Annotations: 8A0E

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	DHS-like NAD/FAD-binding domain	8044242	3001728	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	DHS-like NAD/FAD-binding domain	8044242	3001728	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	DHS-like NAD/FAD-binding domain	8044242	3001728	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	DHS-like NAD/FAD-binding domain	8044242	3001728	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	F_UNCLASSIFIED	e8a0eA1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: DHS-like NAD/FAD-binding domain	F: F_UNCLASSIFIED	ECOD (1.6)
B	F_UNCLASSIFIED	e8a0eB1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: DHS-like NAD/FAD-binding domain	F: F_UNCLASSIFIED	ECOD (1.6)
D	F_UNCLASSIFIED	e8a0eD1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: DHS-like NAD/FAD-binding domain	F: F_UNCLASSIFIED	ECOD (1.6)
C	F_UNCLASSIFIED	e8a0eC1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: DHS-like NAD/FAD-binding domain	F: F_UNCLASSIFIED	ECOD (1.6)
E	F_UNCLASSIFIED	e8a0eE1	A: beta barrels	X: OB-fold	H: Nucleic acid-binding proteins (From Topology)	T: Nucleic acid-binding proteins	F: F_UNCLASSIFIED	ECOD (1.6)
E	F_UNCLASSIFIED	e8a0eE2	A: beta barrels	X: SH3	H: SH3	T: SH3	F: F_UNCLASSIFIED	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF01916	Deoxyhypusine synthase (DS)	Deoxyhypusine synthase	Eukaryotic initiation factor 5A (eIF-5A) contains an unusual amino acid, hypusine [N epsilon-(4-aminobutyl-2-hydroxy)lysine]. The first step in the post-translational formation of hypusine is catalysed by the enzyme deoxyhypusine synthase (DS) EC:1.1 ...	Eukaryotic initiation factor 5A (eIF-5A) contains an unusual amino acid, hypusine [N epsilon-(4-aminobutyl-2-hydroxy)lysine]. The first step in the post-translational formation of hypusine is catalysed by the enzyme deoxyhypusine synthase (DS) EC:1.1.1.249. The modified version of eIF-5A, and DS, are required for eukaryotic cell proliferation [1].	Domain
C, D	PF01916	Deoxyhypusine synthase (DS)	Deoxyhypusine synthase	Eukaryotic initiation factor 5A (eIF-5A) contains an unusual amino acid, hypusine [N epsilon-(4-aminobutyl-2-hydroxy)lysine]. The first step in the post-translational formation of hypusine is catalysed by the enzyme deoxyhypusine synthase (DS) EC:1.1 ...	Eukaryotic initiation factor 5A (eIF-5A) contains an unusual amino acid, hypusine [N epsilon-(4-aminobutyl-2-hydroxy)lysine]. The first step in the post-translational formation of hypusine is catalysed by the enzyme deoxyhypusine synthase (DS) EC:1.1.1.249. The modified version of eIF-5A, and DS, are required for eukaryotic cell proliferation [1].	Domain
E	PF21485	Translation initiation factor 5A-like, N-terminal (IF5A-like_N)	Translation initiation factor 5A-like, N-terminal	This is the N-terminal domain of Translation initiation factor 5A from eukaryotes and archaea, which has a SH2-like fold. These proteins are involved in translation elongation and eIF5A is specifically required to promote peptide-bond formation betwe ...	This is the N-terminal domain of Translation initiation factor 5A from eukaryotes and archaea, which has a SH2-like fold. These proteins are involved in translation elongation and eIF5A is specifically required to promote peptide-bond formation between consecutive proline residues [1-5]. This entry also includes Woronin body major protein Hex1, which is similar to eIF5A.	Domain
E	PF01287	Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold (eIF-5a)	Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold	eIF5A, previously thought to be an initiation factor, has been shown to be required for peptide chain elongation in yeast [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Deoxyhypusine synthase	deoxyhypusine synthase activity transferase activity transferase activity, transferring alkyl or aryl (other than methyl) groups catalytic activity identical protein binding protein binding binding	regulation of biological process cell population proliferation regulation of cellular process positive regulation of cell population proliferation regulation of cell population proliferation biological regulation positive regulation of biological process cellular process positive regulation of cellular process spermidine catabolic process catabolic process polyamine metabolic process biogenic amine catabolic process amine metabolic process regulation of biological process cell population proliferation regulation of cellular process positive regulation of cell population proliferation regulation of cell population proliferation biological regulation positive regulation of biological process cellular process positive regulation of cellular process spermidine catabolic process catabolic process polyamine metabolic process biogenic amine catabolic process amine metabolic process spermidine metabolic process metabolic process polyamine catabolic process biogenic amine metabolic process amine catabolic process peptidyl-lysine modification to peptidyl-hypusine peptidyl-lysine modification primary metabolic process gene expression macromolecule biosynthetic process protein maturation biosynthetic process macromolecule modification peptidyl-amino acid modification protein metabolic process macromolecule metabolic process protein modification process multicellular organismal process regulation of leukocyte activation regulation of cell activation positive regulation of cell activation T cell activation lymphocyte proliferation positive regulation of leukocyte activation regulation of immune system process positive regulation of immune system process regulation of leukocyte proliferation positive regulation of T cell activation positive regulation of lymphocyte proliferation positive regulation of T cell proliferation mononuclear cell proliferation regulation of leukocyte cell-cell adhesion positive regulation of leukocyte proliferation positive regulation of multicellular organismal process positive regulation of leukocyte cell-cell adhesion lymphocyte activation leukocyte proliferation leukocyte cell-cell adhesion leukocyte activation positive regulation of cell-cell adhesion positive regulation of lymphocyte activation cell adhesion cell-cell adhesion positive regulation of mononuclear cell proliferation regulation of multicellular organismal process regulation of cell-cell adhesion T cell proliferation regulation of lymphocyte proliferation positive regulation of cell adhesion regulation of cell adhesion cell activation regulation of T cell activation regulation of T cell proliferation immune system process regulation of mononuclear cell proliferation regulation of lymphocyte activation chemical homeostasis homeostatic process glucose homeostasis carbohydrate homeostasis translation	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol
