2BGC

PrfA-G145S, a constitutive active mutant of the Transcriptional Regulator In L.monocytogenes

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2bgca1	All alpha proteins	DNA/RNA-binding 3-helical bundle	'Winged helix' DNA-binding domain	CAP C-terminal domain-like	automated matches	(Listeria monocytogenes EGD-e ) [TaxId: 169963 ],	SCOPe (2.08)
A	d2bgca2	All beta proteins	Double-stranded beta-helix	cAMP-binding domain-like	Listeriolysin regulatory protein PrfA, N-terminal domain	automated matches	(Listeria monocytogenes EGD-e ) [TaxId: 169963 ],	SCOPe (2.08)
B	d2bgcb1	All alpha proteins	DNA/RNA-binding 3-helical bundle	'Winged helix' DNA-binding domain	CAP C-terminal domain-like	automated matches	(Listeria monocytogenes EGD-e ) [TaxId: 169963 ],	SCOPe (2.08)
B	d2bgcb2	All beta proteins	Double-stranded beta-helix	cAMP-binding domain-like	Listeriolysin regulatory protein PrfA, N-terminal domain	automated matches	(Listeria monocytogenes EGD-e ) [TaxId: 169963 ],	SCOPe (2.08)
C [auth D]	d2bgcd1	All alpha proteins	DNA/RNA-binding 3-helical bundle	'Winged helix' DNA-binding domain	CAP C-terminal domain-like	automated matches	(Listeria monocytogenes EGD-e ) [TaxId: 169963 ],	SCOPe (2.08)
C [auth D]	d2bgcd2	All beta proteins	Double-stranded beta-helix	cAMP-binding domain-like	Listeriolysin regulatory protein PrfA, N-terminal domain	automated matches	(Listeria monocytogenes EGD-e ) [TaxId: 169963 ],	SCOPe (2.08)
D [auth E]	d2bgce1	All alpha proteins	DNA/RNA-binding 3-helical bundle	'Winged helix' DNA-binding domain	CAP C-terminal domain-like	automated matches	(Listeria monocytogenes EGD-e ) [TaxId: 169963 ],	SCOPe (2.08)
D [auth E]	d2bgce2	All beta proteins	Double-stranded beta-helix	cAMP-binding domain-like	Listeriolysin regulatory protein PrfA, N-terminal domain	automated matches	(Listeria monocytogenes EGD-e ) [TaxId: 169963 ],	SCOPe (2.08)
E [auth F]	d2bgcf1	All alpha proteins	DNA/RNA-binding 3-helical bundle	'Winged helix' DNA-binding domain	CAP C-terminal domain-like	automated matches	(Listeria monocytogenes EGD-e ) [TaxId: 169963 ],	SCOPe (2.08)
E [auth F]	d2bgcf2	All beta proteins	Double-stranded beta-helix	cAMP-binding domain-like	Listeriolysin regulatory protein PrfA, N-terminal domain	automated matches	(Listeria monocytogenes EGD-e ) [TaxId: 169963 ],	SCOPe (2.08)
F [auth G]	d2bgcg1	All alpha proteins	DNA/RNA-binding 3-helical bundle	'Winged helix' DNA-binding domain	CAP C-terminal domain-like	automated matches	(Listeria monocytogenes EGD-e ) [TaxId: 169963 ],	SCOPe (2.08)
F [auth G]	d2bgcg2	All beta proteins	Double-stranded beta-helix	cAMP-binding domain-like	Listeriolysin regulatory protein PrfA, N-terminal domain	automated matches	(Listeria monocytogenes EGD-e ) [TaxId: 169963 ],	SCOPe (2.08)
G [auth H]	d2bgch1	All alpha proteins	DNA/RNA-binding 3-helical bundle	'Winged helix' DNA-binding domain	CAP C-terminal domain-like	automated matches	(Listeria monocytogenes EGD-e ) [TaxId: 169963 ],	SCOPe (2.08)
G [auth H]	d2bgch2	All beta proteins	Double-stranded beta-helix	cAMP-binding domain-like	Listeriolysin regulatory protein PrfA, N-terminal domain	automated matches	(Listeria monocytogenes EGD-e ) [TaxId: 169963 ],	SCOPe (2.08)
H [auth I]	d2bgci1	All alpha proteins	DNA/RNA-binding 3-helical bundle	'Winged helix' DNA-binding domain	CAP C-terminal domain-like	automated matches	(Listeria monocytogenes EGD-e ) [TaxId: 169963 ],	SCOPe (2.08)
