Annotations: 8U7C

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	NEMO IKK-binding domain-like	8102933	3002761	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	NEMO IKK-binding domain-like	8102933	3002761	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	NEMO IKK-binding domain-like	8102933	3002761	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	NEMO IKK-binding domain-like	8102933	3002761	SCOP2B (2022-06-29)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF11577	NF-kappa-B essential modulator NEMO (NEMO)	NF-kappa-B essential modulator NEMO	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	Engineered NEMO minimal IKK-binding domain	protein binding binding protein domain specific binding protein-macromolecule adaptor activity molecular adaptor activity signaling adaptor activity protein heterodimerization activity protein dimerization activity transferrin receptor binding signaling receptor binding ubiquitin binding ubiquitin-like protein binding linear polyubiquitin binding polyubiquitin modification-dependent protein binding protein binding binding protein domain specific binding protein-macromolecule adaptor activity molecular adaptor activity signaling adaptor activity protein heterodimerization activity protein dimerization activity transferrin receptor binding signaling receptor binding ubiquitin binding ubiquitin-like protein binding linear polyubiquitin binding polyubiquitin modification-dependent protein binding modification-dependent protein binding K63-linked polyubiquitin modification-dependent protein binding identical protein binding protein homodimerization activity ubiquitin protein ligase binding ubiquitin-like protein ligase binding enzyme binding cation binding ion binding metal ion binding small molecule binding	cellular response to stimulus cellular response to stress response to stimulus DNA damage response response to stress cellular process cellular component organization cellular component biogenesis cellular component assembly protein-containing complex organization protein-containing complex assembly cellular component organization or biogenesis positive regulation of cell communication regulation of cellular process cellular response to stimulus cellular response to stress response to stimulus DNA damage response response to stress cellular process cellular component organization cellular component biogenesis cellular component assembly protein-containing complex organization protein-containing complex assembly cellular component organization or biogenesis positive regulation of cell communication regulation of cellular process positive regulation of signal transduction positive regulation of canonical NF-kappaB signal transduction signaling positive regulation of biological process regulation of signal transduction intracellular signal transduction cell communication positive regulation of response to stimulus regulation of intracellular signal transduction intracellular signaling cassette signal transduction positive regulation of cellular process regulation of biological process regulation of signaling biological regulation positive regulation of intracellular signal transduction canonical NF-kappaB signal transduction regulation of response to stimulus regulation of cell communication regulation of canonical NF-kappaB signal transduction positive regulation of signaling immune response-regulating cell surface receptor signaling pathway immune response-activating signaling pathway immune response-regulating signaling pathway positive regulation of immune response regulation of immune system process positive regulation of immune system process cell surface receptor signaling pathway immune response-activating cell surface receptor signaling pathway immune response T cell receptor signaling pathway immune system process regulation of immune response antigen receptor-mediated signaling pathway activation of immune response catabolic process positive regulation of protein metabolic process proteolysis involved in protein catabolic process macromolecule catabolic process regulation of ubiquitin-dependent protein catabolic process regulation of primary metabolic process positive regulation of metabolic process positive regulation of ubiquitin-dependent protein catabolic process regulation of proteolysis regulation of proteolysis involved in protein catabolic process modification-dependent macromolecule catabolic process proteolysis positive regulation of macromolecule metabolic process protein metabolic process regulation of protein metabolic process primary metabolic process regulation of macromolecule metabolic process protein catabolic process regulation of catabolic process positive regulation of proteolysis involved in protein catabolic process positive regulation of catabolic process modification-dependent protein catabolic process positive regulation of proteolysis metabolic process ubiquitin-dependent protein catabolic process macromolecule metabolic process regulation of metabolic process apoptotic process cell death programmed cell death biological process involved in interspecies interaction between organisms response to other organism defense response defense response to symbiont response to external stimulus response to biotic stimulus defense response to other organism innate immune response response to external biotic stimulus defense response to bacterium response to bacterium positive regulation of T cell receptor signaling pathway regulation of antigen receptor-mediated signaling pathway regulation of T cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway positive regulation of DNA-binding transcription factor activity positive regulation of NF-kappaB transcription factor activity RNA metabolic process nucleobase-containing compound metabolic process RNA biosynthetic process positive regulation of molecular function nucleic acid biosynthetic process DNA-templated transcription regulation of RNA metabolic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of biosynthetic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of molecular function regulation of gene expression biosynthetic process regulation of DNA-binding transcription factor activity regulation of macromolecule biosynthetic process regulation of DNA-templated transcription positive regulation of autophagy macroautophagy autophagy regulation of autophagy regulation of macroautophagy positive regulation of macroautophagy process utilizing autophagic mechanism response to virus vesicle localization establishment of organelle localization localization cellular localization organelle localization establishment of vesicle localization establishment of localization in cell establishment of localization inflammatory response anoikis positive regulation of DNA-templated transcription positive regulation of macromolecule biosynthetic process transcription by RNA polymerase II positive regulation of biosynthetic process regulation of transcription by RNA polymerase II positive regulation of nucleobase-containing compound metabolic process positive regulation of transcription by RNA polymerase II positive regulation of RNA metabolic process positive regulation of RNA biosynthetic process	intracellular organelle cellular anatomical structure intracellular anatomical structure cytoskeleton spindle organelle membraneless organelle intracellular membraneless organelle spindle pole microtubule cytoskeleton cytoplasm cytosol mitotic spindle membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure cytoskeleton spindle organelle membraneless organelle intracellular membraneless organelle spindle pole microtubule cytoskeleton cytoplasm cytosol mitotic spindle membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen protein-containing complex catalytic complex IkappaB kinase complex transferase complex protein kinase complex transferase complex, transferring phosphorus-containing groups serine/threonine protein kinase complex ubiquitin ligase complex intracellular protein-containing complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR034735	NEMO, Zinc finger	Domain
A, B, C, D	IPR021063	NF-kappa-B essential modulator NEMO, N-terminal	Domain
A, B, C, D	IPR032419	NF-kappa-B essential modulator NEMO, CC2-LZ domain	Domain
A, B, C, D	IPR051301	Optineurin/NF-kappa-B Essential Modulator	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B, C, D	Pharos: Q9Y6K9	incontinentia pigmenti immunodeficiency without anhidrotic ectodermal dysplasia osteopetrosis anhidrotic ectodermal dysplasia-immunodeficiency-osteopetrosis-lymphedema syndrome immunodeficiency 33 non-alcoholic fatty liver disease ectodermal dysplasia and immune deficiency Invasive pneumococcal disease, recurrent isolated, 2 ectodermal dysplasia and immunodeficiency 1 nevus of Ito ectodermal dysplasia syndrome intellectual disability Ito hypomelanosis lymphatic system disease hyper-IgM syndrome type 1 incontinentia pigmenti immunodeficiency without anhidrotic ectodermal dysplasia osteopetrosis anhidrotic ectodermal dysplasia-immunodeficiency-osteopetrosis-lymphedema syndrome immunodeficiency 33 non-alcoholic fatty liver disease ectodermal dysplasia and immune deficiency Invasive pneumococcal disease, recurrent isolated, 2 ectodermal dysplasia and immunodeficiency 1 nevus of Ito ectodermal dysplasia syndrome intellectual disability Ito hypomelanosis lymphatic system disease hyper-IgM syndrome type 1 lung benign neoplasm

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.