2WAB | pdb_00002wab

Structure of an active site mutant of a family two carbohydrate esterase from Clostridium thermocellum in complex with celluohexase

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	EUF07626	e2wabA1	A: beta sandwiches	X: jelly-roll	H: Concanavalin A-like	T: Galactose-binding domain-like	F: EUF07626	ECOD (1.6)
A	Lipase_GDSL_2_1	e2wabA2	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: SGNH hydrolase (From Topology)	T: SGNH hydrolase	F: Lipase_GDSL_2_1	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.60.120.260	Mainly Beta	Sandwich	Jelly Rolls	Galactose-binding domain-like	CATH (4.3.0)
A	3.40.50.1110	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	SGNH hydrolase	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00657	GDSL-like Lipase/Acylhydrolase (Lipase_GDSL)	GDSL-like Lipase/Acylhydrolase	-	Family
A	PF17996	Carbohydrate esterase 2 N-terminal (CE2_N)	Carbohydrate esterase 2 N-terminal	This is the N-terminal beta-sheet domain with jelly roll topology found in CE2 acetyl-esterase from the bacterium Clostridium thermocellum. This enzyme displays dual activities, it catalyses the deacetylation of plant polysaccharides and also potenti ...	This is the N-terminal beta-sheet domain with jelly roll topology found in CE2 acetyl-esterase from the bacterium Clostridium thermocellum. This enzyme displays dual activities, it catalyses the deacetylation of plant polysaccharides and also potentiates the activity of its appended cellulase catalytic module through its noncatalytic cellulose binding function. This N-terminal jelly-roll domain appears to extend the substrate/cellulose binding cleft of the catalytic domain in C.thermocellum [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	ENDOGLUCANASE E	hydrolase activity, acting on glycosyl bonds cellulase activity hydrolase activity, hydrolyzing O-glycosyl compounds hydrolase activity catalytic activity binding polysaccharide binding cellulose binding carbohydrate binding hydrolase activity, acting on ester bonds carboxylic ester hydrolase activity acetylxylan esterase activity cation binding ion binding hydrolase activity, acting on glycosyl bonds cellulase activity hydrolase activity, hydrolyzing O-glycosyl compounds hydrolase activity catalytic activity binding polysaccharide binding cellulose binding carbohydrate binding hydrolase activity, acting on ester bonds carboxylic ester hydrolase activity acetylxylan esterase activity cation binding ion binding metal ion binding small molecule binding	carbohydrate metabolic process xylan catabolic process catabolic process hemicellulose metabolic process cell wall organization or biogenesis carbohydrate catabolic process hemicellulose catabolic process primary metabolic process macromolecule catabolic process cell wall macromolecule catabolic process xylan metabolic process polysaccharide metabolic process polysaccharide catabolic process metabolic process carbohydrate metabolic process xylan catabolic process catabolic process hemicellulose metabolic process cell wall organization or biogenesis carbohydrate catabolic process hemicellulose catabolic process primary metabolic process macromolecule catabolic process cell wall macromolecule catabolic process xylan metabolic process polysaccharide metabolic process polysaccharide catabolic process metabolic process cellular process macromolecule metabolic process cell wall polysaccharide metabolic process cell wall macromolecule metabolic process cell wall polysaccharide catabolic process glucomannan catabolic process substituted mannan metabolic process glucomannan metabolic process glucan catabolic process beta-glucan metabolic process glucan metabolic process beta-glucan catabolic process cellulose catabolic process cellulose metabolic process	cellular anatomical structure extracellular region

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR002105	Dockerin type I repeat	Repeat
A	IPR001087	GDSL lipase/esterase	Family
A	IPR052762	Plant cell wall deacetylase/carbohydrate esterase	Family
A	IPR016134	Dockerin domain	Domain
A	IPR017853	Glycoside hydrolase superfamily	Homologous Superfamily
A	IPR036514	SGNH hydrolase superfamily	Homologous Superfamily
A	IPR001547	Glycoside hydrolase, family 5	Domain
A	IPR037461	CtCE2-like domain	Domain
A	IPR040794	Carbohydrate esterase 2, N-terminal	Domain
A	IPR036439	Dockerin domain superfamily	Homologous Superfamily
A	IPR018087	Glycoside hydrolase, family 5, conserved site	Conserved Site

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.