2OT0 | pdb_00002ot0

Fructose-1,6-bisphosphate aldolase from rabbit muscle in complex with a C-terminal peptide of Wiskott-Aldrich syndrome protein

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2ot0a_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Aldolase	Class I aldolase	Fructose-1,6-bisphosphate aldolase	rabbit (Oryctolagus cuniculus ) [TaxId: 9986 ],	SCOPe (2.08)
B	d2ot0b_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Aldolase	Class I aldolase	Fructose-1,6-bisphosphate aldolase	rabbit (Oryctolagus cuniculus ) [TaxId: 9986 ],	SCOPe (2.08)
C	d2ot0c_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Aldolase	Class I aldolase	Fructose-1,6-bisphosphate aldolase	rabbit (Oryctolagus cuniculus ) [TaxId: 9986 ],	SCOPe (2.08)
D	d2ot0d_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Aldolase	Class I aldolase	Fructose-1,6-bisphosphate aldolase	rabbit (Oryctolagus cuniculus ) [TaxId: 9986 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Aldolase	8035506	3000445	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Aldolase	8035506	3000445	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Aldolase	8035506	3000445	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Aldolase	8035506	3000445	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Glycolytic	e2ot0A1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Glycolytic	ECOD (1.6)
B	Glycolytic	e2ot0B1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Glycolytic	ECOD (1.6)
C	Glycolytic	e2ot0C1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Glycolytic	ECOD (1.6)
D	Glycolytic	e2ot0D1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Glycolytic	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.20.20.70	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Aldolase class I	CATH (4.3.0)
B	3.20.20.70	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Aldolase class I	CATH (4.3.0)
C	3.20.20.70	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Aldolase class I	CATH (4.3.0)
D	3.20.20.70	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Aldolase class I	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF00274	Fructose-bisphosphate aldolase class-I (Glycolytic)	Fructose-bisphosphate aldolase class-I		Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	Fructose-bisphosphate aldolase A	fructose-bisphosphate aldolase activity lyase activity catalytic activity carbon-carbon lyase activity aldehyde-lyase activity	protein-containing complex organization protein homotetramerization cellular component organization cellular component biogenesis cellular component assembly protein complex oligomerization protein tetramerization protein-containing complex assembly protein homooligomerization cellular process cellular component organization or biogenesis ribonucleotide catabolic process pyridine nucleotide catabolic process oxoacid metabolic process protein-containing complex organization protein homotetramerization cellular component organization cellular component biogenesis cellular component assembly protein complex oligomerization protein tetramerization protein-containing complex assembly protein homooligomerization cellular process cellular component organization or biogenesis ribonucleotide catabolic process pyridine nucleotide catabolic process oxoacid metabolic process carbohydrate catabolic process carbohydrate derivative metabolic process organophosphate catabolic process nucleoside phosphate catabolic process organic acid metabolic process nucleobase-containing compound metabolic process glycolytic process phosphate-containing compound metabolic process pyridine-containing compound metabolic process small molecule metabolic process purine ribonucleotide catabolic process phosphorus metabolic process pyridine nucleotide metabolic process nucleoside phosphate metabolic process nicotinamide nucleotide metabolic process pyruvate metabolic process purine nucleoside diphosphate metabolic process primary metabolic process purine-containing compound catabolic process nucleotide metabolic process nucleobase-containing compound catabolic process nucleoside diphosphate metabolic process purine nucleoside diphosphate catabolic process nucleoside diphosphate catabolic process metabolic process nucleoside triphosphate metabolic process monocarboxylic acid metabolic process purine ribonucleotide metabolic process pyridine-containing compound catabolic process ribose phosphate metabolic process catabolic process ATP metabolic process purine nucleotide metabolic process ribonucleotide metabolic process nucleobase-containing small molecule metabolic process purine ribonucleoside triphosphate metabolic process ribonucleoside diphosphate metabolic process ribonucleoside diphosphate catabolic process nucleotide catabolic process ribonucleoside triphosphate metabolic process purine nucleoside triphosphate metabolic process purine nucleotide catabolic process carbohydrate metabolic process carbohydrate derivative catabolic process ADP catabolic process carboxylic acid metabolic process generation of precursor metabolites and energy purine-containing compound metabolic process ADP metabolic process organophosphate metabolic process purine ribonucleoside diphosphate metabolic process purine ribonucleoside diphosphate catabolic process regulation of Arp2/3 complex-mediated actin nucleation regulation of cytoskeleton organization regulation of cellular component organization regulation of cellular process regulation of actin filament-based process negative regulation of biological process regulation of actin nucleation actin filament-based process negative regulation of cellular process negative regulation of supramolecular fiber organization negative regulation of actin