8BWM | pdb_00008bwm

Crystal structure of human Twisted gastrulation protein homolog 1 (TWSG1) in complex with human Growth Differentiation factor 5 (GDF5) and calcium, long-wavelength X-ray dataset (4042 eV)

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Cystine-knot cytokines	8070313	3000846	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Cystine-knot cytokines	8070313	3000846	SCOP2B (2022-06-29)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF00019	Transforming growth factor beta like domain (TGF_beta)	Transforming growth factor beta like domain		Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Growth/differentiation factor 5	molecular transducer activity binding molecular function activator activity signaling receptor binding signaling receptor activity growth factor activity signaling receptor activator activity protein binding molecular function regulator activity signaling receptor regulator activity receptor ligand activity BMP binding cytokine binding cytokine activity molecular transducer activity binding molecular function activator activity signaling receptor binding signaling receptor activity growth factor activity signaling receptor activator activity protein binding molecular function regulator activity signaling receptor regulator activity receptor ligand activity BMP binding cytokine binding cytokine activity identical protein binding	cellular response to stimulus positive regulation of cell communication cell surface receptor protein serine/threonine kinase signaling pathway regulation of cellular process positive regulation of signal transduction response to stimulus signaling regulation of transmembrane receptor protein serine/threonine kinase signaling pathway positive regulation of biological process regulation of signal transduction intracellular signal transduction cell communication positive regulation of response to stimulus regulation of intracellular signal transduction intracellular signaling cassette cellular response to stimulus positive regulation of cell communication cell surface receptor protein serine/threonine kinase signaling pathway regulation of cellular process positive regulation of signal transduction response to stimulus signaling regulation of transmembrane receptor protein serine/threonine kinase signaling pathway positive regulation of biological process regulation of signal transduction intracellular signal transduction cell communication positive regulation of response to stimulus regulation of intracellular signal transduction intracellular signaling cassette signal transduction enzyme-linked receptor protein signaling pathway positive regulation of cellular process regulation of biological process regulation of signaling cell surface receptor signaling pathway biological regulation positive regulation of intracellular signal transduction positive regulation of transmembrane receptor protein serine/threonine kinase signaling pathway SMAD protein signal transduction regulation of response to stimulus regulation of cell communication cellular process regulation of SMAD protein signal transduction positive regulation of SMAD protein signal transduction positive regulation of signaling cellular response to BMP stimulus cellular response to growth factor stimulus response to endogenous stimulus cellular response to endogenous stimulus response to BMP transforming growth factor beta receptor superfamily signaling pathway response to growth factor BMP signaling pathway developmental process multicellular organismal process cellular developmental process connective tissue development animal organ development tissue development chondroblast differentiation multicellular organism development skeletal system development cell differentiation anatomical structure development cartilage development system development cell-cell signaling regulation of cartilage development regulation of chondrocyte differentiation regulation of developmental process regulation of cell differentiation positive regulation of chondrocyte differentiation positive regulation of developmental process positive regulation of cell differentiation regulation of multicellular organismal process regulation of multicellular organismal development chondrocyte differentiation positive regulation of multicellular organismal process positive regulation of cartilage development neuron apoptotic process negative regulation of neuron apoptotic process negative regulation of programmed cell death regulation of apoptotic process negative regulation of biological process regulation of programmed cell death cell death negative regulation of apoptotic process negative regulation of cellular process apoptotic process programmed cell death regulation of neuron apoptotic process limb development appendage development embryonic limb morphogenesis embryonic morphogenesis embryonic appendage morphogenesis embryo development anatomical structure morphogenesis appendage morphogenesis limb morphogenesis regulation of multicellular organism growth regulation of growth regulation of developmental growth developmental growth growth multicellular organism growth ossification tissue remodeling bone remodeling ossification involved in bone remodeling positive regulation of BMP signaling pathway regulation of BMP signaling pathway regulation of cellular response to growth factor stimulus response to mechanical stimulus response to abiotic stimulus response to external stimulus positive regulation