2QWO | pdb_00002qwo

Crystal structure of disulfide-bond-crosslinked complex of bovine hsc70 (1-394aa)R171C and bovine Auxilin (810-910aa)D876C in the ADP*Pi form #1

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2qwoa1	Alpha and beta proteins (a/b)	Ribonuclease H-like motif	Actin-like ATPase domain	Actin/HSP70	Heat shock protein 70kDa, ATPase fragment	cattle (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)
B	d2qwob_	All alpha proteins	Long alpha-hairpin	Chaperone J-domain	Chaperone J-domain	Auxilin J-domain	cattle (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Actin-like ATPases	8044469	3000092	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Actin-like ATPases	8044470	3000092	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Chaperone J-domain	8033756	3000721	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	HSP70_1st	e2qwoA1	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: HSP70_1st	ECOD (1.6)
A	MreB_Mbl_C	e2qwoA2	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: MreB_Mbl_C	ECOD (1.6)
B	DnaJ	e2qwoB1	A: alpha bundles	X: Chaperone J-domain (From Topology)	H: Chaperone J-domain (From Topology)	T: Chaperone J-domain	F: DnaJ	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.420.40	Alpha Beta	2-Layer Sandwich	Nucleotidyltransferase	domain 5	CATH (4.3.0)
A	3.30.30.30	Alpha Beta	2-Layer Sandwich	Defensin A-like		CATH (4.3.0)
A	3.90.640.10	Alpha Beta	Alpha-Beta Complex	Actin	Chain A, domain 4	CATH (4.3.0)
B	1.10.287.110	Mainly Alpha	Orthogonal Bundle	Helix Hairpins	DnaJ domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00012	Hsp70 protein (HSP70)	Hsp70 protein	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Heat shock cognate 71 kDa protein	protein binding binding heat shock protein binding ATP-dependent protein folding chaperone protein folding chaperone ATP-dependent activity protein-macromolecule adaptor activity molecular adaptor activity adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding protein binding binding heat shock protein binding ATP-dependent protein folding chaperone protein folding chaperone ATP-dependent activity protein-macromolecule adaptor activity molecular adaptor activity adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding pyrophosphatase activity ATP hydrolysis activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity	regulation of cellular process negative regulation of biological process RNA metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process regulation of RNA metabolic process negative regulation of cellular process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of cellular process negative regulation of biological process RNA metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process regulation of RNA metabolic process negative regulation of cellular process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process negative regulation of macromolecule metabolic process regulation of biosynthetic process negative regulation of DNA-templated transcription regulation of biological process negative regulation of metabolic process biological regulation primary metabolic process regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of gene expression biosynthetic process negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process metabolic process cellular process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of DNA-templated transcription regulation of metabolic process negative regulation of RNA metabolic process protein refolding protein maturation protein folding protein metabolic process cellular component disassembly clathrin coat disassembly transport protein-containing complex organization protein-containing complex disassembly establishment of localization vesicle-mediated transport vesicle uncoating cellular component organization localization cellular component organization or biogenesis protein depolymerization mRNA processing mRNA metabolic process RNA processing RNA splicing protein localization establishment of protein localization cellular localization catabolic process nitrogen compound transport protein targeting to vacuole involved in autophagy protein targeting autophagy establishment of protein localization to vacuole protein targeting to lysosome involved in chaperone-mediated autophagy lysosomal transport macromolecule catabolic process chaperone-mediated autophagy protein targeting to lysosome cellular macromolecule localization establishment of localization in cell process utilizing autophagic mechanism protein targeting to vacuole intracellular transport protein localization to vacuole protein catabolic process protein localization to lysosome establishment of protein localization to organelle protein transport macromolecule localization protein localization to organelle vacuolar transport intracellular protein transport chaperone cofactor-dependent protein refolding chaperone-mediated protein folding 'de novo' protein folding 'de novo' post-translational protein folding import into cell synaptic vesicle uncoating synaptic vesicle cycle presynaptic endocytosis vesicle-mediated transport in synapse synaptic vesicle recycling synaptic vesicle