Experiment: 1T50

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	NOESY	0.7mM Unlabled protein, 10 mM sodium phosphate buffer	90% H20, 10% D20	1 mM	6.8	ambient	298
2	TOCSY	0.7mM Unlabled protein, 10 mM sodium phosphate buffer	90% H20, 10% D20	1 mM	6.8	ambient	298
3	DQF-COSY	0.7mM Unlabled protein, 10 mM sodium phosphate buffer	90% H20, 10% D20	1 mM	6.8	ambient	298

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Varian	UNITYPLUS	600

NMR Refinement
Method	Details	Software
SELF-CORRECTING DG/ VTF		NOAH/DIAMOND

NMR Ensemble Information
Conformer Selection Criteria	VTF VALUES
Conformers Calculated Total Number	50
Conformers Submitted Total Number	20
Representative Model	1 (closest to the average)

Additional NMR Experimental Information
Details	HOMONUCLEAR 1H NMR SPECTROSCOPY

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	structure solution	NOAH/DIAMOND
2	refinement	FANTOM

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.