6O3B

Crystal structure of Frizzled 7 CRD in complex with F6 Fab

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
IMGT Antibody Annotation
SAbDab Antibody Annotation
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d6o3ba2	All beta proteins	Immunoglobulin-like beta-sandwich	Immunoglobulin	C1 set domains (antibody constant domain-like)	automated matches	(Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
A	d6o3ba1	All beta proteins	Immunoglobulin-like beta-sandwich	Immunoglobulin	automated matches	automated matches	(Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
D [auth E]	d6o3be2	All beta proteins	Immunoglobulin-like beta-sandwich	Immunoglobulin	C1 set domains (antibody constant domain-like)	automated matches	(Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
D [auth E]	d6o3be1	All beta proteins	Immunoglobulin-like beta-sandwich	Immunoglobulin	automated matches	automated matches	(Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d6o3bb_	All beta proteins	Immunoglobulin-like beta-sandwich	Immunoglobulin	V set domains (antibody variable domain-like)	automated matches	(Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
E [auth G]	d6o3bg_	All beta proteins	Immunoglobulin-like beta-sandwich	Immunoglobulin	V set domains (antibody variable domain-like)	automated matches	(Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
F [auth H]	d6o3bh1	All alpha proteins	Frizzled cysteine-rich domain	Frizzled cysteine-rich domain	automated matches	automated matches	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
F [auth H]	d6o3bh2	Artifacts	Tags	Tags	Tags	N-terminal Tags	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
C	d6o3bc1	All alpha proteins	Frizzled cysteine-rich domain	Frizzled cysteine-rich domain	automated matches	automated matches	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
C	d6o3bc2	Artifacts	Tags	Tags	Tags	N-terminal Tags	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	C1-set_4	e6o3bA1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: C1-set_4	ECOD (1.6)
A	V-set	e6o3bA2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: V-set	ECOD (1.6)
D [auth E]	C1-set_4	e6o3bE1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: C1-set_4	ECOD (1.6)
D [auth E]	V-set	e6o3bE2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: V-set	ECOD (1.6)
B	V-set_8	e6o3bB2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: V-set_8	ECOD (1.6)
B	C1-set_1	e6o3bB1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: C1-set_1	ECOD (1.6)
E [auth G]	V-set_8	e6o3bG1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: V-set_8	ECOD (1.6)
E [auth G]	C1-set_1	e6o3bG2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: C1-set_1	ECOD (1.6)
F [auth H]	Fz	e6o3bH1	A: few secondary structure elements	X: Frizzled cysteine-rich domain-related (From Topology)	H: Frizzled cysteine-rich domain-related (From Topology)	T: Frizzled cysteine-rich domain-related	F: Fz	ECOD (1.6)
C	Fz	e6o3bC1	A: few secondary structure elements	X: Frizzled cysteine-rich domain-related (From Topology)	H: Frizzled cysteine-rich domain-related (From Topology)	T: Frizzled cysteine-rich domain-related	F: Fz	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (utative)
D [auth E]	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (utative)
B	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (utative)
E [auth G]	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (utative)
F [auth H]	1.10.2000.10	Mainly Alpha	Orthogonal Bundle	Frizzled cysteine-rich domain	Frizzled cysteine-rich domain	CATH (utative)

IMGT Antibody Annotation IMGT Database Homepage

Chain	Protein Name	Description	Organism Name	Gene Allele Name(s)	Domain Name(s)	Receptor Type	Receptor Description	Provenance Source
A, D [auth E]	Fab F2.I	L-KAPPA	Homo sapiens (human)	IGKV1-3901, IGKV1D-3901, IGKJ101, IGKC01	V-DOMAIN V-KAPPA, C-DOMAIN C-KAPPA	IG	FAB-GAMMA-1_KAPPA	IMGT (202449-0)
B, E [auth G]	Fab F2.I	VH-CH1	Homo sapiens (human)	IGHV3-2101, IGHV3-2102, IGHV3-6601, IGHV3-6602, IGHV3-6604, IGHV3-7401, IGHV3-7402, IGHV3-2104, IGHV3-2103, IGHJ401, IGHJ402, IGHJ403, IGHG101, IGHG102, IGHG104, IGHG105, IGHG112, IGHG114, IGHG113, IGHG109, IGHG110, IGHG107, IGHG1*11	V-DOMAIN VH, C-DOMAIN CH1	IG	FAB-GAMMA-1_KAPPA	IMGT (202449-0)
C, F [auth H]	Frizzled-5	FZD8	Homo sapiens (human)	FDZ8*01	R-LIKE-DOMAIN FZD8 (Pr28-158)	FPIA	FZD8	IMGT (202449-0)

