2RET | pdb_00002ret

The crystal structure of a binary complex of two pseudopilins: EpsI and EpsJ from the Type 2 Secretion System of Vibrio vulnificus

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2reta1	Alpha and beta proteins (a+b)	Pili subunits	Pili subunits	GSPII I/J protein-like	Pseudopilin EpsI	(Vibrio vulnificus ) [TaxId: 672 ],	SCOPe (2.08)
A	d2reta2	Artifacts	Tags	Tags	Tags	C-terminal Tags	(Vibrio vulnificus ) [TaxId: 672 ],	SCOPe (2.08)
C	d2retc2	Alpha and beta proteins (a+b)	Pili subunits	Pili subunits	GSPII I/J protein-like	Pseudopilin EpsI	(Vibrio vulnificus ) [TaxId: 672 ],	SCOPe (2.08)
C	d2retc3	Artifacts	Tags	Tags	Tags	C-terminal Tags	(Vibrio vulnificus ) [TaxId: 672 ],	SCOPe (2.08)
E	d2rete2	Alpha and beta proteins (a+b)	Pili subunits	Pili subunits	GSPII I/J protein-like	Pseudopilin EpsI	(Vibrio vulnificus ) [TaxId: 672 ],	SCOPe (2.08)
E	d2rete3	Artifacts	Tags	Tags	Tags	C-terminal Tags	(Vibrio vulnificus ) [TaxId: 672 ],	SCOPe (2.08)
G	d2retg_	Alpha and beta proteins (a+b)	Pili subunits	Pili subunits	GSPII I/J protein-like	Pseudopilin EpsI	(Vibrio vulnificus ) [TaxId: 672 ],	SCOPe (2.08)
B	d2retb1	Alpha and beta proteins (a+b)	Pili subunits	Pili subunits	EpsJ-like	Type II secretory pathway component EpsJ	(Vibrio vulnificus ) [TaxId: 672 ],	SCOPe (2.08)
D	d2retd_	Alpha and beta proteins (a+b)	Pili subunits	Pili subunits	EpsJ-like	Type II secretory pathway component EpsJ	(Vibrio vulnificus ) [TaxId: 672 ],	SCOPe (2.08)
F	d2retf_	Alpha and beta proteins (a+b)	Pili subunits	Pili subunits	EpsJ-like	Type II secretory pathway component EpsJ	(Vibrio vulnificus ) [TaxId: 672 ],	SCOPe (2.08)
H	d2reth_	Alpha and beta proteins (a+b)	Pili subunits	Pili subunits	EpsJ-like	Type II secretory pathway component EpsJ	(Vibrio vulnificus ) [TaxId: 672 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	GSPII I/J protein-like	8003826	4000249	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Pili subunits	8003827	3000097	SCOP2 (2022-06-29)
C	SCOP2B Superfamily	Pili subunits	8003827	3000097	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	Pili subunits	8003827	3000097	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	Pili subunits	8003827	3000097	SCOP2B (2022-06-29)
B	SCOP2 Family	EpsJ-like	8024506	4001771	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	Pili subunits	8036885	3000097	SCOP2 (2022-06-29)
D	SCOP2B Superfamily	Pili subunits	8036885	3000097	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Pili subunits	8036885	3000097	SCOP2B (2022-06-29)
H	SCOP2B Superfamily	Pili subunits	8036885	3000097	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	T2SSI	e2retA1	A: a+b two layers	X: Pili subunits (From Topology)	H: Pili subunits (From Topology)	T: Pili subunits	F: T2SSI	ECOD (1.6)
C	T2SSI	e2retC1	A: a+b two layers	X: Pili subunits (From Topology)	H: Pili subunits (From Topology)	T: Pili subunits	F: T2SSI	ECOD (1.6)
E	T2SSI	e2retE1	A: a+b two layers	X: Pili subunits (From Topology)	H: Pili subunits (From Topology)	T: Pili subunits	F: T2SSI	ECOD (1.6)
G	T2SSI	e2retG1	A: a+b two layers	X: Pili subunits (From Topology)	H: Pili subunits (From Topology)	T: Pili subunits	F: T2SSI	ECOD (1.6)
B	T2SSJ	e2retB1	A: a+b two layers	X: Pili subunits (From Topology)	H: Pili subunits (From Topology)	T: Pili subunits	F: T2SSJ	ECOD (1.6)
D	T2SSJ	e2retD1	A: a+b two layers	X: Pili subunits (From Topology)	H: Pili subunits (From Topology)	T: Pili subunits	F: T2SSJ	ECOD (1.6)
F	T2SSJ	e2retF1	A: a+b two layers	X: Pili subunits (From Topology)	H: Pili subunits (From Topology)	T: Pili subunits	F: T2SSJ	ECOD (1.6)
H	T2SSJ	e2retH1	A: a+b two layers	X: Pili subunits (From Topology)	H: Pili subunits (From Topology)	T: Pili subunits	F: T2SSJ	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.1300.30	Alpha Beta	2-Layer Sandwich	Pantoate--beta-alanine Ligase	Chain: A,domain 2	CATH (4.3.0)
C	3.30.1300.30	Alpha Beta	2-Layer Sandwich	Pantoate--beta-alanine Ligase	Chain: A,domain 2	CATH (4.3.0)
E	3.30.1300.30	Alpha Beta	2-Layer Sandwich	Pantoate--beta-alanine Ligase	Chain: A,domain 2	CATH (4.3.0)
