D0VXY8 (Staphylococcus pseudintermedius) Explore D0VXY8 Go to UniProtKB: D0VXY8
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	D0VXY8
Sequence Annotations Expand
Reference Sequence LOADING

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.75 Å
R-Value Free: 0.326 (Depositor), 0.320 (DCC)
R-Value Work: 0.284 (Depositor), 0.290 (DCC)
R-Value Observed: 0.286 (Depositor)

Space Group: P 2₁ 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 66.23	α = 90
b = 82.13	β = 90
c = 99.56	γ = 90

Software Package:

Software Name	Purpose
XSCALE	data scaling
PHASER	phasing
PHENIX	refinement
PDB_EXTRACT	data extraction
XDS	data reduction

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2019-07-10

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2019-07-10
Type: Initial release
Version 1.1: 2023-10-11
Changes: Data collection, Database references, Refinement description

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.