2IC4 | pdb_00002ic4

Solution structure of the His402 allotype of the Factor H SCR6-SCR7-SCR8 fragment

SOLUTION SCATTERING

Solution Scattering Data Acquistion	1
Scattering Type	x-ray
Radiation/Neutron Source	ESRF GRENOBLE
Synchrotron	Y
Beamline Type	IDO2
Detector Type	FRELON CCD CAMERA
Detector Manufacturer Details
Temperature (K)	288
pH	7.3
Numer of Time Frames Used	10
Protein Concentration Range (mg/mL)	0.38-0.55
Sample Buffer	9.6 MM NA, K PHOSPHATE 137 MM NACL 3 MM KCL 8 MM NA2HPO4 1 MM KH2PO4
Data Reduction Software	MULTICCD
Guiner Mean Radius Of Gyration (nm)	3.12
Sigma Mean Radius Of Gyration	0.16
R(XS-1) Mean Cross Sectional Radii (nm)	1.04
R(XS-1) Sigma Mean Cross Sectional Radii	0.06
R(XS-2) Mean Cross Sectional Radii (nm)
R(XS-2) Sigma Mean Cross Sectional Radii
P(R) Protein Length (nm)	0.5

Solution Scattering Data Analysis and Model Fitting
Method	Software	Software Authors	Starting Model	Conformers, Number Calculated	Conformers, Number Submitted	Conformers, Selection Criteria	Best Representative Conformer	Other Details
	INSIGHT II, SCTPL7, GNOM				1			THE INFORMATION FOR THE HIS402 ALLOTYPE IS LISTED FIRST IN REMARK 265. THE INFORMATION FOR THE TYR402 ALLOTYPE IS LISTED SECOND. THE COORDINATES CON ...	THE INFORMATION FOR THE HIS402 ALLOTYPE IS LISTED FIRST IN REMARK 265. THE INFORMATION FOR THE TYR402 ALLOTYPE IS LISTED SECOND. THE COORDINATES CONTAIN ONLY CA ATOMS. THE SECOND SET OF RADII: MEAN RADIUS OF GYRATION IS 3.26 NM WITH SIGMA MEAN 0.20. R MEAN CROSS SECTIONAL RADII IS 1.15 NM WITH SIGMA MEAN 0.13.

Crystallization

Crystalization Experiments
ID	Method	pH	Temperature	Details
1		7.3	288	SOLUTION, pH 7.3, temperature 288K

Diffraction

Radiation Source
ID #	Source	Type	Wavelength List	Synchrotron Site	Beamline
1	SYNCHROTRON	ESRF BEAMLINE ID09	1.0	ESRF	ID09

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.