Annotations: 5C2K

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	GTPase activation domain, GAP	8079475	3001163	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Ras-like P-loop GTPases	8057414	3002022	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	RhoGAP	e5c2kA1	A: alpha bundles	X: GTPase activation domain, GAP (From Topology)	H: GTPase activation domain, GAP (From Topology)	T: GTPase activation domain, GAP	F: RhoGAP	ECOD (1.6)
A	Ras	e5c2kA2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Ras	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00071	Ras family (Ras)	Ras family	Includes sub-families Ras, Rab, Rac, Ral, Ran, Rap Ypt1 and more. Shares P-loop motif with GTP_EFTU, arf and myosin_head. See Pfam:PF00009 Pfam:PF00025, Pfam:PF00063. As regards Rab GTPases, these are important regulators of vesicle formation, motil ...	Includes sub-families Ras, Rab, Rac, Ral, Ran, Rap Ypt1 and more. Shares P-loop motif with GTP_EFTU, arf and myosin_head. See Pfam:PF00009 Pfam:PF00025, Pfam:PF00063. As regards Rab GTPases, these are important regulators of vesicle formation, motility and fusion. They share a fold in common with all Ras GTPases: this is a six-stranded beta-sheet surrounded by five alpha-helices [1].	Domain
A	PF00620	RhoGAP domain (RhoGAP)	RhoGAP domain	GTPase activator proteins towards Rho/Rac/Cdc42-like small GTPases.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Transforming protein RhoA,Rac GTPase-activating protein 1	GTPase regulator activity enzyme regulator activity enzyme activator activity GTPase activator activity molecular function activator activity nucleoside-triphosphatase regulator activity molecular function regulator activity protein-macromolecule adaptor activity molecular adaptor activity cytoskeletal protein binding protein binding binding tubulin binding alpha-tubulin binding GTPase regulator activity enzyme regulator activity enzyme activator activity GTPase activator activity molecular function activator activity nucleoside-triphosphatase regulator activity molecular function regulator activity protein-macromolecule adaptor activity molecular adaptor activity cytoskeletal protein binding protein binding binding tubulin binding alpha-tubulin binding gamma-tubulin binding microtubule binding kinase binding enzyme binding protein kinase binding ion binding phosphatidylinositol-3,4,5-trisphosphate binding anion binding phospholipid binding lipid binding phosphatidylinositol phosphate binding phosphatidylinositol binding small molecule binding beta-tubulin binding cation binding metal ion binding pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding organic cyclic compound binding purine nucleotide binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding myosin binding G protein activity	mitotic spindle elongation spindle midzone assembly spindle elongation microtubule cytoskeleton organization membraneless organelle assembly mitotic cell cycle cellular component organization cellular component biogenesis cellular component assembly mitotic sister chromatid segregation cell cycle cellular component organization or biogenesis chromosome segregation microtubule cytoskeleton organization involved in mitosis mitotic spindle elongation spindle midzone assembly spindle elongation microtubule cytoskeleton organization membraneless organelle assembly mitotic cell cycle cellular component organization cellular component biogenesis cellular component assembly mitotic sister chromatid segregation cell cycle cellular component organization or biogenesis chromosome segregation microtubule cytoskeleton organization involved in mitosis spindle organization cell cycle process mitotic nuclear division organelle assembly organelle fission chromosome organization sister chromatid segregation spindle assembly mitotic spindle organization mitotic spindle assembly organelle organization nuclear division mitotic cell cycle process nuclear chromosome segregation cellular process microtubule-based process mitotic spindle midzone assembly cytoskeleton organization developmental process neuroblast proliferation multicellular organismal process cell population proliferation cellular developmental process nervous system development generation of neurons neurogenesis multicellular organism development cell differentiation anatomical structure development neural precursor cell proliferation system development regulation of cytokinesis regulation of biological process regulation of cellular process positive regulation of cell division regulation of cell cycle process biological regulation positive regulation of biological process cytokinesis positive regulation of cell cycle regulation of cell division positive regulation of cytokinesis positive regulation of cell cycle process cell division regulation of cell cycle positive regulation of cellular process spermatogenesis sexual reproduction reproductive process multicellular organismal reproductive process developmental process involved in reproduction male gamete generation gamete generation cytoskeleton-dependent cytokinesis mitotic cytokinesis localization transport monoatomic ion transport establishment of localization cellular response to stimulus regulation of signaling small GTPase-mediated signal transduction response to stimulus signaling regulation of small GTPase mediated signal transduction regulation of signal transduction intracellular signal transduction cell communication regulation of intracellular signal transduction regulation of response to stimulus intracellular signaling cassette regulation of cell communication signal