4DJF

Crystal structure of folate-bound corrinoid iron-sulfur protein (CFeSP) in complex with its methyltransferase (MeTr), co-crystallized with folate and Ti(III) citrate reductant

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d4djfa_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Dihydropteroate synthetase-like	Methyltetrahydrofolate-utiluzing methyltransferases	automated matches	(Moorella thermoacetica ) [TaxId: 1525 ],	SCOPe (2.08)
B	d4djfb_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Dihydropteroate synthetase-like	Methyltetrahydrofolate-utiluzing methyltransferases	automated matches	(Moorella thermoacetica ) [TaxId: 1525 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
C	SCOP2B Superfamily	AcsC Fe-S domain-like	8089862	3002395	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	AcsC C-terminal domain-like	8089872	3002396	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Dihydropteroate synthetase-like	8089870	3000643	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	AcsC C-terminal domain-like	8089872	3002396	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	Dihydropteroate synthetase-like	8089870	3000643	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	AcsC Fe-S domain-like	8089862	3002395	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Dihydropteroate synthetase-like	8089873	3000643	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Dihydropteroate synthetase-like	8089873	3000643	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Dihydropteroate synthetase-like	8043870	3000643	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Dihydropteroate synthetase-like	8043870	3000643	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
C	CdhD_N	e4djfC4	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: CdhD_N	ECOD (1.6)
C	CdhD_C	e4djfC1	A: a/b three-layered sandwiches	X: B12 binding domain in carbon monoxide dehydrogenase corrinoid/iron-sulfur protein gamma subunit (From Topology)	H: B12 binding domain in carbon monoxide dehydrogenase corrinoid/iron-sulfur protein gamma subunit (From Topology)	T: B12 binding domain in carbon monoxide dehydrogenase corrinoid/iron-sulfur protein gamma subunit	F: CdhD_C	ECOD (1.6)
C	FeS	e4djfC3	A: few secondary structure elements	X: Fe-S cluster binding domain in carbon monoxide dehydrogenase corrinoid/iron-sulfur protein gamma subunit (From Topology)	H: Fe-S cluster binding domain in carbon monoxide dehydrogenase corrinoid/iron-sulfur protein gamma subunit (From Topology)	T: Fe-S cluster binding domain in carbon monoxide dehydrogenase corrinoid/iron-sulfur protein gamma subunit	F: FeS	ECOD (1.6)
E	CdhD_N	e4djfE4	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: CdhD_N	ECOD (1.6)
E	CdhD_C	e4djfE1	A: a/b three-layered sandwiches	X: B12 binding domain in carbon monoxide dehydrogenase corrinoid/iron-sulfur protein gamma subunit (From Topology)	H: B12 binding domain in carbon monoxide dehydrogenase corrinoid/iron-sulfur protein gamma subunit (From Topology)	T: B12 binding domain in carbon monoxide dehydrogenase corrinoid/iron-sulfur protein gamma subunit	F: CdhD_C	ECOD (1.6)
E	FeS	e4djfE3	A: few secondary structure elements	X: Fe-S cluster binding domain in carbon monoxide dehydrogenase corrinoid/iron-sulfur protein gamma subunit (From Topology)	H: Fe-S cluster binding domain in carbon monoxide dehydrogenase corrinoid/iron-sulfur protein gamma subunit (From Topology)	T: Fe-S cluster binding domain in carbon monoxide dehydrogenase corrinoid/iron-sulfur protein gamma subunit	F: FeS	ECOD (1.6)
D	CdhD_N	e4djfD1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: CdhD_N	ECOD (1.6)
F	CdhD_N	e4djfF1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: CdhD_N	ECOD (1.6)
A	Pterin_bind	e4djfA1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Pterin_bind	ECOD (1.6)
B	Pterin_bind	e4djfB1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Pterin_bind	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
C	3.20.20.20	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Dihydropteroate synthase-like	CATH (4.3.0)
C	3.40.50.11600	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold		CATH (4.3.0)
E	3.20.20.20	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Dihydropteroate synthase-like	CATH (4.3.0)
E	3.40.50.11600	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold		CATH (4.3.0)
D	3.20.20.20	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Dihydropteroate synthase-like	CATH (4.3.0)
F	3.20.20.20	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Dihydropteroate synthase-like	CATH (4.3.0)
A	3.20.20.20	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Dihydropteroate synthase-like	CATH (4.3.0)
B	3.20.20.20	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Dihydropteroate synthase-like	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
C, E	PF04060	Putative Fe-S cluster (FeS)	Putative Fe-S cluster	This family includes a domain with four conserved cysteines that probably form an Fe-S redox cluster.	Domain
C, E	PF03599	CO dehydrogenase/acetyl-CoA synthase delta subunit (CdhD)	CO dehydrogenase/acetyl-CoA synthase delta subunit	-	Family
D, F	PF03599	CO dehydrogenase/acetyl-CoA synthase delta subunit (CdhD)	CO dehydrogenase/acetyl-CoA synthase delta subunit	-	Family
