1HTL | pdb_00001htl

MUTATION OF A BURIED RESIDUE CAUSES LACK OF ACTIVITY BUT NO CONFORMATIONAL CHANGE: CRYSTAL STRUCTURE OF E. COLI HEAT-LABILE ENTEROTOXIN MUTANT VAL 97--> LYS

Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.50 Å
  • R-Value Work: 
    0.205 (Depositor), 0.190 (DCC) 
  • R-Value Observed: 
    0.205 (Depositor) 

wwPDB Validation   3D Report Full Report


This is version 1.4 of the entry. See complete history. 


Literature

Mutation of a buried residue causes loss of activity but no conformational change in the heat-labile enterotoxin of Escherichia coli.

Merritt, E.A., Sarfaty, S., Pizza, M., Domenighini, M., Rappuoli, R., Hol, W.G.

(1995) Nat Struct Biol 2: 269-272


Macromolecules
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Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
HEAT-LABILE ENTEROTOXIN, SUBUNIT BA [auth D],
B [auth E],
C [auth F],
D [auth G],
E [auth H]		103Escherichia coliMutation(s): 0 
UniProt
Find proteins for P32890 (Escherichia coli)
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UniProt GroupP32890
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  • Reference Sequence
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Entity ID: 2
MoleculeChains Sequence LengthOrganismDetailsImage
HEAT-LABILE ENTEROTOXIN, SUBUNIT AF [auth A]191Escherichia coliMutation(s): 0 
UniProt
Find proteins for P06717 (Escherichia coli)
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Entity ID: 3
MoleculeChains Sequence LengthOrganismDetailsImage
HEAT-LABILE ENTEROTOXIN, SUBUNIT AG [auth C]49Escherichia coliMutation(s): 0 
UniProt
Find proteins for P06717 (Escherichia coli)
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UniProt GroupP06717
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.50 Å
  • R-Value Work:  0.205 (Depositor), 0.190 (DCC) 
  • R-Value Observed: 0.205 (Depositor) 
Space Group: P 21 21 21
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 118.8α = 90
b = 98β = 90
c = 64.8γ = 90
Software Package:
Software NamePurpose
X-PLORmodel building
X-PLORrefinement
X-PLORphasing

Structure Validation

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Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 1995-04-20
    Type: Initial release
  • Version 1.1: 2008-03-21
    Changes: Version format compliance
  • Version 1.2: 2011-07-13
    Changes: Version format compliance
  • Version 1.3: 2024-06-05
    Changes: Data collection, Database references, Other
  • Version 1.4: 2024-10-23
    Changes: Structure summary