SAbDab Antibody Annotation SAbDab Database Homepage

Chain	Chain Class	Chain Subclass	Chain Type	Antigen Name(s)	Provenance Source
A, D [auth E]	Light Chain	IGKV1	Kappa	frizzled-7	SAbDab (2024-12-13)
B, E [auth G]	Heavy Chain	IGHV3	-	frizzled-7	SAbDab (2024-12-13)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
C, F [auth H]	PF01392	Fz domain (Fz)	Fz domain	Also known as the CRD (cysteine rich domain), the C6 box in MuSK receptor. This domain of unknown function has been independently identified by several groups [1,2,3,4]. The domain contains 10 conserved cysteines.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, D [auth E]	Antibody Fab F6, Light chain	-	-	-
B, E [auth G]	Antibody Fab F6, Heavy chain	-	-	-
C, F [auth H]	Frizzled-7	molecular transducer activity transmembrane signaling receptor activity signaling receptor activity Wnt receptor activity G protein-coupled receptor activity protein binding binding PDZ domain binding protein domain specific binding signaling receptor binding G protein-coupled receptor binding frizzled binding ion binding phosphatidylinositol-4,5-bisphosphate binding molecular transducer activity transmembrane signaling receptor activity signaling receptor activity Wnt receptor activity G protein-coupled receptor activity protein binding binding PDZ domain binding protein domain specific binding signaling receptor binding G protein-coupled receptor binding frizzled binding ion binding phosphatidylinositol-4,5-bisphosphate binding anion binding phosphatidylinositol bisphosphate binding phospholipid binding lipid binding phosphatidylinositol phosphate binding phosphatidylinositol binding small molecule binding Wnt-protein binding	cellular response to stimulus regulation of biological process regulation of signaling regulation of cellular process cell surface receptor signaling pathway response to stimulus regulation of Wnt signaling pathway signaling regulation of canonical Wnt signaling pathway canonical Wnt signaling pathway biological regulation regulation of signal transduction cell communication regulation of response to stimulus cellular response to stimulus regulation of biological process regulation of signaling regulation of cellular process cell surface receptor signaling pathway response to stimulus regulation of Wnt signaling pathway signaling regulation of canonical Wnt signaling pathway canonical Wnt signaling pathway biological regulation regulation of signal transduction cell communication regulation of response to stimulus regulation of cell communication cellular process signal transduction Wnt signaling pathway wound healing cell population proliferation positive regulation of cell population proliferation positive regulation of epithelial cell proliferation positive regulation of biological process response to stress regulation of cell population proliferation regulation of epithelial cell proliferation response to wounding epithelial cell proliferation positive regulation of cellular process positive regulation of epithelial cell proliferation involved in wound healing multicellular organismal process regulation of developmental process cellular developmental process negative regulation of biological process negative regulation of muscle cell differentiation regulation of cell differentiation regulation of striated muscle cell differentiation regulation of cardiac muscle cell differentiation muscle cell differentiation striated muscle tissue development muscle structure development multicellular organism development cell differentiation negative regulation of cardiac muscle cell differentiation anatomical structure development negative regulation of cellular process negative regulation of cell differentiation regulation of multicellular organismal process muscle tissue development negative regulation of cardiocyte differentiation system development developmental process heart development cardiac muscle tissue development negative regulation of developmental process negative regulation of striated muscle cell differentiation animal organ development cardiac muscle cell differentiation tissue development cardiocyte differentiation circulatory system development regulation of muscle cell differentiation regulation of cardiocyte differentiation striated muscle cell differentiation negative regulation of multicellular organismal process non-canonical Wnt signaling pathway cell adhesion substrate adhesion-dependent cell spreading cell-substrate adhesion response to lipid response to oxygen-containing compound cellular response to oxygen-containing compound response to retinoic acid cellular response to chemical stimulus cellular response to retinoic acid response to chemical cellular response to lipid nervous system development generation of neurons neurogenesis neuron differentiation hemopoiesis cell activation cell development mononuclear cell differentiation lymphocyte activation lymphocyte differentiation leukocyte activation T cell differentiation in thymus T cell activation immune system process T cell differentiation leukocyte differentiation positive regulation of cell communication positive regulation of signal transduction intracellular signal transduction positive regulation of response to stimulus