Download MendeleyStructural investigation of Mannanase 26A from Pseudomonas cellulosa reveals an induced fit mechanism and a non-substrate ligand binding site
Oakley, A.J., Wilce, M.C.J.To be published.
1R7O | pdb_00001r7o
Crystal Structure of apo-mannanase 26A from Psudomonas cellulosa
- PDB DOI: https://doi.org/10.2210/pdb1R7O/pdb
- Classification: HYDROLASE
- Organism(s): Cellvibrio japonicus
- Expression System: Escherichia coli BL21
- Mutation(s): No
- Deposited: 2003-10-22 Released: 2003-11-11
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.85 Å
- R-Value Free: 0.170 (Depositor)
- R-Value Work: 0.145 (Depositor), 0.160 (DCC)
- R-Value Observed: 0.146 (Depositor)
Starting Model: experimental
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wwPDB Validation 3D Report Full Report
This is version 1.3 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| mannanase A | 395 | Cellvibrio japonicus | Mutation(s): 0 Gene Names: Man26A EC: 3.2.1.78 |  | |
UniProt | |||||
Find proteins for P49424 (Cellvibrio japonicus (strain Ueda107)) Explore P49424 Go to UniProtKB: P49424 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P49424 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| MES Query on MES | G [auth A] | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID C6 H13 N O4 S SXGZJKUKBWWHRA-UHFFFAOYSA-N |  | ||
| SO4 Query on SO4 | F [auth A] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
| ZN Query on ZN | B [auth A], C [auth A], D [auth A], E [auth A] | ZINC ION Zn PTFCDOFLOPIGGS-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.85 Å
- R-Value Free: 0.170 (Depositor)
- R-Value Work: 0.145 (Depositor), 0.160 (DCC)
- R-Value Observed: 0.146 (Depositor)
Space Group: P 41
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 93.24 | α = 90 |
| b = 93.24 | β = 90 |
| c = 54.83 | γ = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| DENZO | data reduction |
| SCALEPACK | data scaling |
Entry History
Deposition Data
- Released Date: 2003-11-11 Deposition Author(s): Oakley, A.J., Wilce, M.C.J.
Revision History (Full details and data files)
- Version 1.0: 2003-11-11
Type: Initial release - Version 1.1: 2008-04-29
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Version format compliance - Version 1.3: 2023-10-25
Changes: Data collection, Database references, Derived calculations, Refinement description