H [auth I]	d2bgci2	All beta proteins	Double-stranded beta-helix	cAMP-binding domain-like	Listeriolysin regulatory protein PrfA, N-terminal domain	automated matches	(Listeria monocytogenes EGD-e ) [TaxId: 169963 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Crp DNA-binding domain-like	8002768	4000157	SCOP2 (2022-06-29)
A	SCOP2 Family	cNMP-binding domain	8002769	4000272	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Winged helix DNA-binding domain	8017649	3000034	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	cAMP-binding domain-like	8017652	3000084	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	Winged helix DNA-binding domain	8017649	3000034	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	cAMP-binding domain-like	8017652	3000084	SCOP2B (2022-06-29)
C [auth D]	SCOP2B Superfamily	Winged helix DNA-binding domain	8017649	3000034	SCOP2B (2022-06-29)
C [auth D]	SCOP2B Superfamily	cAMP-binding domain-like	8017652	3000084	SCOP2B (2022-06-29)
D [auth E]	SCOP2B Superfamily	cAMP-binding domain-like	8017652	3000084	SCOP2B (2022-06-29)
D [auth E]	SCOP2B Superfamily	Winged helix DNA-binding domain	8017649	3000034	SCOP2B (2022-06-29)
E [auth F]	SCOP2B Superfamily	Winged helix DNA-binding domain	8017649	3000034	SCOP2B (2022-06-29)
E [auth F]	SCOP2B Superfamily	cAMP-binding domain-like	8017652	3000084	SCOP2B (2022-06-29)
F [auth G]	SCOP2B Superfamily	Winged helix DNA-binding domain	8017649	3000034	SCOP2B (2022-06-29)
F [auth G]	SCOP2B Superfamily	cAMP-binding domain-like	8017652	3000084	SCOP2B (2022-06-29)
G [auth H]	SCOP2B Superfamily	Winged helix DNA-binding domain	8017649	3000034	SCOP2B (2022-06-29)
G [auth H]	SCOP2B Superfamily	cAMP-binding domain-like	8017652	3000084	SCOP2B (2022-06-29)
H [auth I]	SCOP2B Superfamily	cAMP-binding domain-like	8017652	3000084	SCOP2B (2022-06-29)
H [auth I]	SCOP2B Superfamily	Winged helix DNA-binding domain	8017649	3000034	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	cNMP_binding	e2bgcA1	A: beta sandwiches	X: jelly-roll	H: Double-stranded beta-helix (From Topology)	T: Double-stranded beta-helix	F: cNMP_binding	ECOD (1.6)
A	HTH_Crp_2	e2bgcA2	A: alpha arrays	X: HTH	H: HTH	T: winged	F: HTH_Crp_2	ECOD (1.6)
B	cNMP_binding	e2bgcB1	A: beta sandwiches	X: jelly-roll	H: Double-stranded beta-helix (From Topology)	T: Double-stranded beta-helix	F: cNMP_binding	ECOD (1.6)
B	HTH_Crp_2	e2bgcB2	A: alpha arrays	X: HTH	H: HTH	T: winged	F: HTH_Crp_2	ECOD (1.6)
C [auth D]	cNMP_binding	e2bgcD1	A: beta sandwiches	X: jelly-roll	H: Double-stranded beta-helix (From Topology)	T: Double-stranded beta-helix	F: cNMP_binding	ECOD (1.6)
C [auth D]	HTH_Crp_2	e2bgcD2	A: alpha arrays	X: HTH	H: HTH	T: winged	F: HTH_Crp_2	ECOD (1.6)
D [auth E]	cNMP_binding	e2bgcE1	A: beta sandwiches	X: jelly-roll	H: Double-stranded beta-helix (From Topology)	T: Double-stranded beta-helix	F: cNMP_binding	ECOD (1.6)
D [auth E]	HTH_Crp_2	e2bgcE2	A: alpha arrays	X: HTH	H: HTH	T: winged	F: HTH_Crp_2	ECOD (1.6)
E [auth F]	cNMP_binding	e2bgcF1	A: beta sandwiches	X: jelly-roll	H: Double-stranded beta-helix (From Topology)	T: Double-stranded beta-helix	F: cNMP_binding	ECOD (1.6)
E [auth F]	HTH_Crp_2	e2bgcF2	A: alpha arrays	X: HTH	H: HTH	T: winged	F: HTH_Crp_2	ECOD (1.6)
