2O4E | pdb_00002o4e

The solution structure of a protein-protein interaction module from a family 84 glycoside hydrolase of Clostridium perfringens

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	3D c13-edited noesy-hsqc	1mM U-15N,13C x82, 25mM hepes, 50mM nacl, 5mM cacl2, pH 7.0			7.0	1 atm	298
2	hcch-tocsy	1mM U-15N,13C x82, 25mM hepes, 50mM nacl, 5mM cacl2, pH 7.0			7.0	1 atm	298
3	3D HNCACB	1mM U-15N,13C x82, 25mM hepes, 50mM nacl, 5mM cacl2, pH 7.0			7.0	1 atm	298
4	3D CBCA(CO)NH	1mM U-15N,13C x82, 25mM hepes, 50mM nacl, 5mM cacl2, pH 7.0			7.0	1 atm	298
5	3D C(CO)NH	1mM U-15N,13C x82, 25mM hepes, 50mM nacl, 5mM cacl2, pH 7.0			7.0	1 atm	298
6	3D H(CCO)NH	1mM U-15N,13C x82, 25mM hepes, 50mM nacl, 5mM cacl2, pH 7.0			7.0	1 atm	298
7	3D n15-edited noesy-hsqc	1mM U-15N,13C x82, 25mM hepes, 50mM nacl, 5mM cacl2, pH 7.0			7.0	1 atm	298
8	3D HNHA	1mM U-15N,13C x82, 25mM hepes, 50mM nacl, 5mM cacl2, pH 7.0			7.0	1 atm	298

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Varian	INOVA	800
2	Varian	INOVA	600

NMR Ensemble Information
Conformer Selection Criteria	structures with the lowest energy
Conformers Calculated Total Number	200
Conformers Submitted Total Number	20
Representative Model	1 (lowest energy)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	processing	NMRPipe		Delaglio, F. et al.
2	data analysis	NMRView		Johnson, B.A. et al.
3	refinement	CNS		Brunger, A.T. et al.

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.