1MUS | pdb_00001mus

crystal structure of Tn5 transposase complexed with resolved outside end DNA

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
C [auth A]	d1musa1	Alpha and beta proteins (a/b)	Ribonuclease H-like motif	Ribonuclease H-like	Transposase inhibitor (Tn5 transposase)	Transposase inhibitor (Tn5 transposase)	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
C [auth A]	d1musa2	Artifacts	Tags	Tags	Tags	C-terminal Tags	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
C [auth A]	SCOP2 Family	Transposase inhibitor (Tn5 transposase)	8031433	4000545	SCOP2 (2022-06-29)
C [auth A]	SCOP2 Superfamily	Ribonuclease H-like	8043811	3000143	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
C [auth A]	Dimer_Tnp_Tn5	e1musA1	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: Dimer_Tnp_Tn5	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
C [auth A]	1.10.246.40	Mainly Alpha	Orthogonal Bundle	Serum Albumin	Chain A, Domain 1	CATH (4.3.0)
C [auth A]	3.90.350.10	Alpha Beta	Alpha-Beta Complex	Transposase Inhibitor Protein From Tn5	Chain A, domain 1	CATH (4.3.0)
C [auth A]	1.10.740.10	Mainly Alpha	Orthogonal Bundle	Transferase Inhibitor Protein From Tn5	Chain A, domain2	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
C [auth A]	PF14706	Transposase DNA-binding (Tnp_DNA_bind)	Transposase DNA-binding	This domain occurs at the C-terminus of transposases including E. coli tnpA (Swiss:Q46731). TnpA encodes a transposase and an inhibitor protein, the inhibitor only differs from the transposase by the absence of the N-terminal 55 amino acids, which in ...	This domain occurs at the C-terminus of transposases including E. coli tnpA (Swiss:Q46731). TnpA encodes a transposase and an inhibitor protein, the inhibitor only differs from the transposase by the absence of the N-terminal 55 amino acids, which includes most of this domain [1]. This domain consists of alpha helices and turns, and functions as a DNA-binding domain [2].	Domain
C [auth A]	PF01609	Transposase DDE domain (DDE_Tnp_1)	Transposase DDE domain	Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The cat ...	Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction [3]. This family contains transposases for IS4 Swiss:P03835 [1], IS421 Swiss:P11901 [2], IS5377 Swiss:Q45620, IS427 [4], IS402 [5], IS1355 Swiss:O69604, IS5, which was original isolated in bacteriophage lambda [6].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
B [auth C]	DNA non-transferred strand	-	-	-
A [auth B]	DNA transferred strand	-	-	-
C [auth A]	Tn5 transposase	DNA binding binding nucleic acid binding endonuclease activity nuclease activity hydrolase activity, acting on ester bonds hydrolase activity catalytic activity cation binding ion binding metal ion binding small molecule binding transposase activity catalytic activity, acting on DNA DNA binding binding nucleic acid binding endonuclease activity nuclease activity hydrolase activity, acting on ester bonds hydrolase activity catalytic activity cation binding ion binding metal ion binding small molecule binding transposase activity catalytic activity, acting on DNA catalytic activity, acting on a nucleic acid	nucleobase-containing compound metabolic process DNA recombination cellular process metabolic process primary metabolic process DNA metabolic process macromolecule metabolic process transposition DNA transposition nucleic acid metabolic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
C [auth A]	IPR038215	Transposase, Tn5-like, N-terminal domain superfamily	Homologous Superfamily
C [auth A]	IPR014735	Transposase, Tn5-like, N-terminal	Domain
C [auth A]	IPR014737	Transposase, Tn5-like, C-terminal	Homologous Superfamily
C [auth A]	IPR054836	Transposase for transposon Tn5	Family
C [auth A]	IPR047768	Transposase for transposon Tn5-like	Family
C [auth A]	IPR002559	Transposase IS4-like domain	Domain
C [auth A]	IPR012337	Ribonuclease H-like superfamily	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.