transduction homeostatic process hemopoiesis cell development erythrocyte differentiation multicellular organismal-level homeostasis myeloid cell differentiation immune system process homeostasis of number of cells myeloid cell homeostasis erythrocyte homeostasis regulation of embryonic development regulation of multicellular organismal development regulation of developmental process embryo development regulation of multicellular organismal process assembly of actomyosin apparatus involved in cytokinesis actin cytoskeleton organization actomyosin structure organization cortical cytoskeleton organization actomyosin contractile ring assembly cortical actin cytoskeleton organization actin filament-based process cytokinetic process actomyosin contractile ring organization Rho protein signal transduction establishment of organelle localization cellular localization attachment of spindle microtubules to kinetochore metaphase chromosome alignment chromosome localization organelle localization establishment of localization in cell regulation of attachment of spindle microtubules to kinetochore establishment of chromosome localization membrane organization cleavage furrow formation membrane invagination plasma membrane invagination head development forebrain generation of neurons forebrain development animal organ development brain development forebrain radial glial cell differentiation gliogenesis central nervous system development glial cell differentiation radial glial cell differentiation regulation of cell population proliferation regulation of osteoblast proliferation osteoblast proliferation response to peptide response to chemical response to chemokine response to cytokine cellular response to cytokine stimulus cellular response to chemokine intracellular receptor signaling pathway response to lipid hormone-mediated signaling pathway cellular response to endogenous stimulus response to hormone response to steroid hormone androgen receptor signaling pathway nuclear receptor-mediated steroid hormone signaling pathway cellular response to steroid hormone stimulus response to organic cyclic compound steroid hormone receptor signaling pathway cellular response to hormone stimulus response to endogenous stimulus cellular response to organic cyclic compound cellular response to chemical stimulus cellular response to lipid nuclear receptor-mediated signaling pathway endothelial cell migration cell migration cell motility cell adhesion substrate adhesion-dependent cell spreading cell-substrate adhesion cerebral cortex cell migration pallium development cerebral cortex development telencephalon cell migration forebrain cell migration telencephalon development anatomical structure morphogenesis cell morphogenesis chemotaxis negative chemotaxis taxis response to external stimulus locomotion odontogenesis animal organ morphogenesis negative regulation of signaling negative regulation of intracellular signal transduction negative regulation of biological process negative regulation of cell communication negative regulation of intracellular steroid hormone receptor signaling pathway regulation of intracellular steroid hormone receptor signaling pathway negative regulation of cellular process negative regulation of response to stimulus negative regulation of signal transduction negative regulation of locomotion regulation of cell motility cell migration involved in sprouting angiogenesis regulation of blood vessel endothelial cell migration angiogenesis negative regulation of endothelial cell migration negative regulation of blood vessel endothelial cell migration tube morphogenesis regulation of cell migration blood vessel endothelial cell migration regulation of endothelial cell migration negative regulation of cell migration blood vessel development blood vessel morphogenesis tube development regulation of cell migration involved in sprouting angiogenesis sprouting angiogenesis vasculature development circulatory system development regulation of locomotion anatomical structure formation involved in morphogenesis negative regulation of cell motility negative regulation of cell migration involved in sprouting angiogenesis regulation of protein modification process positive regulation of phosphorylation regulation of catalytic activity regulation of protein phosphorylation positive regulation of protein metabolic process regulation of transferase activity positive regulation of phosphate metabolic process regulation of primary metabolic process positive regulation of protein serine/threonine kinase activity positive regulation of metabolic process positive regulation of molecular function positive regulation of protein modification process regulation of phosphate metabolic process phosphate-containing compound metabolic process positive regulation of macromolecule metabolic process protein metabolic process positive regulation of protein kinase activity phosphorus metabolic process protein modification process positive regulation of protein phosphorylation protein phosphorylation positive regulation of transferase activity positive regulation of phosphorus metabolic process phosphorylation regulation of protein metabolic process positive regulation of catalytic activity primary metabolic process regulation of protein kinase activity regulation of macromolecule metabolic process regulation of molecular function positive regulation of kinase activity regulation of kinase activity macromolecule modification regulation of protein serine/threonine kinase activity regulation of phosphorylation regulation of phosphorus metabolic process metabolic process