Download MendeleyCrystal Structure of Human Lactose Synthase forms a Novel 1:2 Complex between beta1,4Galactosyltransferase 1 and alpha-Lactalbumin Proteins
Ramakrishnan, B., Qasba, P.K.To be published.
4L41
Human Lactose synthase: A 2:1 complex between human alpha-lactalbumin and human beta1,4-galactosyltransferase
- PDB DOI: https://doi.org/10.2210/pdb4L41/pdb
- Classification: Calcium Binding Protein/Transferase
- Organism(s): Homo sapiens
- Expression System: Escherichia coli BL21
- Mutation(s): Yes
- Deposited: 2013-06-07 Released: 2013-10-02
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.70 Å
- R-Value Free: 0.237
- R-Value Work: 0.188
- R-Value Observed: 0.190
Starting Models: experimental
View more details
wwPDB Validation 3D Report Full Report
This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Alpha-lactalbumin | A, C [auth B] | 124 | Homo sapiens | Mutation(s): 0 Gene Names: 4-galactosyltransferase, beta1, LALBA, LYZL7 |  |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for P00709 (Homo sapiens) Explore P00709 Go to UniProtKB: P00709 | |||||
PHAROS: P00709 GTEx: ENSG00000167531 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P00709 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Beta-1,4-galactosyltransferase 1 | B [auth C] | 287 | Homo sapiens | Mutation(s): 2 Gene Names: alpha-lactalbumin, B4GALT1, GGTB2 EC: 2.4.1.22 (PDB Primary Data), 2.4.1 (UniProt), 2.4.1.275 (UniProt), 2.4.1.38 (UniProt), 2.4.1.90 (UniProt) |  |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for P15291 (Homo sapiens) Explore P15291 Go to UniProtKB: P15291 | |||||
PHAROS: P15291 GTEx: ENSG00000086062 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P15291 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| SO4 Query on SO4 | E [auth C] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
| CA Query on CA | D [auth A], F [auth B] | CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.70 Å
- R-Value Free: 0.237
- R-Value Work: 0.188
- R-Value Observed: 0.190
- Space Group: P 1 21 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 62.39 | α = 90 |
| b = 65.21 | β = 114.45 |
| c = 71.72 | γ = 90 |
| Software Name | Purpose |
|---|---|
| HKL-2000 | data collection |
| PHENIX | model building |
| PHENIX | refinement |
| HKL-2000 | data reduction |
| HKL-2000 | data scaling |
| PHENIX | phasing |
Entry History
Deposition Data
- Released Date: 2013-10-02 Deposition Author(s): Ramakrishnan, B., Qasba, P.K.
Revision History (Full details and data files)
- Version 1.0: 2013-10-02
Type: Initial release - Version 1.1: 2023-09-20
Changes: Data collection, Database references, Derived calculations, Refinement description - Version 1.2: 2024-11-06
Changes: Structure summary
