Annotations: 5MG3

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
F [auth C]	SCOP2B Superfamily	Galactose mutarotase-like	8101843	3000035	SCOP2B (2022-06-29)
F [auth C]	SCOP2B Superfamily	YidC transmembrane domain-like	8101842	3002720	SCOP2B (2022-06-29)
B [auth E]	SCOP2B Superfamily	Protein translocase subunit SecE-like	8053308	3002174	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
D	secD	e5mg3D2	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: FMN-binding split barrel	F: secD	ECOD (1.6)
D	SecD_SecF	e5mg3D1	A: alpha bundles	X: Multidrug efflux transporter AcrB transmembrane domain (From Topology)	H: Multidrug efflux transporter AcrB transmembrane domain (From Topology)	T: Multidrug efflux transporter AcrB transmembrane domain	F: SecD_SecF	ECOD (1.6)
D	secD_1	e5mg3D3	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: secD_1	ECOD (1.6)
F [auth C]	YidC_periplas	e5mg3C2	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: YidC_periplas	ECOD (1.6)
F [auth C]	60KD_IMP	e5mg3C1	A: alpha bundles	X: Membrane protein insertase YidC-related (From Homology)	H: Membrane protein insertase YidC-related	T: Membrane protein insertase YidC	F: 60KD_IMP	ECOD (1.6)
A [auth Y]	SecY	e5mg3Y1	A: alpha complex topology	X: Preprotein translocase SecY subunit (From Topology)	H: Preprotein translocase SecY subunit (From Topology)	T: Preprotein translocase SecY subunit	F: SecY	ECOD (1.6)
B [auth E]	SecE	e5mg3E1	A: extended segments	X: Preprotein translocase SecE subunit (From Topology)	H: Preprotein translocase SecE subunit (From Topology)	T: Preprotein translocase SecE subunit	F: SecE	ECOD (1.6)
C [auth G]	SecG	e5mg3G1	A: alpha bundles	X: Protein-export membrane protein secG (From Topology)	H: Protein-export membrane protein secG (From Topology)	T: Protein-export membrane protein secG	F: SecG	ECOD (1.6)
E [auth F]	SecD_SecF	e5mg3F1	A: alpha bundles	X: Multidrug efflux transporter AcrB transmembrane domain (From Topology)	H: Multidrug efflux transporter AcrB transmembrane domain (From Topology)	T: Multidrug efflux transporter AcrB transmembrane domain	F: SecD_SecF	ECOD (1.6)
E [auth F]	3a0501s07	e5mg3F2	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin domains in multidrug efflux transporter	T: Multidrug efflux transporter AcrB pore domain	F: 3a0501s07	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
D	PF21760	Protein translocase subunit SecDF, P1 domain, N-terminal (SecD_1st)	Protein translocase subunit SecDF, P1 domain, N-terminal	This domain is found in Protein translocase subunit SecDF from Thermus thermophilus, which belongs to the RND superfamily. SecDF shows 12 transmembrane (TM) regions and 6 periplasmic regions (P1-P6). This entry represents the N-terminal region of the ...	This domain is found in Protein translocase subunit SecDF from Thermus thermophilus, which belongs to the RND superfamily. SecDF shows 12 transmembrane (TM) regions and 6 periplasmic regions (P1-P6). This entry represents the N-terminal region of the base subdomain of the P1 domain that is located in the N- terminal half of the polypeptide, also known as SecD region. This domain folds into a a pseudo-symmetrical anti-parallel beta-sheet that rearranges to a beta-barrel architecture during ion transport. This domain, which covers the TM region [1-4], is related to Pfam:PF03176 and usually appears associated with Pfam:PF07549 and Pfam:PF02355.	Domain
D	PF22599	SecDF, P1 head subdomain (SecDF_P1_head)	SecDF, P1 head subdomain	This entry represents the head subdomain from P1 domain from SecDF proteins, which constitutes a critical element for proton transport [1-3]. P1 domain P1 binds an unfolded protein, and undergoes functionally important conformational changes. SecDF f ...	This entry represents the head subdomain from P1 domain from SecDF proteins, which constitutes a critical element for proton transport [1-3]. P1 domain P1 binds an unfolded protein, and undergoes functionally important conformational changes. SecDF functions as a membrane-integrated chaperone that mediates ATP-independent protein translocation.	Domain
D	PF02355	Protein export membrane protein SecD/SecF, C-terminal (SecD_SecF_C)	Protein export membrane protein SecD/SecF, C-terminal	This entry represents the C-terminal domain of various prokaryotic SecD and SecF protein export membrane proteins and their archaeal homologues. These SecD and SecF proteins are part of the multimeric protein export complex comprising SecA, D, E, F, ...	This entry represents the C-terminal domain of various prokaryotic SecD and SecF protein export membrane proteins and their archaeal homologues. These SecD and SecF proteins are part of the multimeric protein export complex comprising SecA, D, E, F, G, Y, and YajC[1]. SecD and SecF are required to maintain a proton motive force [2].	Domain
D	PF13721	SecD export protein N-terminal TM region (SecD-TM1)	SecD export protein N-terminal TM region	-	Family
F [auth C]	PF02096	60Kd inner membrane protein (60KD_IMP)	60Kd inner membrane protein	-	Family
F [auth C]	PF14849	YidC periplasmic domain (YidC_periplas)	YidC periplasmic domain	This is the periplasmic domain of YidC, a bacterial membrane protein which is required for the insertion and assembly of inner membrane proteins [1,2].	Domain
A [auth Y]	PF00344	SecY (SecY)	SecY	-	Family
B [auth E]	PF00584	SecE/Sec61-gamma subunits of protein translocation complex (SecE)	SecE/Sec61-gamma subunits of protein translocation complex	-	Family