F [auth G]	cNMP_binding	e2bgcG1	A: beta sandwiches	X: jelly-roll	H: Double-stranded beta-helix (From Topology)	T: Double-stranded beta-helix	F: cNMP_binding	ECOD (1.6)
F [auth G]	HTH_Crp_2	e2bgcG2	A: alpha arrays	X: HTH	H: HTH	T: winged	F: HTH_Crp_2	ECOD (1.6)
G [auth H]	cNMP_binding	e2bgcH1	A: beta sandwiches	X: jelly-roll	H: Double-stranded beta-helix (From Topology)	T: Double-stranded beta-helix	F: cNMP_binding	ECOD (1.6)
G [auth H]	HTH_Crp_2	e2bgcH2	A: alpha arrays	X: HTH	H: HTH	T: winged	F: HTH_Crp_2	ECOD (1.6)
H [auth I]	cNMP_binding	e2bgcI1	A: beta sandwiches	X: jelly-roll	H: Double-stranded beta-helix (From Topology)	T: Double-stranded beta-helix	F: cNMP_binding	ECOD (1.6)
H [auth I]	HTH_Crp_2	e2bgcI2	A: alpha arrays	X: HTH	H: HTH	T: winged	F: HTH_Crp_2	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.60.120.10	Mainly Beta	Sandwich	Jelly Rolls	Jelly Rolls	CATH (4.3.0)
A	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
B	2.60.120.10	Mainly Beta	Sandwich	Jelly Rolls	Jelly Rolls	CATH (4.3.0)
B	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
C [auth D]	2.60.120.10	Mainly Beta	Sandwich	Jelly Rolls	Jelly Rolls	CATH (4.3.0)
C [auth D]	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
D [auth E]	2.60.120.10	Mainly Beta	Sandwich	Jelly Rolls	Jelly Rolls	CATH (4.3.0)
D [auth E]	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
E [auth F]	2.60.120.10	Mainly Beta	Sandwich	Jelly Rolls	Jelly Rolls	CATH (4.3.0)
E [auth F]	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
F [auth G]	2.60.120.10	Mainly Beta	Sandwich	Jelly Rolls	Jelly Rolls	CATH (4.3.0)
F [auth G]	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
G [auth H]	2.60.120.10	Mainly Beta	Sandwich	Jelly Rolls	Jelly Rolls	CATH (4.3.0)
G [auth H]	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
H [auth I]	2.60.120.10	Mainly Beta	Sandwich	Jelly Rolls	Jelly Rolls	CATH (4.3.0)
H [auth I]	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C [auth D], D [auth E], E [auth F], A, B, C [auth D], D [auth E], E [auth F], F [auth G], G [auth H], H [auth I]	PRFA	DNA binding binding organic cyclic compound binding nucleic acid binding transcription regulator activity DNA-binding transcription factor activity	-	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C [auth D], D [auth E], E [auth F], A, B, C [auth D], D [auth E], E [auth F], F [auth G], G [auth H], H [auth I]	IPR018335	Transcription regulator HTH, Crp-type, conserved site	Conserved Site
A, B, C [auth D], D [auth E], E [auth F], A, B, C [auth D], D [auth E], E [auth F], F [auth G], G [auth H], H [auth I]	IPR018490	Cyclic nucleotide-binding domain superfamily	Homologous Superfamily
A, B, C [auth D], D [auth E], E [auth F], A, B, C [auth D], D [auth E], E [auth F], F [auth G], G [auth H], H [auth I]	IPR036388	Winged helix-like DNA-binding domain superfamily	Homologous Superfamily
A, B, C [auth D], D [auth E], E [auth F], A, B, C [auth D], D [auth E], E [auth F], F [auth G], G [auth H], H [auth I]	IPR036390	Winged helix DNA-binding domain superfamily	Homologous Superfamily
A, B, C [auth D], D [auth E], E [auth F], A, B, C [auth D], D [auth E], E [auth F], F [auth G], G [auth H], H [auth I]	IPR012318	Crp-type HTH domain	Domain
A, B, C [auth D], D [auth E], E [auth F], A, B, C [auth D], D [auth E], E [auth F], F [auth G], G [auth H], H [auth I]	IPR014710	RmlC-like jelly roll fold	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.