7R3I

PROSS optimitzed variant of RhlR (61 mutations) in complex with the synthetic antagonist mBTL

External Resource: Annotation

Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	GerE	e7r3iA1	A: alpha arrays	X: HTH	H: HTH	T: tetra-helical, LuxR-like	F: GerE	ECOD (1.6)
A	Autoind_bind	e7r3iA2	A: a+b three layers	X: Profilin-like	H: sensor domains (From Topology)	T: sensor domains	F: Autoind_bind	ECOD (1.6)
B	GerE	e7r3iB2	A: alpha arrays	X: HTH	H: HTH	T: tetra-helical, LuxR-like	F: GerE	ECOD (1.6)
B	Autoind_bind	e7r3iB1	A: a+b three layers	X: Profilin-like	H: sensor domains (From Topology)	T: sensor domains	F: Autoind_bind	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF03472	Autoinducer binding domain (Autoind_bind)	Autoinducer binding domain	This domain is found a a large family of transcriptional regulators. This domain specifically binds to autoinducer molecules (N-Acyl homoserine lactones, chlorolactone and rosmarinic acid) (Matilla et. al., FEMS Microbiology Reviews, fuab043, 45, 20 ...	This domain is found a a large family of transcriptional regulators. This domain specifically binds to autoinducer molecules (N-Acyl homoserine lactones, chlorolactone and rosmarinic acid) (Matilla et. al., FEMS Microbiology Reviews, fuab043, 45, 2021, 1. https://doi.org/10.1093/femsre/fuab043).	Domain
A, B	PF00196	Bacterial regulatory proteins, luxR family (GerE)	Bacterial regulatory proteins, luxR family	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	PROSS optimized variant of RhlR with 61 mutations	molecular transducer activity signaling receptor activity DNA binding sequence-specific DNA binding sequence-specific double-stranded DNA binding transcription cis-regulatory region binding binding double-stranded DNA binding organic cyclic compound binding nucleic acid binding transcription regulatory region nucleic acid binding transcription regulator activity DNA-binding transcription factor activity DNA-binding transcription activator activity molecular transducer activity signaling receptor activity DNA binding sequence-specific DNA binding sequence-specific double-stranded DNA binding transcription cis-regulatory region binding binding double-stranded DNA binding organic cyclic compound binding nucleic acid binding transcription regulatory region nucleic acid binding transcription regulator activity DNA-binding transcription factor activity DNA-binding transcription activator activity DNA-binding transcription repressor activity	regulation of biological process regulation of cellular process positive regulation of protein metabolic process regulation of protein metabolic process biological regulation positive regulation of biological process primary metabolic process regulation of macromolecule metabolic process regulation of primary metabolic process positive regulation of metabolic process regulation of proteolysis proteolysis positive regulation of proteolysis metabolic process regulation of biological process regulation of cellular process positive regulation of protein metabolic process regulation of protein metabolic process biological regulation positive regulation of biological process primary metabolic process regulation of macromolecule metabolic process regulation of primary metabolic process positive regulation of metabolic process regulation of proteolysis proteolysis positive regulation of proteolysis metabolic process cellular process positive regulation of macromolecule metabolic process protein metabolic process macromolecule metabolic process regulation of metabolic process positive regulation of cellular process regulation of biosynthetic process pyocyanine biosynthetic process biosynthetic process positive regulation of biosynthetic process positive regulation of pyocyanine biosynthetic process regulation of pyocyanine biosynthetic process gene expression macromolecule biosynthetic process positive regulation of DNA-templated transcription RNA metabolic process positive regulation of macromolecule biosynthetic process nucleobase-containing compound metabolic process RNA biosynthetic process nucleic acid biosynthetic process DNA-templated transcription regulation of RNA metabolic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process positive regulation of nucleobase-containing compound metabolic process nucleobase-containing compound biosynthetic process nucleic acid metabolic process positive regulation of RNA metabolic process regulation of gene expression positive regulation of RNA biosynthetic process regulation of macromolecule biosynthetic process regulation of DNA-templated transcription response to stimulus signaling quorum sensing cell-cell signaling detection of cell density detection of biotic stimulus response to biotic stimulus cell communication detection of stimulus regulation of secondary metabolite biosynthetic process secondary metabolic process positive regulation of secondary metabolite biosynthetic process regulation of secondary metabolic process secondary metabolite biosynthetic process regulation of lipid metabolic process lipid biosynthetic process positive regulation of lipid biosynthetic process positive regulation of lipid metabolic process regulation of lipid biosynthetic process lipid metabolic process	protein-containing complex protein-DNA complex cellular anatomical structure intracellular anatomical structure cytoplasm

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR000792	Transcription regulator LuxR, C-terminal	Domain
A, B	IPR036693	Transcription factor LuxR-like, autoinducer-binding domain superfamily	Homologous Superfamily
A, B	IPR016032	Signal transduction response regulator, C-terminal effector	Homologous Superfamily
A, B	IPR036388	Winged helix-like DNA-binding domain superfamily	Homologous Superfamily
A, B	IPR005143	Transcription factor LuxR-like, autoinducer-binding domain	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.