E [auth F]	PF02355	Protein export membrane protein SecD/SecF, C-terminal (SecD_SecF_C)	Protein export membrane protein SecD/SecF, C-terminal	This entry represents the C-terminal domain of various prokaryotic SecD and SecF protein export membrane proteins and their archaeal homologues. These SecD and SecF proteins are part of the multimeric protein export complex comprising SecA, D, E, F, ...	This entry represents the C-terminal domain of various prokaryotic SecD and SecF protein export membrane proteins and their archaeal homologues. These SecD and SecF proteins are part of the multimeric protein export complex comprising SecA, D, E, F, G, Y, and YajC[1]. SecD and SecF are required to maintain a proton motive force [2].	Domain
E [auth F]	PF07549	SecD/SecF GG Motif (Sec_GG)	SecD/SecF GG Motif	-	Motif

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
D	Protein translocase subunit SecD	macromolecule transmembrane transporter activity active transmembrane transporter activity protein transporter activity primary active transmembrane transporter activity ATPase-coupled transmembrane transporter activity protein transmembrane transporter activity protein-transporting ATPase activity transporter activity transmembrane transporter activity ATP-dependent activity	protein localization localization establishment of protein localization cellular localization protein targeting cellular macromolecule localization macromolecule localization cellular process establishment of localization membrane organization establishment of protein localization to membrane localization within membrane protein localization to membrane cellular component organization protein localization localization establishment of protein localization cellular localization protein targeting cellular macromolecule localization macromolecule localization cellular process establishment of localization membrane organization establishment of protein localization to membrane localization within membrane protein localization to membrane cellular component organization protein insertion into membrane from inner side cellular component organization or biogenesis protein insertion into membrane nitrogen compound transport transport intracellular protein transmembrane transport intracellular transport transmembrane transport protein transmembrane transport protein transport establishment of localization in cell intracellular protein transport protein transport by the Sec complex	cellular anatomical structure membrane cell periphery plasma membrane protein-containing complex cell envelope Sec protein transport complex intracellular protein-containing complex
F [auth C]	Membrane protein insertase YidC	protein carrier chaperone membrane insertase activity molecular carrier activity	cellular component organization cellular component biogenesis cellular component assembly protein-containing complex organization protein-containing complex assembly cellular process cellular component organization or biogenesis protein folding biosynthetic process metabolic process primary metabolic process protein metabolic process macromolecule metabolic process gene expression cellular component organization cellular component biogenesis cellular component assembly protein-containing complex organization protein-containing complex assembly cellular process cellular component organization or biogenesis protein folding biosynthetic process metabolic process primary metabolic process protein metabolic process macromolecule metabolic process gene expression macromolecule biosynthetic process protein maturation membrane organization protein localization cellular localization establishment of protein localization establishment of protein localization to membrane localization within membrane protein localization to membrane establishment of localization localization cellular macromolecule localization macromolecule localization protein insertion into membrane from inner side protein insertion into membrane nitrogen compound transport transport protein transport by the Sec complex transmembrane transport protein transmembrane transport protein transport	cellular anatomical structure membrane cell periphery plasma membrane protein-containing complex cell envelope Sec protein transport complex intracellular protein-containing complex
A [auth Y]	Protein translocase subunit SecY	macromolecule transmembrane transporter activity protein transporter activity protein transmembrane transporter activity transporter activity transmembrane transporter activity signal sequence binding binding peptide binding	protein localization cellular localization establishment of protein localization nitrogen compound transport transport intracellular transport establishment of localization localization protein transport establishment of localization in cell cellular macromolecule localization macromolecule localization cellular process intracellular protein transport protein localization cellular localization establishment of protein localization nitrogen compound transport transport intracellular transport establishment of localization localization protein transport establishment of localization in cell cellular macromolecule localization macromolecule localization cellular process intracellular protein transport membrane organization establishment of protein localization to membrane localization within membrane protein localization to membrane cellular component organization protein insertion into membrane from inner side