STRUCTURAL ROLE OF A BURIED SALT BRIDGE IN THE 434 REPRESSOR DNA-BINDING DOMAIN, NMR, 20 STRUCTURES
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 15N-RESOLVED NOESY | 4.8 | 286 | |||||
| 2 | HOMONUCLEAR NOESY | 4.8 | 286 | |||||
| 3 | E.COSY | 4.8 | 286 | |||||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | AMX600 | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| DISTANCE GEOMETRY WITH DIANA, ENERGY MINIMIZATION WITH OPAL | OPAL | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | DIANA PENALTY FUNCTION |
| Conformers Calculated Total Number | 20 |
| Conformers Submitted Total Number | 20 |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | OPAL | LUGINBUHL,GUNTERT,BILLETER,WUTHRICH | |
| 2 | structure solution | BRUKER UXNMR | UXNMR | |
| 3 | structure solution | PROSA | ||
| 4 | structure solution | DIANA | ||
| 5 | structure solution | OPAL | ||