A, B	PF00809	Pterin binding enzyme (Pterin_bind)	Pterin binding enzyme	This family includes a variety of pterin binding enzymes that all adopt a TIM barrel fold. The family includes dihydropteroate synthase EC:2.5.1.15 as well as a group methyltransferase enzymes including methyltetrahydrofolate, corrinoid iron-sulfur p ...	This family includes a variety of pterin binding enzymes that all adopt a TIM barrel fold. The family includes dihydropteroate synthase EC:2.5.1.15 as well as a group methyltransferase enzymes including methyltetrahydrofolate, corrinoid iron-sulfur protein methyltransferase (MeTr) Swiss:Q46389 that catalyses a key step in the Wood-Ljungdahl pathway of carbon dioxide fixation. It transfers the N5-methyl group from methyltetrahydrofolate (CH3-H4folate) to a cob(I)amide centre in another protein, the corrinoid iron-sulfur protein. MeTr is a member of a family of proteins that includes methionine synthase and methanogenic enzymes that activate the methyl group of methyltetra-hydromethano(or -sarcino)pterin [2].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
C, E	Corrinoid/iron-sulfur protein large subunit	vitamin binding binding cobalamin binding organic cyclic compound binding tetrapyrrole binding heterocyclic compound binding small molecule binding transferase activity, transferring one-carbon groups transferase activity methyltransferase activity catalytic activity iron-sulfur cluster binding 4 iron, 4 sulfur cluster binding metal cluster binding vitamin binding binding cobalamin binding organic cyclic compound binding tetrapyrrole binding heterocyclic compound binding small molecule binding transferase activity, transferring one-carbon groups transferase activity methyltransferase activity catalytic activity iron-sulfur cluster binding 4 iron, 4 sulfur cluster binding metal cluster binding cation binding transition metal ion binding iron ion binding ion binding metal ion binding	catabolic process organophosphate catabolic process nucleoside phosphate catabolic process acetyl-CoA metabolic process nucleobase-containing compound metabolic process cellular catabolic process acyl-CoA metabolic process nucleobase-containing small molecule metabolic process phosphate-containing compound metabolic process small molecule metabolic process sulfur compound metabolic process amide metabolic process thioester metabolic process phosphorus metabolic process catabolic process organophosphate catabolic process nucleoside phosphate catabolic process acetyl-CoA metabolic process nucleobase-containing compound metabolic process cellular catabolic process acyl-CoA metabolic process nucleobase-containing small molecule metabolic process phosphate-containing compound metabolic process small molecule metabolic process sulfur compound metabolic process amide metabolic process thioester metabolic process phosphorus metabolic process acetyl-CoA catabolic process nucleoside phosphate metabolic process primary metabolic process purine-containing compound catabolic process nucleobase-containing compound catabolic process purine-containing compound metabolic process sulfur compound catabolic process organophosphate metabolic process metabolic process cellular process carbon fixation	-
D, F	Corrinoid/iron-sulfur protein small subunit	vitamin binding binding cobalamin binding organic cyclic compound binding tetrapyrrole binding heterocyclic compound binding small molecule binding	small molecule metabolic process metabolic process cellular process one-carbon metabolic process carbon fixation	-
A, B	5-methyltetrahydrofolate corrinoid/iron sulfur protein methyltransferase	cation binding ion binding binding calcium ion binding metal ion binding small molecule binding vitamin binding cobalamin binding organic cyclic compound binding tetrapyrrole binding heterocyclic compound binding methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase activity transferase activity, transferring one-carbon groups transferase activity cation binding ion binding binding calcium ion binding metal ion binding small molecule binding vitamin binding cobalamin binding organic cyclic compound binding tetrapyrrole binding heterocyclic compound binding methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase activity transferase activity, transferring one-carbon groups transferase activity methyltransferase activity catalytic activity methionine synthase activity 5-methyltetrahydrofolate-dependent methyltransferase activity S-methyltransferase activity	methylation metabolic process cellular process pteridine-containing compound metabolic process carbon fixation	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
C, E	IPR016218	Acetyl-CoA decarbonylase/synthase complex, gamma subunit	Family
C, E	IPR016041	CO dehydrogenase/acetyl-CoA synthase delta subunit, TIM barrel	Domain
C, E	IPR007202	4Fe-4S domain	Domain
C, E	IPR011005	Dihydropteroate synthase-like superfamily	Homologous Superfamily
C, E	IPR051069	Acetyl-CoA decarbonylase/synthase complex subunit	Family
D, F	IPR016041	CO dehydrogenase/acetyl-CoA synthase delta subunit, TIM barrel	Domain
D, F	IPR004486	CO dehydrogenase/acetyl-CoA synthase delta subunit	Family
D, F	IPR011005	Dihydropteroate synthase-like superfamily	Homologous Superfamily
D, F	IPR051069	Acetyl-CoA decarbonylase/synthase complex subunit	Family
A, B	IPR050554	Methionine Synthase/Corrinoid	Family
A, B	IPR011005	Dihydropteroate synthase-like superfamily	Homologous Superfamily
A, B	IPR000489	Pterin-binding domain	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.