nucleation negative regulation of Arp2/3 complex-mediated actin nucleation regulation of biological process regulation of actin filament organization actin cytoskeleton organization negative regulation of cellular component organization negative regulation of cytoskeleton organization actin filament organization biological regulation Arp2/3 complex-mediated actin nucleation regulation of organelle organization organelle organization negative regulation of organelle organization regulation of actin cytoskeleton organization regulation of supramolecular fiber organization actin nucleation supramolecular fiber organization cytoskeleton organization positive regulation of locomotion regulation of cell motility positive regulation of biological process positive regulation of cell migration regulation of locomotion positive regulation of cell motility regulation of cell migration cell migration cell motility locomotion positive regulation of cellular process	M band intracellular anatomical structure intracellular membraneless organelle sarcomere intracellular organelle cellular anatomical structure organelle membraneless organelle contractile muscle fiber A band cytoplasm supramolecular fiber myofibril supramolecular polymer M band intracellular anatomical structure intracellular membraneless organelle sarcomere intracellular organelle cellular anatomical structure organelle membraneless organelle contractile muscle fiber A band cytoplasm supramolecular fiber myofibril supramolecular polymer supramolecular complex I band
E, F, G, H	Wiskott-Aldrich syndrome protein C-terminal peptide	small GTPase binding protein binding binding enzyme binding GTPase binding phospholipase binding SH3 domain binding protein domain specific binding pyrophosphatase activity enzyme regulator activity GTPase activity nucleoside-triphosphatase regulator activity hydrolase activity GTPase regulator activity small GTPase binding protein binding binding enzyme binding GTPase binding phospholipase binding SH3 domain binding protein domain specific binding pyrophosphatase activity enzyme regulator activity GTPase activity nucleoside-triphosphatase regulator activity hydrolase activity GTPase regulator activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides molecular function regulator activity hydrolase activity, acting on acid anhydrides catalytic activity kinase binding protein kinase binding cytoskeletal protein binding actin binding identical protein binding	multicellular organismal process regulation of body fluid levels wound healing response to stimulus hemostasis biological regulation blood coagulation response to stress coagulation regulation of biological quality response to wounding cellular component organization cellular component biogenesis cellular component assembly multicellular organismal process regulation of body fluid levels wound healing response to stimulus hemostasis biological regulation blood coagulation response to stress coagulation regulation of biological quality response to wounding cellular component organization cellular component biogenesis cellular component assembly protein-containing complex organization protein-containing complex assembly cellular process cellular component organization or biogenesis regulation of cytoskeleton organization regulation of cellular process regulation of cellular component organization regulation of actin filament-based process regulation of actin filament bundle assembly actin filament bundle organization actin filament-based process stress fiber assembly regulation of biological process regulation of actin filament organization actin cytoskeleton organization actomyosin structure organization actin filament organization regulation of cellular component biogenesis regulation of organelle organization regulation of stress fiber assembly organelle organization contractile actin filament bundle assembly regulation of actomyosin structure organization regulation of actin cytoskeleton organization actin filament bundle assembly regulation of supramolecular fiber organization supramolecular fiber organization cytoskeleton organization immune response immune system process negative regulation of biological process negative regulation of cellular process negative regulation of supramolecular fiber organization negative regulation of stress fiber assembly negative regulation of cellular component organization negative regulation of cytoskeleton organization negative regulation of actin filament bundle assembly negative regulation of organelle organization protein polymerization actin polymerization or depolymerization actin filament polymerization developmental process epidermis development anatomical structure development tissue development negative regulation of locomotion regulation of cell motility regulation of locomotion negative regulation of cell motility cell motility locomotion defense response regulation of cellular component size regulation of actin polymerization or depolymerization regulation of actin filament length regulation of anatomical structure size T cell activation cell activation lymphocyte activation leukocyte activation cellular response to stimulus regulation of DNA recombination regulation of double-strand break repair regulation of cellular response to stress positive regulation of biological process positive regulation of double-strand break repair via homologous recombination regulation of primary metabolic process nucleobase-containing compound metabolic process positive regulation of response to stimulus positive regulation of metabolic process positive regulation of DNA repair cellular response to stress regulation of response to stress recombinational repair positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process double-strand break repair via homologous recombination positive regulation of cellular process DNA repair positive regulation of DNA recombination double-strand break repair positive regulation of nucleobase-containing compound metabolic process DNA recombination primary metabolic process DNA metabolic process regulation of macromolecule metabolic process nucleic acid metabolic process positive regulation of double-strand break repair regulation of DNA repair regulation of double-strand break repair via homologous recombination positive regulation of DNA metabolic process DNA damage response regulation of response to stimulus metabolic process macromolecule metabolic process regulation of metabolic process regulation of DNA metabolic process localization cellular localization transport establishment of localization in cell intracellular transport establishment of localization vesicle-mediated transport endosomal transport biological process involved in interspecies interaction between organisms response to other organism response to type II interferon response to cytokine defense response to symbiont response to external stimulus cellular response to type II interferon response to biotic stimulus response to peptide response to chemical defense response to other organism innate immune response cellular response to cytokine stimulus response to external biotic stimulus small GTPase-mediated signal transduction signaling Cdc42 protein signal transduction Rho protein signal transduction intracellular signal transduction cell communication intracellular signaling cassette signal transduction lamellipodium assembly plasma membrane bounded cell projection organization cell projection organization regulation of cell projection organization regulation of cell projection assembly plasma membrane bounded cell projection assembly regulation of plasma membrane bounded cell projection assembly regulation of lamellipodium assembly regulation of lamellipodium organization lamellipodium organization regulation of plasma membrane bounded cell projection organization cell projection assembly positive regulation of DNA-templated transcription RNA metabolic process positive regulation of macromolecule biosynthetic process RNA biosynthetic process nucleic acid biosynthetic process DNA-templated transcription regulation of RNA metabolic process transcription by RNA polymerase II positive regulation of biosynthetic process regulation of RNA biosynthetic process regulation of biosynthetic process regulation of transcription by RNA polymerase II positive regulation of transcription by RNA polymerase II nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process positive regulation of RNA metabolic process regulation of gene expression biosynthetic process positive regulation of RNA biosynthetic process regulation of macromolecule biosynthetic process regulation of DNA-templated transcription adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains lymphocyte mediated immunity regulation of immune effector process T cell mediated immunity T cell antigen processing and presentation antigen processing and presentation regulation of antigen processing and presentation regulation of adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains leukocyte mediated immunity regulation of immune system process regulation of T cell mediated immunity regulation of T cell antigen processing and presentation regulation of adaptive immune response adaptive immune response immune effector process regulation of leukocyte mediated immunity regulation of lymphocyte mediated immunity regulation of immune response actin filament-based movement	cell-cell junction cellular anatomical structure anchoring junction cell junction extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space vesicle extracellular membrane-bounded organelle intracellular organelle cell-cell junction cellular anatomical structure anchoring junction cell junction extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space vesicle extracellular membrane-bounded organelle intracellular organelle intracellular anatomical structure cytoskeleton membraneless organelle intracellular membraneless organelle actin cytoskeleton cytoplasm cytosol intracellular vesicle phagocytic vesicle intracellular membrane-bounded organelle cytoplasmic vesicle endocytic vesicle cell periphery plasma membrane membrane vesicle membrane organelle membrane polymeric cytoskeletal fiber actin filament supramolecular fiber supramolecular polymer supramolecular complex site of double-strand break site of DNA damage chromosome nucleus

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR000741	Fructose-bisphosphate aldolase, class-I	Family
A, B, C, D	IPR029768	Fructose-bisphosphate aldolase class-I active site	Conserved Site
A, B, C, D	IPR013785	Aldolase-type TIM barrel	Homologous Superfamily
E, F, G, H	IPR000095	CRIB domain	Domain
E, F, G, H	IPR000697	WH1/EVH1 domain	Domain
E, F, G, H	IPR036936	CRIB domain superfamily	Homologous Superfamily
E, F, G, H	IPR011993	PH-like domain superfamily	Homologous Superfamily
E, F, G, H	IPR003124	WH2 domain	Domain
E, F, G, H	IPR011026	Actin nucleation-promoting factor WAS, C-terminal	Homologous Superfamily
E, F, G, H	IPR033927	WASP family, EVH1 domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
E, F, G, H	Pharos: P42768	Wiskott-Aldrich syndrome thrombocytopenia 1 X-linked severe congenital neutropenia severe congenital neutropenia agranulocytosis lymphoma osteosarcoma cutaneous lupus erythematosus malignant mesothelioma psoriasis Thrombocytopenia, X-Linked, Intermittent leukopenia neutropenia

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.