Download MendeleyStructure of a Copper-Mediated Base Pair in DNA
Atwell, S., Meggers, E., Spraggon, G., Schultz, P.G.(2001) J Am Chem Soc 123: 12364-12367
- PubMed: 11734038
- DOI: https://doi.org/10.1021/ja011822e
- Primary Citation of Related Structures:
1JES - PubMed Abstract:
Stable and selective DNA base pairing by metal coordination was recently demonstrated with nucleotides containing complementary pyridine-2,6-dicarboxylate (Dipic) and pyridine (Py) bases (Meggers, E.; Holland, P. L.; Tolman; W. B.; Romesberg, F. E.; Schultz, P. G. J. Am. Chem. Soc. 2000, 122, 10714-10715). To understand the structural consequences of introducing this novel base pair into DNA we have solved the crystal structure of a duplex containing the metallo-base pair. The structure shows that the bases pair as designed, but in a Z-DNA conformation. The structure also provides a structural explanation for the B- to Z-DNA transition in this duplex. Further solution studies demonstrate that the metallo-base pair is compatible with Z- or B-DNA conformations, depending on the duplex sequence.
Organizational Affiliation:
Department of Chemistry, The Scripps Research Institute, La Jolla, California 92037, USA.
