1AWC

MOUSE GABP ALPHA/BETA DOMAIN BOUND TO DNA

Experimental Data Snapshot

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.15 Å
  • R-Value Free: 0.282 
  • R-Value Work: 0.211 

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This is version 1.3 of the entry. See complete history. 


Literature

The structure of GABPalpha/beta: an ETS domain- ankyrin repeat heterodimer bound to DNA.

Batchelor, A.H., Piper, D.E., de la Brousse, F.C., McKnight, S.L., Wolberger, C.

(1998) Science 279: 1037-1041

  • DOI: https://doi.org/10.1126/science.279.5353.1037
  • Primary Citation of Related Structures:  
    1AWC

  • PubMed Abstract: 

    GA-binding protein (GABP) is a transcriptional regulator composed of two structurally dissimilar subunits. The alpha subunit contains a DNA-binding domain that is a member of the ETS family, whereas the beta subunit contains a series of ankyrin repeats. The crystal structure of a ternary complex containing a GABPalpha/beta ETS domain-ankyrin repeat heterodimer bound to DNA was determined at 2. 15 angstrom resolution. The structure shows how an ETS domain protein can recruit a partner protein using both the ETS domain and a carboxyl-terminal extension and provides a view of an extensive protein-protein interface formed by a set of ankyrin repeats. The structure also reveals how the GABPalpha ETS domain binds to its core GGA DNA-recognition motif.

    • Organizational Affiliation: 

    Department of Biophysics and Biophysical Chemistry and the Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.


Macromolecules

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Entity ID: 3
MoleculeChains Sequence LengthOrganismDetailsImage
PROTEIN (GA BINDING PROTEIN ALPHA)C [auth A]110Mus musculusMutation(s): 0 
Gene Names: GABP ALPHA
UniProt & NIH Common Fund Data Resources
Find proteins for Q00422 (Mus musculus)
Explore Q00422 
Go to UniProtKB:  Q00422
IMPC:  MGI:95610
Entity Groups  
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UniProt GroupQ00422
Sequence Annotations
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Entity ID: 4
MoleculeChains Sequence LengthOrganismDetailsImage
PROTEIN (GA BINDING PROTEIN BETA 1)D [auth B]153Mus musculusMutation(s): 0 
Gene Names: GABP BETA 1
UniProt
Find proteins for Q00420 (Mus musculus)
Explore Q00420 
Go to UniProtKB:  Q00420
Entity Groups  
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UniProt GroupQ00420
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  • Reference Sequence

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Entity ID: 1
MoleculeChains LengthOrganismImage
DNA (5'-D(*AP*AP*(BRU)P*GP*AP*CP*CP*GP*GP*AP*AP*GP*TP*AP*(CBR)P*AP*CP*(CBR)P*GP*GP*A)-3')A [auth D]21N/A
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Entity ID: 2
MoleculeChains LengthOrganismImage
DNA (5'-D(*TP*TP*CP*CP*GP*GP*(BRU)P*GP*(BRU)P*AP*CP*TP*TP*CP*CP*GP*GP*TP*CP*AP*T)-3')B [auth E]21N/A
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: X-RAY DIFFRACTION
  • Resolution: 2.15 Å
  • R-Value Free: 0.282 
  • R-Value Work: 0.211 
  • Space Group: C 1 2 1
Unit Cell:
Length ( Å )Angle ( ˚ )
a = 201.6α = 90
b = 34.55β = 99.83
c = 59.26γ = 90
Software Package:
Software NamePurpose
MLPHAREphasing
X-PLORrefinement
DENZOdata reduction
SCALEPACKdata scaling

Structure Validation

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Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 1998-03-18
    Type: Initial release
  • Version 1.1: 2008-05-22
    Changes: Version format compliance
  • Version 1.2: 2011-07-13
    Changes: Version format compliance
  • Version 1.3: 2024-02-07
    Changes: Data collection, Database references, Derived calculations