macromolecule metabolic process regulation of metabolic process heart development heart valve development aortic valve development heart valve morphogenesis aortic valve formation semi-lunar valve development aortic valve morphogenesis heart valve formation regulation of leukocyte differentiation regulation of leukocyte activation regulation of alpha-beta T cell activation regulation of cell activation mononuclear cell differentiation regulation of cell differentiation lymphocyte differentiation positive regulation of T cell differentiation regulation of cell development positive regulation of developmental process positive regulation of cell activation T cell activation positive regulation of leukocyte activation alpha-beta T cell activation T cell differentiation regulation of immune system process positive regulation of immune system process positive regulation of T cell activation positive regulation of cell development positive regulation of alpha-beta T cell activation regulation of leukocyte cell-cell adhesion positive regulation of multicellular organismal process leukocyte differentiation positive regulation of alpha-beta T cell differentiation positive regulation of leukocyte cell-cell adhesion regulation of alpha-beta T cell differentiation lymphocyte activation leukocyte cell-cell adhesion leukocyte activation positive regulation of cell-cell adhesion regulation of T cell differentiation positive regulation of lymphocyte activation alpha-beta T cell differentiation cell-cell adhesion positive regulation of hemopoiesis positive regulation of cell differentiation regulation of cell-cell adhesion regulation of lymphocyte differentiation positive regulation of cell adhesion regulation of cell adhesion cell activation regulation of T cell activation regulation of hemopoiesis positive regulation of leukocyte differentiation positive regulation of lymphocyte differentiation regulation of lymphocyte activation alpha-beta T cell lineage commitment T cell lineage commitment cell fate commitment positive regulation of cell communication positive regulation of signal transduction positive regulation of canonical NF-kappaB signal transduction positive regulation of response to stimulus positive regulation of intracellular signal transduction canonical NF-kappaB signal transduction regulation of canonical NF-kappaB signal transduction positive regulation of signaling vascular associated smooth muscle contraction system process positive regulation of smooth muscle contraction regulation of vascular associated smooth muscle contraction regulation of system process circulatory system process smooth muscle contraction regulation of smooth muscle contraction blood vessel diameter maintenance regulation of biological quality blood circulation regulation of tube diameter regulation of tube size positive regulation of vasoconstriction muscle contraction positive regulation of vascular associated smooth muscle contraction muscle system process regulation of muscle system process regulation of blood circulation regulation of muscle contraction positive regulation of muscle contraction vascular process in circulatory system regulation of vasoconstriction vasoconstriction regulation of anatomical structure size skeletal muscle satellite cell migration regulation of systemic arterial blood pressure by endothelin regulation of systemic arterial blood pressure by hormone regulation of systemic arterial blood pressure mediated by a chemical signal regulation of blood pressure regulation of systemic arterial blood pressure endocrine process neuron apoptotic process apoptotic process motor neuron apoptotic process cell death programmed cell death regulation of cytoskeleton organization regulation of cellular component organization regulation of actin filament-based process regulation of organelle organization regulation of actin cytoskeleton organization bone trabecula morphogenesis trabecula morphogenesis positive regulation of hydrolase activity regulation of hydrolase activity regulation of lipase activity positive regulation of lipase activity polarized epithelial cell differentiation establishment of monopolar cell polarity establishment or maintenance of monopolar cell polarity establishment or maintenance of cell polarity epithelial cell differentiation morphogenesis of a polarized epithelium establishment or maintenance of apical/basal cell polarity establishment or maintenance of bipolar cell polarity epithelium development establishment of epithelial cell apical/basal polarity morphogenesis of an epithelium tissue development establishment of cell polarity tissue morphogenesis establishment or maintenance of epithelial cell apical/basal polarity establishment of apical/basal cell polarity establishment of epithelial cell polarity cytoplasmic microtubule organization supramolecular fiber organization substantia nigra development midbrain development neural nucleus development regulation of cell-substrate adhesion negative regulation of cell adhesion negative regulation of cell-substrate adhesion regulation of non-canonical NF-kappaB signal transduction non-canonical NF-kappaB signal transduction positive regulation of non-canonical NF-kappaB signal transduction cell surface receptor signaling pathway Roundabout signaling pathway regulation of modification of postsynaptic actin cytoskeleton regulation of modification of synaptic structure regulation of synapse organization cell junction organization modification of postsynaptic actin cytoskeleton regulation of synapse structure or activity regulation of modification of postsynaptic