endocytosis endocytosis	membrane-bounded organelle intracellular anatomical structure nucleus membrane-enclosed lumen intracellular membraneless organelle intracellular membrane-bounded organelle nucleolus intracellular organelle lumen intracellular organelle cellular anatomical structure organelle lumen organelle membraneless organelle nuclear lumen membrane-bounded organelle intracellular anatomical structure nucleus membrane-enclosed lumen intracellular membraneless organelle intracellular membrane-bounded organelle nucleolus intracellular organelle lumen intracellular organelle cellular anatomical structure organelle lumen organelle membraneless organelle nuclear lumen pigment granule intracellular vesicle melanosome cytoplasmic vesicle cytoplasm vesicle protein-containing complex Prp19 complex cytosol cell periphery plasma membrane membrane presynapse synapse cell junction vacuolar membrane lytic vacuole vacuole lytic vacuole membrane bounding membrane of organelle lysosomal membrane organelle membrane lysosome nuclear protein-containing complex spliceosomal complex ribonucleoprotein complex
B	Putative tyrosine-protein phosphatase auxilin	clathrin binding protein binding binding phosphoric ester hydrolase activity phosphoprotein phosphatase activity non-membrane spanning protein tyrosine phosphatase activity catalytic activity, acting on a protein hydrolase activity, acting on ester bonds protein tyrosine phosphatase activity phosphatase activity hydrolase activity catalytic activity protein domain specific binding histone modifying activity clathrin binding protein binding binding phosphoric ester hydrolase activity phosphoprotein phosphatase activity non-membrane spanning protein tyrosine phosphatase activity catalytic activity, acting on a protein hydrolase activity, acting on ester bonds protein tyrosine phosphatase activity phosphatase activity hydrolase activity catalytic activity protein domain specific binding histone modifying activity histone phosphatase activity histone H2AXY142 phosphatase activity heat shock protein binding clathrin heavy chain binding SH3 domain binding molecular adaptor activity protein tyrosine phosphatase activity, metal-dependent	synaptic vesicle recycling localization cellular localization transport establishment of localization in cell synaptic vesicle cycle cellular process establishment of localization vesicle-mediated transport vesicle-mediated transport in synapse cellular component disassembly clathrin coat disassembly protein-containing complex organization protein-containing complex disassembly synaptic vesicle recycling localization cellular localization transport establishment of localization in cell synaptic vesicle cycle cellular process establishment of localization vesicle-mediated transport vesicle-mediated transport in synapse cellular component disassembly clathrin coat disassembly protein-containing complex organization protein-containing complex disassembly vesicle uncoating cellular component organization cellular component organization or biogenesis protein depolymerization import into cell clathrin-dependent endocytosis receptor-mediated endocytosis endocytosis intracellular transport synaptic vesicle uncoating presynaptic endocytosis synaptic vesicle endocytosis regulation of biological process regulation of cellular process regulation of cellular component organization biological regulation regulation of clathrin coat assembly regulation of cellular component biogenesis clathrin coat assembly cellular component biogenesis regulation of protein-containing complex assembly cellular component assembly protein-containing complex assembly	membrane-bounded organelle cellular anatomical structure organelle vesicle intracellular anatomical structure intracellular vesicle intracellular membrane-bounded organelle intracellular organelle cytoplasmic vesicle clathrin-coated vesicle coated vesicle cytoplasm presynapse synapse membrane-bounded organelle cellular anatomical structure organelle vesicle intracellular anatomical structure intracellular vesicle intracellular membrane-bounded organelle intracellular organelle cytoplasmic vesicle clathrin-coated vesicle coated vesicle cytoplasm presynapse synapse cell junction postsynaptic density postsynapse neuron to neuron synapse postsynaptic specialization asymmetric synapse

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR018181	Heat shock protein 70, conserved site	Conserved Site
A	IPR043129	ATPase, nucleotide binding domain	Homologous Superfamily
A	IPR029048	Heat shock protein 70kD, C-terminal domain superfamily	Homologous Superfamily
A	IPR029047	Heat shock protein 70kD, peptide-binding domain superfamily	Homologous Superfamily
A	IPR013126	Heat shock protein 70 family	Family
B	IPR001623	DnaJ domain	Domain
B	IPR029023	Tensin-type phosphatase domain	Domain
B	IPR014020	Tensin phosphatase, C2 domain	Domain
B	IPR029021	Protein-tyrosine phosphatase-like	Homologous Superfamily
B	IPR036869	Chaperone J-domain superfamily	Homologous Superfamily
B	IPR000387	Tyrosine-specific protein phosphatases domain	Domain
B	IPR035892	C2 domain superfamily	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.