C, D	Deoxyhypusine synthase	deoxyhypusine synthase activity transferase activity transferase activity, transferring alkyl or aryl (other than methyl) groups catalytic activity identical protein binding protein binding binding	regulation of biological process cell population proliferation regulation of cellular process positive regulation of cell population proliferation regulation of cell population proliferation biological regulation positive regulation of biological process cellular process positive regulation of cellular process spermidine catabolic process catabolic process polyamine metabolic process biogenic amine catabolic process amine metabolic process regulation of biological process cell population proliferation regulation of cellular process positive regulation of cell population proliferation regulation of cell population proliferation biological regulation positive regulation of biological process cellular process positive regulation of cellular process spermidine catabolic process catabolic process polyamine metabolic process biogenic amine catabolic process amine metabolic process spermidine metabolic process metabolic process polyamine catabolic process biogenic amine metabolic process amine catabolic process peptidyl-lysine modification to peptidyl-hypusine peptidyl-lysine modification primary metabolic process gene expression macromolecule biosynthetic process protein maturation biosynthetic process macromolecule modification peptidyl-amino acid modification protein metabolic process macromolecule metabolic process protein modification process multicellular organismal process regulation of leukocyte activation regulation of cell activation positive regulation of cell activation T cell activation lymphocyte proliferation positive regulation of leukocyte activation regulation of immune system process positive regulation of immune system process regulation of leukocyte proliferation positive regulation of T cell activation positive regulation of lymphocyte proliferation positive regulation of T cell proliferation mononuclear cell proliferation regulation of leukocyte cell-cell adhesion positive regulation of leukocyte proliferation positive regulation of multicellular organismal process positive regulation of leukocyte cell-cell adhesion lymphocyte activation leukocyte proliferation leukocyte cell-cell adhesion leukocyte activation positive regulation of cell-cell adhesion positive regulation of lymphocyte activation cell adhesion cell-cell adhesion positive regulation of mononuclear cell proliferation regulation of multicellular organismal process regulation of cell-cell adhesion T cell proliferation regulation of lymphocyte proliferation positive regulation of cell adhesion regulation of cell adhesion cell activation regulation of T cell activation regulation of T cell proliferation immune system process regulation of mononuclear cell proliferation regulation of lymphocyte activation chemical homeostasis homeostatic process glucose homeostasis carbohydrate homeostasis translation	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol
E	Eukaryotic translation initiation factor 5A	translation regulator activity binding organic cyclic compound binding translation regulator activity, nucleic acid binding translation elongation factor activity nucleic acid binding translation factor activity, RNA binding RNA binding U6 snRNA binding snRNA binding ribonucleoprotein complex binding protein-containing complex binding ribosome binding	regulation of cellular process regulation of cellular component organization positive regulation of cellular component organization positive regulation of protein metabolic process positive regulation of biological process protein-containing complex disassembly positive regulation of translation positive regulation of macromolecule biosynthetic process regulation of protein-containing complex disassembly regulation of primary metabolic process cellular component organization post-transcriptional regulation of gene expression positive regulation of metabolic process positive regulation of biosynthetic process regulation of cellular process regulation of cellular component organization positive regulation of cellular component organization positive regulation of protein metabolic process positive regulation of biological process protein-containing complex disassembly positive regulation of translation positive regulation of macromolecule biosynthetic process regulation of protein-containing complex disassembly regulation of primary metabolic process cellular component organization post-transcriptional regulation of gene expression positive regulation of metabolic process positive regulation of biosynthetic process positive regulation of macromolecule metabolic process translational termination cellular component organization or biogenesis protein metabolic process positive regulation of protein-containing complex disassembly positive regulation of cellular process cellular component disassembly regulation of biosynthetic process regulation of biological process protein-containing complex organization regulation of protein metabolic process biological regulation regulation of translational termination primary metabolic process regulation of macromolecule metabolic process translation gene expression macromolecule biosynthetic process regulation of gene expression biosynthetic process positive regulation of gene expression positive regulation of translational termination regulation of translation cellular process metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of metabolic process cellular response to stimulus positive regulation of cell communication positive regulation of signal transduction response to stimulus signaling positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator regulation of signal transduction regulation of programmed cell death regulation of intrinsic apoptotic signaling pathway intracellular signal transduction cell communication positive regulation of apoptotic signaling pathway positive regulation of response to stimulus regulation of intracellular signal transduction regulation of signal transduction by p53 class mediator positive regulation of signal transduction by p53 class mediator signal transduction intrinsic apoptotic signaling pathway regulation of signaling positive regulation of programmed cell death regulation of apoptotic process positive regulation of intracellular signal transduction signal transduction by p53 class mediator cell death positive regulation of apoptotic process apoptotic signaling pathway regulation of response to stimulus regulation of cell communication intrinsic apoptotic signaling pathway by p53 class mediator positive regulation of intrinsic apoptotic signaling pathway apoptotic process regulation of intrinsic apoptotic signaling pathway by p53 class mediator programmed cell death regulation of apoptotic signaling pathway positive regulation of signaling translational elongation regulation of translational elongation positive regulation of translational elongation cell surface receptor signaling pathway response to cytokine cytokine-mediated signaling pathway response to peptide cellular response to tumor necrosis factor response to chemical tumor necrosis factor-mediated signaling pathway cellular response to cytokine stimulus response to tumor necrosis factor positive regulation of DNA-templated transcription RNA metabolic process nucleobase-containing compound metabolic process RNA biosynthetic process nucleic acid biosynthetic process DNA-templated transcription regulation of RNA metabolic process transcription by RNA polymerase II regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of transcription by RNA polymerase II positive regulation of nucleobase-containing compound metabolic process positive regulation of transcription by RNA polymerase II nucleobase-containing compound biosynthetic process nucleic acid metabolic process positive regulation of RNA metabolic process positive regulation of RNA biosynthetic process regulation of DNA-templated transcription cellular response to virus biological process involved in interspecies interaction between organisms response to other organism response to external stimulus response to external biotic stimulus response to virus response to biotic stimulus	cellular anatomical structure membrane intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle protein-containing complex nucleus nuclear protein-containing complex intracellular membrane-bounded organelle endomembrane system intracellular organelle organelle envelope organelle cellular anatomical structure membrane intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle protein-containing complex nucleus nuclear protein-containing complex intracellular membrane-bounded organelle endomembrane system intracellular organelle organelle envelope organelle nuclear envelope nuclear pore organelle subcompartment endoplasmic reticulum subcompartment nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum organelle membrane endoplasmic reticulum membrane annulate lamellae