structure synapse organization modification of synaptic structure modification of postsynaptic structure regulation of neuron projection development cell projection organization neuron projection development neuron differentiation plasma membrane bounded cell projection organization regulation of cell projection organization neuron development regulation of plasma membrane bounded cell projection organization positive regulation of organelle organization positive regulation of cellular component organization regulation of actin filament bundle assembly positive regulation of actin filament bundle assembly actin filament bundle organization positive regulation of stress fiber assembly stress fiber assembly regulation of actin filament organization positive regulation of cellular component biogenesis positive regulation of cytoskeleton organization actin filament organization regulation of cellular component biogenesis positive regulation of supramolecular fiber organization regulation of stress fiber assembly contractile actin filament bundle assembly regulation of actomyosin structure organization actin filament bundle assembly regulation of supramolecular fiber organization negative regulation of cell size regulation of cellular component size regulation of cell size positive regulation of leukocyte adhesion to vascular endothelial cell regulation of leukocyte adhesion to vascular endothelial cell leukocyte adhesion to vascular endothelial cell cell-substrate junction assembly cell junction assembly regulation of cell-substrate junction organization focal adhesion assembly regulation of cell junction assembly cell-matrix adhesion regulation of cell-matrix adhesion cell-substrate junction organization regulation of focal adhesion assembly regulation of cell-substrate junction assembly beta selection T cell selection regulation of neural precursor cell proliferation apical junction assembly cell-cell junction assembly cell-cell junction organization Wnt signaling pathway, planar cell polarity pathway non-canonical Wnt signaling pathway Wnt signaling pathway regulation of systemic arterial blood pressure by renin-angiotensin positive regulation of systemic arterial blood pressure positive regulation of blood pressure renal control of peripheral vascular resistance involved in regulation of systemic arterial blood pressure renal system process involved in regulation of systemic arterial blood pressure maintenance of blood vessel diameter homeostasis by renin-angiotensin renal system process angiotensin-mediated vasoconstriction involved in regulation of systemic arterial blood pressure positive regulation of locomotion regulation of lymphocyte migration positive regulation of cell migration positive regulation of cell motility positive regulation of mononuclear cell migration regulation of mononuclear cell migration positive regulation of lymphocyte migration regulation of leukocyte migration positive regulation of T cell migration lymphocyte migration positive regulation of leukocyte migration leukocyte migration regulation of T cell migration mononuclear cell migration T cell migration positive regulation of podosome assembly positive regulation of protein-containing complex assembly regulation of protein-containing complex assembly regulation of podosome assembly protein-containing complex assembly podosome assembly protein-containing complex organization regulation of organelle assembly positive regulation of organelle assembly endothelium development morphogenesis of an endothelium endothelial tube lumen extension endothelial tube morphogenesis epithelial tube morphogenesis kidney development renal system development apolipoprotein A-I-mediated signaling pathway reactive oxygen species biosynthetic process regulation of biosynthetic process negative regulation of metabolic process reactive oxygen species metabolic process regulation of reactive oxygen species biosynthetic process negative regulation of reactive oxygen species metabolic process biosynthetic process negative regulation of reactive oxygen species biosynthetic process negative regulation of biosynthetic process regulation of reactive oxygen species metabolic process biological process involved in interspecies interaction between organisms response to other organism cellular response to lipopolysaccharide response to oxygen-containing compound cellular response to molecule of bacterial origin response to lipopolysaccharide cellular response to oxygen-containing compound response to biotic stimulus cellular response to biotic stimulus response to bacterium response to external biotic stimulus response to molecule of bacterial origin muscle structure development skeletal muscle organ development skeletal muscle tissue development muscle tissue development muscle organ development striated muscle tissue development aerobic respiration cellular respiration oxidative phosphorylation energy derivation by oxidation of organic compounds negative regulation of cellular respiration regulation of generation of precursor metabolites and energy generation of precursor metabolites and energy regulation of cellular respiration regulation of aerobic respiration regulation of oxidative phosphorylation negative regulation of oxidative phosphorylation regulation of microtubule-based process regulation of microtubule cytoskeleton organization mitotic cytokinetic process mitotic cleavage furrow formation positive regulation of neuron differentiation regulation of neuron differentiation semaphorin-plexin signaling pathway bone maturation developmental maturation skeletal system development