positive regulation of JNK cascade regulation of intracellular signal transduction JNK cascade intracellular signaling cassette positive regulation of MAPK cascade regulation of JNK cascade positive regulation of intracellular signal transduction regulation of MAPK cascade MAPK cascade positive regulation of signaling Wnt signaling pathway, planar cell polarity pathway RNA metabolic process regulation of primary metabolic process nucleobase-containing compound metabolic process RNA biosynthetic process nucleic acid biosynthetic process DNA-templated transcription regulation of RNA metabolic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of biosynthetic process primary metabolic process regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of gene expression biosynthetic process metabolic process regulation of macromolecule biosynthetic process regulation of DNA-templated transcription macromolecule metabolic process regulation of metabolic process maintenance of cell number stem cell population maintenance regulation of cell-substrate adhesion regulation of cell adhesion negative regulation of cell adhesion negative regulation of cell-substrate adhesion positive regulation of DNA-templated transcription positive regulation of macromolecule biosynthetic process positive regulation of metabolic process positive regulation of biosynthetic process positive regulation of macromolecule metabolic process positive regulation of nucleobase-containing compound metabolic process positive regulation of RNA metabolic process positive regulation of RNA biosynthetic process positive regulation of phosphorylation positive regulation of phosphorus metabolic process phosphorylation positive regulation of phosphate metabolic process regulation of phosphate metabolic process phosphate-containing compound metabolic process regulation of phosphorus metabolic process regulation of phosphorylation phosphorus metabolic process somatic stem cell division stem cell division cell division negative regulation of cell fate specification embryo development regulation of ectodermal cell fate specification negative regulation of cell fate commitment ectoderm development ectodermal cell differentiation negative regulation of ectodermal cell fate specification formation of primary germ layer ectodermal cell fate commitment regulation of cell fate commitment embryonic morphogenesis ectodermal cell fate specification cell fate specification gastrulation ectoderm formation cell fate commitment involved in formation of primary germ layer anatomical structure morphogenesis regulation of cell fate specification anatomical structure formation involved in morphogenesis cell fate commitment epithelium development mesenchymal to epithelial transition epithelial cell differentiation tissue regeneration skeletal muscle tissue regeneration developmental growth skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration growth regeneration	cellular anatomical structure membrane cell periphery plasma membrane membrane-bounded organelle intracellular organelle intracellular anatomical structure organelle intracellular membrane-bounded organelle endosome membrane endosome intracellular vesicle recycling endosome endomembrane system cellular anatomical structure membrane cell periphery plasma membrane membrane-bounded organelle intracellular organelle intracellular anatomical structure organelle intracellular membrane-bounded organelle endosome membrane endosome intracellular vesicle recycling endosome endomembrane system cytoplasmic vesicle membrane bounding membrane of organelle recycling endosome membrane vesicle membrane cytoplasmic vesicle cytoplasm vesicle organelle membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
C, F [auth H]	IPR036790	Frizzled cysteine-rich domain superfamily	Homologous Superfamily
C, F [auth H]	IPR042742	Frizzled-7, cysteine-rich Wnt-binding domain	Domain
C, F [auth H]	IPR017981	GPCR, family 2-like, 7TM	Domain
C, F [auth H]	IPR015526	Frizzled/secreted frizzled-related protein	Family
C, F [auth H]	IPR000539	Frizzled/Smoothened, 7TM	Domain
C, F [auth H]	IPR020067	Frizzled domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
C, F [auth H]	Pharos: O75084	pancreatic ductal adenocarcinoma pituitary cancer subependymal giant cell astrocytoma acute myeloid leukemia atypical teratoid rhabdoid tumor chronic kidney disease glioblastoma malignant colon neoplasm myopathy osteosarcoma pancreatic intraductal papillary-mucinous neoplasm with low grade dysplasia psoriasis ulcerative colitis astrocytic tumor breast cancer pancreatic ductal adenocarcinoma pituitary cancer subependymal giant cell astrocytoma acute myeloid leukemia atypical teratoid rhabdoid tumor chronic kidney disease glioblastoma malignant colon neoplasm myopathy osteosarcoma pancreatic intraductal papillary-mucinous neoplasm with low grade dysplasia psoriasis ulcerative colitis astrocytic tumor breast cancer Crohn disease interstitial cystitis malignant ependymoma nasopharyngeal carcinoma ovarian cancer Pick disease Sjogren syndrome attention deficit hyperactivity disorder, inattentive type breast carcinoma cystic fibrosis lung cancer medulloblastoma oligodendroglioma

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.