Experiment: 2O3D

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	15N-13C-edited 3D NOESY	1mM SF2/ASF RRM2 U-15N,13C; 20mM phophate buffer Na; 50mM L-Arg; 50mM L-Glu; 5mM EDTA; 1mM DTT; 90% H2O, 10% D2O	90% H2O/10% D2O	20mM phosphate, 50mM NaCl, 50mM L-Arg, 50mM L-Glu	6.0	ambient	303
2	Backbone assignment experiments for TALOS	1mM SF2/ASF RRM2 U-15N,13C; 20mM phophate buffer Na; 50mM L-Arg; 50mM L-Glu; 5mM EDTA; 1mM DTT; 90% H2O, 10% D2O	90% H2O/10% D2O	20mM phosphate, 50mM NaCl, 50mM L-Arg, 50mM L-Glu	6.0	ambient	303

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	AVANCE	800

NMR Refinement
Method	Details	Software
simulated annealing		CNS

NMR Ensemble Information
Conformer Selection Criteria	structures with the lowest energy
Conformers Calculated Total Number	100
Conformers Submitted Total Number	20
Representative Model	1 (closest to the average)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	structure solution	CNS	1.1	Brunger et al
2	refinement	CNS	1.1	Brunger et al

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.