ossification anatomical structure maturation ossification involved in bone maturation animal organ maturation bone development neuron migration RNA metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process regulation of RNA metabolic process transcription by RNA polymerase II regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of transcription by RNA polymerase II nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of gene expression regulation of macromolecule biosynthetic process regulation of DNA-templated transcription wound healing epiboly involved in wound healing morphogenesis of an epithelial sheet response to stress epiboly wound healing, spreading of cells response to wounding negative regulation of neuron apoptotic process negative regulation of programmed cell death regulation of apoptotic process regulation of programmed cell death regulation of motor neuron apoptotic process negative regulation of apoptotic process negative regulation of motor neuron apoptotic process regulation of neuron apoptotic process	membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle midbody cytoskeleton spindle membraneless organelle intracellular membraneless organelle spindle midzone membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle midbody cytoskeleton spindle membraneless organelle intracellular membraneless organelle spindle midzone microtubule cytoskeleton extracellular exosome extracellular vesicle extracellular organelle extracellular region extracellular space vesicle extracellular membrane-bounded organelle cytosol mitotic spindle cell division site cell periphery cleavage furrow membrane plasma membrane plasma membrane region nucleus membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen protein-containing complex centralspindlin complex intracellular protein-containing complex Flemming body cytoplasmic side of plasma membrane cytoplasmic side of membrane side of membrane polymeric cytoskeletal fiber supramolecular fiber microtubule supramolecular polymer supramolecular complex acrosomal vesicle intracellular vesicle endomembrane system cytoplasmic vesicle secretory granule secretory vesicle apical junction complex cell-cell junction anchoring junction cell junction neuron spine synapse neuron projection dendritic spine plasma membrane bounded cell projection somatodendritic compartment cell projection postsynapse dendrite dendritic tree focal adhesion cell-substrate junction ruffle cell leading edge leading edge membrane ruffle membrane cell projection membrane organelle subcompartment endoplasmic reticulum subcompartment nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum organelle membrane endoplasmic reticulum membrane cytoplasmic vesicle membrane bounding membrane of organelle vesicle membrane secretory granule membrane endosome ficolin-1-rich granule membrane ficolin-1-rich granule tertiary granule lamellipodium glutamatergic synapse cell cortex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR008936	Rho GTPase activation protein	Homologous Superfamily
A	IPR000198	Rho GTPase-activating protein domain	Domain
A	IPR002219	Protein kinase C-like, phorbol ester/diacylglycerol-binding domain	Domain
A	IPR046349	C1-like domain superfamily	Homologous Superfamily
A	IPR001806	Small GTPase	Family
A	IPR003578	Small GTPase Rho	Family
A	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
A	IPR005225	Small GTP-binding domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: P61586	polycystic ovary syndrome hepatocellular carcinoma non-small cell lung carcinoma pancreatic intraductal papillary-mucinous carcinoma pituitary cancer Sclerocystic Ovaries adrenal cortex carcinoma tuberculosis breast cancer ductal breast carcinoma in situ large cell medulloblastoma malignant colon neoplasm medulloblastoma oligodendroglioma pancreatic intraductal papillary-mucinous neoplasm polycystic ovary syndrome hepatocellular carcinoma non-small cell lung carcinoma pancreatic intraductal papillary-mucinous carcinoma pituitary cancer Sclerocystic Ovaries adrenal cortex carcinoma tuberculosis breast cancer ductal breast carcinoma in situ large cell medulloblastoma malignant colon neoplasm medulloblastoma oligodendroglioma pancreatic intraductal papillary-mucinous neoplasm sinusitis allergic rhinitis breast carcinoma endometriosis IgA glomerulonephritis lung adenocarcinoma malignant glioma nasopharyngeal carcinoma ovarian cancer pancreatic intraductal papillary-mucinous neoplasm with low grade dysplasia primitive neuroectodermal tumor Sjogren syndrome atopic eczema cystic fibrosis glioblastoma invasive ductal breast carcinoma lung cancer malignant pancreatic neoplasm mature T-cell and NK-cell non-Hodgkin lymphoma carcinoma primary cutaneous T-cell non-Hodgkin lymphoma sarcomatoid carcinoma proteinuria T-cell non-Hodgkin lymphoma urinary bladder cancer Neoplasm Invasiveness angioimmunoblastic T-cell lymphoma cancer heart disease IgA glomerulonephritis osteoporosis breast disease pancreatic ductal adenocarcinoma exocrine pancreatic carcinoma undifferentiated carcinoma gastrointestinal stromal tumor hereditary diffuse gastric adenocarcinoma lung carcinoma lymphoplasmacytic lymphoma malignant pancreatic neoplasm myopathy osteosarcoma Burkitt lymphoma endogenous depression gastric cancer granulomatous slack skin disease lymphoma malignant ependymoma MALT lymphoma ovarian cancer congenital diaphragmatic hernia plasma cell myeloma diabetes mellitus rheumatoid arthritis endometriosis toxic or drug-related embryofetopathy gastric neoplasm urinary bladder neoplasm

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.