7E50 | pdb_00007e50

Crystal structure of human microplasmin in complex with kazal-type inhibitor AaTI

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
B	SCOP2B Superfamily	Trypsin-like serine proteases	8042267	3000114	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Kazal_1_1	e7e50A1	A: few secondary structure elements	X: Kazal-type serine protease inhibitors-like	H: Kazal-type serine protease inhibitors (From Topology)	T: Kazal-type serine protease inhibitors	F: Kazal_1_1	ECOD (1.6)
B	Trypsin_1	e7e50B1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: FMN-binding split barrel 2	F: Trypsin_1	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF07648	Kazal-type serine protease inhibitor domain (Kazal_2)	Kazal-type serine protease inhibitor domain	Usually indicative of serine protease inhibitors. However, kazal-like domains are also seen in the extracellular part of agrins, which are not known to be protease inhibitors. Kazal domains often occur in tandem arrays. Small alpha+beta fold containi ...	Usually indicative of serine protease inhibitors. However, kazal-like domains are also seen in the extracellular part of agrins, which are not known to be protease inhibitors. Kazal domains often occur in tandem arrays. Small alpha+beta fold containing three disulphides.	Domain
B	PF00089	Trypsin (Trypsin)	Trypsin		Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	AAEL006007-PA	-	-	cellular anatomical structure extracellular region extracellular space
B	Plasminogen	protein antigen binding protein binding binding antigen binding protein domain specific binding signaling receptor binding apolipoprotein binding enzyme binding protease binding peptidase activity catalytic activity, acting on a protein serine hydrolase activity serine-type peptidase activity hydrolase activity protein antigen binding protein binding binding antigen binding protein domain specific binding signaling receptor binding apolipoprotein binding enzyme binding protease binding peptidase activity catalytic activity, acting on a protein serine hydrolase activity serine-type peptidase activity hydrolase activity serine-type endopeptidase activity catalytic activity endopeptidase activity protein-folding chaperone binding kinase binding	multicellular organismal process regulation of body fluid levels wound healing response to stimulus hemostasis biological regulation blood coagulation response to stress coagulation regulation of biological quality response to wounding regulation of biological process regulation of cellular process regulation of cell adhesion multicellular organismal process regulation of body fluid levels wound healing response to stimulus hemostasis biological regulation blood coagulation response to stress coagulation regulation of biological quality response to wounding regulation of biological process regulation of cellular process regulation of cell adhesion negative regulation of biological process negative regulation of cell adhesion cell adhesion cell-cell adhesion negative regulation of cell-cell adhesion mediated by cadherin cell-cell adhesion mediated by cadherin negative regulation of cell-cell adhesion negative regulation of cellular process regulation of cell-cell adhesion cellular process regulation of cell-cell adhesion mediated by cadherin regulation of response to external stimulus regulation of response to wounding regulation of coagulation regulation of response to stress regulation of multicellular organismal process regulation of hemostasis negative regulation of response to stimulus negative regulation of wound healing regulation of blood coagulation negative regulation of blood coagulation response to external stimulus negative regulation of response to wounding negative regulation of hemostasis fibrinolysis negative regulation of coagulation regulation of response to stimulus negative regulation of response to external stimulus regulation of wound healing negative regulation of multicellular organismal process tissue remodeling cellular component organization cellular component disassembly extracellular matrix disassembly extracellular matrix organization cellular component organization or biogenesis extracellular structure organization external encapsulating structure organization biological process involved in interspecies interaction between organisms biological process involved in interaction with symbiont biological process involved in symbiotic interaction positive regulation of coagulation positive regulation of biological process positive regulation of blood coagulation positive regulation of response to stimulus positive regulation of response to external stimulus positive regulation of hemostasis positive regulation of response to wounding positive regulation of wound healing regulation of fibrinolysis positive regulation of multicellular organismal process negative regulation of fibrinolysis positive regulation of locomotion regulation of cell motility regulation of blood vessel endothelial cell migration endothelial cell migration positive regulation of cell migration positive regulation of blood vessel endothelial cell migration regulation of locomotion positive regulation of endothelial cell migration positive regulation of cell motility regulation of cell migration blood vessel endothelial cell migration regulation of endothelial cell migration cell migration cell motility locomotion positive regulation of cellular process negative regulation of cell population proliferation cell population proliferation regulation of cell population proliferation regulation of cell-substrate adhesion cell-substrate adhesion negative regulation of cell-substrate adhesion positive regulation of fibrinolysis proteolysis metabolic process primary metabolic process protein metabolic process macromolecule metabolic process	cell periphery cellular anatomical structure cell surface membrane plasma membrane external side of plasma membrane side of membrane extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space cell periphery cellular anatomical structure cell surface membrane plasma membrane external side of plasma membrane side of membrane extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space vesicle extracellular membrane-bounded organelle extracellular matrix external encapsulating structure collagen-containing extracellular matrix blood microparticle vesicle lumen intracellular anatomical structure intracellular vesicle membrane-enclosed lumen cytoplasmic vesicle lumen intracellular membrane-bounded organelle secretory granule lumen endomembrane system intracellular organelle lumen intracellular organelle cytoplasmic vesicle organelle lumen secretory granule secretory vesicle cytoplasm platelet alpha granule platelet alpha granule lumen

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR053265	Serine protease inhibitor	Family
A	IPR036058	Kazal domain superfamily	Homologous Superfamily
A	IPR002350	Kazal domain	Domain
B	IPR018056	Kringle, conserved site	Conserved Site
B	IPR003609	PAN/Apple domain	Domain
B	IPR018114	Serine proteases, trypsin family, histidine active site	Active Site
B	IPR013806	Kringle-like fold	Homologous Superfamily
B	IPR038178	Kringle superfamily	Homologous Superfamily
B	IPR009003	Peptidase S1, PA clan	Homologous Superfamily
B	IPR050759	Serine proteases and regulators with kringle domains	Family
B	IPR001314	Peptidase S1A, chymotrypsin family	Family
B	IPR033116	Serine proteases, trypsin family, serine active site	Active Site
B	IPR023317	Peptidase S1A, plasmin	Family
B	IPR001254	Serine proteases, trypsin domain	Domain
B	IPR043504	Peptidase S1, PA clan, chymotrypsin-like fold	Homologous Superfamily
B	IPR000001	Kringle	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
B	Pharos: P00747	thrombophilia hypertensive disorder hypoplasminogenemia heart disease Venous Thrombosis ligneous conjunctivitis pulmonary embolism temporal arteritis abdominal aortic aneurysm inborn carbohydrate metabolic disorder leukocyte disease aortic stenosis major depressive disorder cerebrovascular disorder myocardial infarction thrombophilia hypertensive disorder hypoplasminogenemia heart disease Venous Thrombosis ligneous conjunctivitis pulmonary embolism temporal arteritis abdominal aortic aneurysm inborn carbohydrate metabolic disorder leukocyte disease aortic stenosis major depressive disorder cerebrovascular disorder myocardial infarction peripheral arterial disease schizophrenia endometriosis acute kidney failure acute myocardial infarction atherosclerosis nephrosclerosis coronary artery disease drug-induced liver injury thrombotic disease hydrocephalus cancer metabolic syndrome X pancreatic ductal adenocarcinoma

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.