of neuron differentiation neurogenesis neuron differentiation nervous system development generation of neurons regulation of neuron differentiation negative regulation of mesenchymal cell apoptotic process mesenchymal cell apoptotic process regulation of mesenchymal cell apoptotic process forelimb morphogenesis negative regulation of cartilage development negative regulation of chondrocyte differentiation negative regulation of cell differentiation negative regulation of developmental process negative regulation of multicellular organismal process negative regulation of cell population proliferation cell population proliferation regulation of cell population proliferation regulation of epithelial cell proliferation epithelial cell proliferation negative regulation of epithelial cell proliferation hindlimb morphogenesis cellular response to transforming growth factor beta stimulus response to transforming growth factor beta transforming growth factor beta receptor signaling pathway	cell periphery cellular anatomical structure plasma membrane membrane extracellular region extracellular space
C, D	Twisted gastrulation protein homolog 1	transforming growth factor beta binding protein binding binding growth factor binding cytokine binding heparin binding glycosaminoglycan binding carbohydrate derivative binding sulfur compound binding	multicellular organismal process regulation of cellular process regulation of alpha-beta T cell activation regulation of leukocyte activation negative regulation of biological process regulation of cell activation lymphocyte activation negative regulation of lymphocyte activation leukocyte cell-cell adhesion negative regulation of cell adhesion leukocyte activation cell adhesion cell-cell adhesion T cell activation CD4-positive, alpha-beta T cell activation multicellular organismal process regulation of cellular process regulation of alpha-beta T cell activation regulation of leukocyte activation negative regulation of biological process regulation of cell activation lymphocyte activation negative regulation of lymphocyte activation leukocyte cell-cell adhesion negative regulation of cell adhesion leukocyte activation cell adhesion cell-cell adhesion T cell activation CD4-positive, alpha-beta T cell activation negative regulation of cellular process alpha-beta T cell activation regulation of immune system process regulation of multicellular organismal process regulation of cell-cell adhesion regulation of biological process regulation of cell adhesion cell activation biological regulation regulation of T cell activation negative regulation of cell activation regulation of CD4-positive, alpha-beta T cell activation negative regulation of CD4-positive, alpha-beta T cell activation negative regulation of T cell activation negative regulation of immune system process negative regulation of leukocyte cell-cell adhesion immune system process negative regulation of cell-cell adhesion regulation of leukocyte cell-cell adhesion cellular process negative regulation of alpha-beta T cell activation negative regulation of multicellular organismal process negative regulation of leukocyte activation regulation of lymphocyte activation cellular response to stimulus positive regulation of cell communication cell surface receptor protein serine/threonine kinase signaling pathway positive regulation of signal transduction cellular response to growth factor stimulus response to stimulus signaling regulation of transmembrane receptor protein serine/threonine kinase signaling pathway positive regulation of biological process regulation of signal transduction positive regulation of BMP signaling pathway cell communication positive regulation of response to stimulus cellular response to endogenous stimulus signal transduction BMP signaling pathway enzyme-linked receptor protein signaling pathway positive regulation of cellular process cellular response to BMP stimulus regulation of signaling cell surface receptor signaling pathway regulation of BMP signaling pathway response to endogenous stimulus regulation of cellular response to growth factor stimulus positive regulation of transmembrane receptor protein serine/threonine kinase signaling pathway response to BMP regulation of response to stimulus regulation of cell communication transforming growth factor beta receptor superfamily signaling pathway response to growth factor positive regulation of signaling intracellular signal transduction regulation of intracellular signal transduction intracellular signaling cassette positive regulation of intracellular signal transduction SMAD protein signal transduction regulation of SMAD protein signal transduction positive regulation of SMAD protein signal transduction cytokine production regulation of cytokine production negative regulation of macromolecule metabolic process regulation of biosynthetic process negative regulation of metabolic process negative regulation of cytokine production regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process regulation of gene expression negative regulation of gene expression biosynthetic process negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process regulation of macromolecule biosynthetic process metabolic process macromolecule metabolic process regulation of metabolic process developmental process gland development animal organ development salivary gland development anatomical structure morphogenesis multicellular organism development