Electron cryo-microscopy of human papillomavirus 16 and H16.V5 Fab fragments
ELECTRON MICROSCOPY
Sample
Mature HPV16 quasivirus capsid complexed with H16.V5 Fabs
Sample Components
Human papillomavirus 16
H16.V5 Fab
Specimen Preparation
Sample Aggregation State
PARTICLE
Vitrification Instrument
GATAN CRYOPLUNGE 3
Cryogen Name
ETHANE
Sample Vitrification Details
Blot for 0.7 seconds before plunging into liquid ethane (GATAN CRYOPLUNGE 3).
3D Reconstruction
Reconstruction Method
SINGLE PARTICLE
Number of Particles
2075
Reported Resolution (Å)
13.6
Resolution Method
FSC 0.5 CUT-OFF
Other Details
Semi-automatic particle selection was performed using e2boxer.py to obtain the particle coordinates, followed by particle boxing, linearization, norma ...
Semi-automatic particle selection was performed using e2boxer.py to obtain the particle coordinates, followed by particle boxing, linearization, normalization, and apodization of the images using Robem. Defocus and astigmatism values to perform contrast transfer function (CTF) correction were assessed using Robem for the extracted particles. The icosahedrally averaged reconstructions were initiated using a random model generated with setup_rmc and reached 14 A resolution estimated at a Fourier Shell Correlation (FSC) of 0.5. For the last step of refinement, the final maps were CTF-corrected using a B factor of 200 A2. (Single particle--Applied symmetry: I)