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR036982	Deoxyhypusine synthase superfamily	Homologous Superfamily
A, B	IPR029035	DHS-like NAD/FAD-binding domain superfamily	Homologous Superfamily
A, B	IPR002773	Deoxyhypusine synthase	Family
C, D	IPR036982	Deoxyhypusine synthase superfamily	Homologous Superfamily
C, D	IPR029035	DHS-like NAD/FAD-binding domain superfamily	Homologous Superfamily
C, D	IPR002773	Deoxyhypusine synthase	Family
E	IPR014722	Large ribosomal subunit protein uL2, domain 2	Homologous Superfamily
E	IPR020189	Translation elongation factor, IF5A C-terminal	Domain
E	IPR019769	Translation elongation factor, IF5A, hypusine site	PTM
E	IPR001884	Translation elongation factor IF5A-like	Family
E	IPR048670	Translation initiation factor 5A-like, N-terminal	Domain
E	IPR008991	Translation protein SH3-like domain superfamily	Homologous Superfamily
E	IPR012340	Nucleic acid-binding, OB-fold	Homologous Superfamily

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B	Pharos: P49366	acute myeloid leukemia pancreatic ductal adenocarcinoma pulmonary emphysema diabetes mellitus plasma cell myeloma subependymal giant cell astrocytoma osteosarcoma psoriasis
C, D	Pharos: P49366	acute myeloid leukemia pancreatic ductal adenocarcinoma pulmonary emphysema diabetes mellitus plasma cell myeloma subependymal giant cell astrocytoma osteosarcoma psoriasis
E	Pharos: P63241	keloid Mammary Neoplasms, Experimental carcinoma sarcomatoid carcinoma undifferentiated carcinoma Hashimoto thyroiditis psoriasis uterine corpus leiomyoma hypothyroidism mammary neoplasms, animal vascular disease

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
C, D	CSS	Parent Component: CYS RESID: AA0269 PSI-MOD : L-cysteine persulfide MOD:00274

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.