Solution structure of the primary host recognition region of complement factor H
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 3D-15N-HSQC-NOESY | 10% WATER/90% D2O | 200 mM | 4.0 | 1.0 atm | 310.0 | ||
| 2 | 3D-13C- HSQC-NOESY | 10% WATER/90% D2O | 200 mM | 4.0 | 1.0 atm | 310.0 | ||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | AM | 800 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| RESTRAINED MOLECULAR DYNAMICS | CNS | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | LOWEST OVERALL ENERGY |
| Conformers Calculated Total Number | |
| Conformers Submitted Total Number | 26 |
| Representative Model | 1 (n/a) |
| Additional NMR Experimental Information | |
|---|---|
| Details | THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N-LABELED FACTOR H CCP MODULES 19-20 |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | CNS | BRUNGER,ADAMS,CLORE,DELANO,GROS, GROSSE- KUNSTLEVE,JIANG,KUSZEWSKI,NILGES, PANNU,READ, RICE,SIMONSON,WARREN | |
| 2 | structure solution | ANSIG | ||
