Experiment: 7B5Y

 7B5Y

S. agalactiae BusR in complex with its busAB-promotor DNA


ELECTRON MICROSCOPY

Starting Model(s)

Initial Refinement Model(s)
TypeSourceAccession CodeDetails
experimental modelPDB 7B5T 
Sample
transcriptional repressor BusR bound to DNA
Sample Components
GntR family transcriptional regulator
BusR binding site in the busAB promotor DNA
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentLEICA EM GP
Cryogen NameETHANE
Sample Vitrification Details0.05% beta-octyl glycoside added prior to plunge freezing
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles20342
Reported Resolution (Å)7.1
Resolution MethodFSC 0.143 CUT-OFF
Other Details
Refinement Type
Symmetry TypePOINT
Point SymmetryC1
Map-Model Fitting and Refinement
Id1 (7B5T)
Refinement SpaceREAL
Refinement ProtocolRIGID BODY FIT
Refinement Target
Overall B Value
Fitting Procedure
Details
Data Acquisition
Detector TypeGATAN K2 SUMMIT (4k x 4k)
Electron Dose (electrons/Å**2)45
Imaging Experiment1
Date of Experiment
Temperature (Kelvin)
Microscope ModelFEI TITAN KRIOS
Minimum Defocus (nm)
Maximum Defocus (nm)
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS
Imaging ModeBRIGHT FIELD
Specimen Holder Model
Nominal Magnification
Calibrated Magnification
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
EM Software
TaskSoftware PackageVersion
PARTICLE SELECTIONcryoSPARCv2.15
PARTICLE SELECTIONRELIONv3.1
IMAGE ACQUISITIONEPU
CTF CORRECTIONcryoSPARC2.15
MODEL FITTINGCoot0.9.2
MODEL REFINEMENTPHENIX1.18.2-3874
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION370421CryoSparc blob-picker