gland morphogenesis salivary gland morphogenesis anatomical structure development animal organ morphogenesis exocrine system development system development head development forebrain development brain development nervous system development central nervous system development sensory organ development eye development visual system development camera-type eye development sensory system development ossification cellular response to transforming growth factor beta stimulus regulation of transforming growth factor beta receptor signaling pathway regulation of cellular response to transforming growth factor beta stimulus positive regulation of transforming growth factor beta receptor signaling pathway positive regulation of cellular response to transforming growth factor beta stimulus response to transforming growth factor beta transforming growth factor beta receptor signaling pathway regulation of osteoblast differentiation regulation of developmental process cellular developmental process negative regulation of osteoblast differentiation negative regulation of developmental process regulation of cell differentiation osteoblast differentiation cell differentiation negative regulation of cell differentiation connective tissue development tissue development skeletal system development chondrocyte differentiation cartilage development negative regulation of lymphocyte proliferation negative regulation of T cell proliferation negative regulation of leukocyte proliferation alpha-beta T cell proliferation lymphocyte proliferation regulation of leukocyte proliferation cell population proliferation regulation of CD4-positive, alpha-beta T cell proliferation mononuclear cell proliferation regulation of cell population proliferation leukocyte proliferation negative regulation of mononuclear cell proliferation regulation of alpha-beta T cell proliferation T cell proliferation regulation of lymphocyte proliferation CD4-positive, alpha-beta T cell proliferation negative regulation of cell population proliferation regulation of T cell proliferation negative regulation of alpha-beta T cell proliferation negative regulation of CD4-positive, alpha-beta T cell proliferation regulation of mononuclear cell proliferation hemopoiesis cell development negative regulation of signaling negative regulation of cellular response to growth factor stimulus negative regulation of BMP signaling pathway negative regulation of cell communication negative regulation of response to stimulus negative regulation of signal transduction negative regulation of transmembrane receptor protein serine/threonine kinase signaling pathway formation of primary germ layer mesoderm morphogenesis embryonic morphogenesis embryo development gastrulation tissue morphogenesis anatomical structure formation involved in morphogenesis mesoderm formation mesoderm development	cellular anatomical structure extracellular region extracellular space

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR029034	Cystine-knot cytokine	Homologous Superfamily
A, B	IPR015615	Transforming growth factor-beta-related	Family
A, B	IPR017948	Transforming growth factor beta, conserved site	Conserved Site
A, B	IPR001111	TGF-beta, propeptide	Domain
A, B	IPR001839	Transforming growth factor-beta, C-terminal	Domain
C, D	IPR006761	Twisted gastrulation (Tsg) protein	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B	Pharos: P43026	fibular hypoplasia and complex brachydactyly multiple synostoses syndrome 2 acromesomelic dysplasia, Hunter-Thompson type osteoarthritis symphalangism, proximal, 1B acromesomelic dysplasia, Grebe type brachydactyly type C proximal symphalangism brachydactyly type A1C brachydactyly type A2 rheumatoid arthritis developmental dysplasia of the hip 1 calcaneonavicular coalition brachydactyly Hip Dislocation, Congenital fibular hypoplasia and complex brachydactyly multiple synostoses syndrome 2 acromesomelic dysplasia, Hunter-Thompson type osteoarthritis symphalangism, proximal, 1B acromesomelic dysplasia, Grebe type brachydactyly type C proximal symphalangism brachydactyly type A1C brachydactyly type A2 rheumatoid arthritis developmental dysplasia of the hip 1 calcaneonavicular coalition brachydactyly Hip Dislocation, Congenital malignant mesothelioma osteoarthritis susceptibility 5 acromesomelic dysplasia osteogenesis imperfecta atypical teratoid rhabdoid tumor skin carcinoma intellectual disability multiple synostoses syndrome osteoarthritis, knee osteosarcoma proximal symphalangism 1A bone development disease brachydactyly type A1 large cell medulloblastoma multiple synostoses syndrome 1 osteoarthritis susceptibility 1 polydactyly angel-shaped phalango-epiphyseal dysplasia brachydactyly type A1A
C, D	Pharos: Q9GZX9	holoprosencephaly Jaw Abnormalities alobar holoprosencephaly breast cancer malignant ependymoma osteosarcoma pilocytic astrocytoma ulcerative colitis Crohn disease malignant mesothelioma ovarian cancer progressive supranuclear palsy atypical teratoid rhabdoid tumor glioblastoma lobar holoprosencephaly holoprosencephaly Jaw Abnormalities alobar holoprosencephaly breast cancer malignant ependymoma osteosarcoma pilocytic astrocytoma ulcerative colitis Crohn disease malignant mesothelioma ovarian cancer progressive supranuclear palsy atypical teratoid rhabdoid tumor glioblastoma lobar holoprosencephaly nasopharyngeal carcinoma pancreatic ductal adenocarcinoma semilobar holoprosencephaly

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.