1UMQ | pdb_00001umq

solution structure and DNA binding of the effector domain from the global regulator PrrA(RegA) from R. sphaeroides: Insights into DNA binding specificity


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
113C-15N EDITED NOESY 0.66.01 atm275
2CBCA(CO)NH 0.66.01 atm275
3HNCA 0.66.01 atm275
4HNCACB 0.66.01 atm275
5HNCO 0.66.01 atm275
6HN(CA)CO 0.66.01 atm275
7HCCH 0.66.01 atm275
8CCH TOCSY 0.66.01 atm275
913C 0.66.01 atm275
1015N HSQC 0.66.01 atm275
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerDRX500
2BrukerDRX600
NMR Refinement
MethodDetailsSoftware
DISTANCE AND DIHEDRAL RESTRAINTS (TALOS)REFINEMENT IN EXPLICIT WATER AS DESCRIBED IN THE REFERENCE ABOVEARIA
NMR Ensemble Information
Conformer Selection CriteriaRANDOM
Conformers Calculated Total Number20
Conformers Submitted Total Number5
Representative Model1 (n/a)
Additional NMR Experimental Information
DetailsTHE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N-LABELED PRRA C-TERMINAL DOMAIN
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1refinementARIA1.2LINGE ET AL 2003
2structure solutionARIA1.2