1UAX | pdb_00001uax

Crystal structure of the ribonuclease H2 from Pyrococcus horikoshii OT3

PDB DOI: https://doi.org/10.2210/pdb1UAX/pdb

Classification: HYDROLASE
Organism(s): Pyrococcus horikoshii OT3
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2003-03-24 Released: 2004-06-29
Deposition Author(s): Hata, T., Numata, T., Kakuta, Y., Kimura, M.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 2.00 Å
R-Value Free:
0.298 (Depositor), 0.310 (DCC)
R-Value Work:
0.255 (Depositor), 0.260 (DCC)
R-Value Observed:
0.255 (Depositor)

Starting Model: experimental
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wwPDB Validation 3D Report Full Report

This is version 1.3 of the entry. See complete history.

Literature

Crystal structure of the ribonuclease H2 from Pyrococcus horikoshii OT3

Hata, T., Numata, T., Kakuta, Y., Kimura, M.

To be published.

Macromolecule Content

Total Structure Weight: 50.65 kDa
Atom Count: 3,571
Modelled Residue Count: 422
Deposited Residue Count: 440
Unique protein chains: 1

Macromolecules

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(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Ribonuclease HII	A, B	220	Pyrococcus horikoshii OT3	Mutation(s): 0 EC: 3.1.26.4
UniProt
Find proteins for O59351 (Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)) Explore O59351 Go to UniProtKB: O59351
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	O59351
Sequence Annotations Expand
Reference Sequence LOADING

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 2.00 Å
R-Value Free: 0.298 (Depositor), 0.310 (DCC)
R-Value Work: 0.255 (Depositor), 0.260 (DCC)
R-Value Observed: 0.255 (Depositor)

Space Group: P 2₁ 2₁ 2₁

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 27.72	α = 90
b = 97.65	β = 90
c = 159.67	γ = 90

Software Package:

Software Name	Purpose
CNS	refinement
OXFORD	data reduction
d*TREK	data scaling
CNS	phasing

Structure Validation

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Entry History

Deposition Data

Released Date: 2004-06-29

Deposition Author(s):

Revision History (Full details and data files)

Version 1.0: 2004-06-29
Type: Initial release
Version 1.1: 2008-04-27
Changes: Version format compliance
Version 1.2: 2011-07-13
Changes: Source and taxonomy, Version format compliance
Version 1.3: 2023-10-25
Changes: Data collection, Database references, Refinement description

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.