Annotations: 1SJ8

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1sj8a1	All alpha proteins	A middle domain of Talin 1	A middle domain of Talin 1	A middle domain of Talin 1	A middle domain of Talin 1	house mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
A	d1sj8a2	All alpha proteins	I/LWEQ domain	I/LWEQ domain	I/LWEQ domain	Talin 1	house mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	I/LWEQ domain	8027114	4002528	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	I/LWEQ domain-like	8039493	3001382	SCOP2 (2022-06-29)
A	SCOP2B Superfamily	Talin 1 middle domain-like	8039487	3001370	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	KOG4261_1st	e1sj8A1	A: alpha bundles	X: Four-helical up-and-down bundle	H: alpha-catenin-related	T: I/LWEQ domain (Pfam 01608)	F: KOG4261_1st	ECOD (1.6)
A	Talin_middle	e1sj8A2	A: alpha bundles	X: Four-helical up-and-down bundle	H: alpha-catenin-related	T: A middle domain of Talin 1	F: Talin_middle	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.20.1420.10	Mainly Alpha	Up-down Bundle	A middle domain of Talin 1	Talin, central domain	CATH (4.3.0)
A	1.20.120.230	Mainly Alpha	Up-down Bundle	Four Helix Bundle (Hemerythrin (Met), subunit A)	Alpha-catenin/vinculin-like	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF09141	Talin, middle domain (Talin_middle)	Talin, middle domain	Members of this family adopt a structure consisting of five alpha helices that fold into a bundle. They contain a Vinculin binding site (VBS) composed of a hydrophobic surface spanning five turns of helix four. Activation of the VBS causes subsequent ...	Members of this family adopt a structure consisting of five alpha helices that fold into a bundle. They contain a Vinculin binding site (VBS) composed of a hydrophobic surface spanning five turns of helix four. Activation of the VBS causes subsequent recruitment of Vinculin, which enables maturation of small integrin/talin complexes into more stable adhesions. Formation of the complex between VBS and Vinculin requires prior unfolding of this middle domain: once released from the talin hydrophobic core, the VBS helix is then available to induce the 'bundle conversion' conformational change within the vinculin head domain thereby displacing the intramolecular interaction with the vinculin tail, allowing vinculin to bind actin [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Talin 1	binding phospholipid binding lipid binding phosphatidylinositol binding cytoskeletal protein binding actin binding protein-containing complex binding protein binding actin filament binding LIM domain binding protein domain specific binding vinculin binding cell adhesion molecule binding integrin binding binding phospholipid binding lipid binding phosphatidylinositol binding cytoskeletal protein binding actin binding protein-containing complex binding protein binding actin filament binding LIM domain binding protein domain specific binding vinculin binding cell adhesion molecule binding integrin binding signaling receptor binding structural constituent of cytoskeleton structural molecule activity modified amino acid binding phosphatidylserine binding	cellular component organization cellular component biogenesis cellular component assembly protein-containing complex organization protein-containing complex assembly cellular process integrin activation cellular component organization or biogenesis cortical cytoskeleton organization microtubule cytoskeleton organization cytoplasmic microtubule organization cortical microtubule organization organelle organization microtubule-based process cellular component organization cellular component biogenesis cellular component assembly protein-containing complex organization protein-containing complex assembly cellular process integrin activation cellular component organization or biogenesis cortical cytoskeleton organization microtubule cytoskeleton organization cytoplasmic microtubule organization cortical microtubule organization organelle organization microtubule-based process supramolecular fiber organization cytoskeleton organization cellular response to stimulus regulation of biological process integrin-mediated signaling pathway regulation of cellular process cell surface receptor signaling pathway response to stimulus signaling biological regulation cell communication signal transduction cell junction organization cell junction assembly cell-substrate junction assembly cell-substrate junction organization actin cytoskeleton organization cortical actin cytoskeleton organization actin filament-based process cell adhesion	cellular anatomical structure anchoring junction cell junction focal adhesion cell-substrate junction ruffle cell leading edge plasma membrane membrane leading edge membrane plasma membrane bounded cell projection cell periphery cell projection ruffle membrane cellular anatomical structure anchoring junction cell junction focal adhesion cell-substrate junction ruffle cell leading edge plasma membrane membrane leading edge membrane plasma membrane bounded cell projection cell periphery cell projection ruffle membrane plasma membrane region cell projection membrane cell surface intracellular anatomical structure cytoplasm cell-cell junction adherens junction intracellular organelle cytoskeleton organelle membraneless organelle intracellular membraneless organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR000299	FERM domain	Domain
A	IPR002404	IRS-type PTB domain	Domain
A	IPR014352	FERM/acyl-CoA-binding protein superfamily	Homologous Superfamily
A	IPR019748	FERM central domain	Domain
A	IPR015009	Vinculin-binding site-containing domain	Domain
A	IPR032425	Talin, N-terminal F0 domain	Domain
A	IPR011993	PH-like domain superfamily	Homologous Superfamily
A	IPR035964	I/LWEQ domain superfamily	Homologous Superfamily
A	IPR015224	Talin, central	Domain
A	IPR054082	Talin, IBS2B domain	Domain
A	IPR019749	Band 4.1 domain	Domain
A	IPR035963	FERM superfamily, second domain	Homologous Superfamily
A	IPR036476	Talin, central domain superfamily	Homologous Superfamily
A	IPR036723	Alpha-catenin/vinculin-like superfamily	Homologous Superfamily
A	IPR002558	I/LWEQ domain	Domain
A	IPR019747	FERM conserved site	Conserved Site
A	IPR054060	Talin 1-like, rod-segment domain	Domain
A	IPR049108	Talin, R4 domain	Domain
A	IPR029071	Ubiquitin-like domain superfamily	Homologous Superfamily
A	IPR037438	Talin-1/2, rod-segment	Domain