G	3.30.1300.30	Alpha Beta	2-Layer Sandwich	Pantoate--beta-alanine Ligase	Chain: A,domain 2	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, C, E, G	PF02501	Type II secretion system (T2SS), protein I (T2SSI)	Type II secretion system (T2SS), protein I	The Type II secretion system, also called Secretion-dependent pathway (SDP), is responsible for the transport of proteins across the outer membrane first exported to the periplasm by the Sec or Tat translocon in Gram-negative (diderm) bacteria. As me ...	The Type II secretion system, also called Secretion-dependent pathway (SDP), is responsible for the transport of proteins across the outer membrane first exported to the periplasm by the Sec or Tat translocon in Gram-negative (diderm) bacteria. As members of the T2SJ family, members of the T2SI family are pseudopilins containing prepilin signal sequences [1].	Domain
B, D, F, H	PF11612	Type II secretion system (T2SS), protein J (T2SSJ)	Type II secretion system (T2SS), protein J	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, C, E, G	Pseudopilin EpsI	-	protein localization protein transport across the cell outer membrane establishment of protein localization cellular localization nitrogen compound transport transport secretion establishment of protein localization to extracellular region export from cell protein secretion by the type II secretion system protein secretion establishment of localization protein localization to extracellular region transmembrane transport localization protein localization protein transport across the cell outer membrane establishment of protein localization cellular localization nitrogen compound transport transport secretion establishment of protein localization to extracellular region export from cell protein secretion by the type II secretion system protein secretion establishment of localization protein localization to extracellular region transmembrane transport localization secretion by cell protein transmembrane transport protein transport cellular macromolecule localization macromolecule localization cellular process	cell periphery cellular anatomical structure plasma membrane membrane protein-containing complex type II protein secretion system complex
B, D, F, H	EpsJ	-	protein localization protein transport across the cell outer membrane establishment of protein localization cellular localization nitrogen compound transport transport secretion establishment of protein localization to extracellular region export from cell protein secretion by the type II secretion system protein secretion establishment of localization protein localization to extracellular region transmembrane transport localization protein localization protein transport across the cell outer membrane establishment of protein localization cellular localization nitrogen compound transport transport secretion establishment of protein localization to extracellular region export from cell protein secretion by the type II secretion system protein secretion establishment of localization protein localization to extracellular region transmembrane transport localization secretion by cell protein transmembrane transport protein transport cellular macromolecule localization macromolecule localization cellular process	cell periphery cellular anatomical structure plasma membrane membrane protein-containing complex type II protein secretion system complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, C, E, G	IPR003413	Type II secretion system protein GspI, C-terminal	Domain
A, C, E, G	IPR012902	Prokaryotic N-terminal methylation site	PTM
A, C, E, G	IPR010052	Type II secretion system protein GspI	Family
A, C, E, G	IPR045584	Pilin-like	Homologous Superfamily
B, D, F, H	IPR010055	Type II secretion system protein GspJ	Family
B, D, F, H	IPR012902	Prokaryotic N-terminal methylation site	PTM
B, D, F, H	IPR051621	Type II secretion system protein J	Family
B, D, F, H	IPR045584	Pilin-like	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.