cellular component organization or biogenesis protein insertion into membrane intracellular protein transmembrane transport transmembrane transport protein transmembrane transport protein transport by the Sec complex protein targeting SRP-dependent cotranslational protein targeting to membrane cotranslational protein targeting to membrane establishment of protein localization to endoplasmic reticulum protein localization to endoplasmic reticulum protein targeting to membrane establishment of protein localization to organelle protein localization to organelle SRP-dependent cotranslational protein targeting to membrane, translocation protein targeting to ER	cellular anatomical structure membrane cell periphery plasma membrane protein-containing complex cell envelope Sec protein transport complex intracellular protein-containing complex
B [auth E]	Protein translocase subunit SecE	macromolecule transmembrane transporter activity protein transporter activity protein transmembrane transporter activity transporter activity transmembrane transporter activity	protein localization cellular localization establishment of protein localization nitrogen compound transport transport intracellular transport establishment of localization localization protein transport establishment of localization in cell cellular macromolecule localization macromolecule localization cellular process intracellular protein transport protein localization cellular localization establishment of protein localization nitrogen compound transport transport intracellular transport establishment of localization localization protein transport establishment of localization in cell cellular macromolecule localization macromolecule localization cellular process intracellular protein transport membrane organization establishment of protein localization to membrane localization within membrane protein localization to membrane cellular component organization protein insertion into membrane from inner side cellular component organization or biogenesis protein insertion into membrane secretion establishment of protein localization to extracellular region export from cell protein secretion protein localization to extracellular region secretion by cell intracellular protein transmembrane transport transmembrane transport protein transmembrane transport protein transport by the Sec complex protein targeting SRP-dependent cotranslational protein targeting to membrane cotranslational protein targeting to membrane establishment of protein localization to endoplasmic reticulum protein localization to endoplasmic reticulum protein targeting to membrane establishment of protein localization to organelle protein localization to organelle SRP-dependent cotranslational protein targeting to membrane, translocation protein targeting to ER	cellular anatomical structure membrane cell periphery plasma membrane protein-containing complex cell envelope Sec protein transport complex intracellular protein-containing complex
C [auth G]	Protein-export membrane protein SecG	macromolecule transmembrane transporter activity protein transporter activity protein transmembrane transporter activity transporter activity transmembrane transporter activity active transmembrane transporter activity primary active transmembrane transporter activity ATPase-coupled transmembrane transporter activity protein-transporting ATPase activity ATP-dependent activity	protein localization cellular localization establishment of protein localization nitrogen compound transport transport intracellular transport establishment of localization localization protein transport establishment of localization in cell cellular macromolecule localization macromolecule localization cellular process intracellular protein transport protein localization cellular localization establishment of protein localization nitrogen compound transport transport intracellular transport establishment of localization localization protein transport establishment of localization in cell cellular macromolecule localization macromolecule localization cellular process intracellular protein transport protein transport by the Sec complex transmembrane transport protein transmembrane transport protein targeting SRP-dependent cotranslational protein targeting to membrane cotranslational protein targeting to membrane establishment of protein localization to endoplasmic reticulum protein localization to endoplasmic reticulum establishment of protein localization to membrane intracellular protein transmembrane transport localization within membrane protein localization to membrane protein targeting to membrane establishment of protein localization to organelle protein localization to organelle SRP-dependent cotranslational protein targeting to membrane, translocation protein targeting to ER membrane organization cellular component organization protein insertion into membrane from inner side cellular component organization or biogenesis protein insertion into membrane secretion establishment of protein localization to extracellular region export from cell protein secretion protein localization to extracellular region secretion by cell	cell periphery cellular anatomical structure plasma membrane membrane protein-containing complex cell envelope Sec protein transport complex intracellular protein-containing complex
E [auth F]	Protein translocase subunit SecF	macromolecule transmembrane transporter activity active transmembrane transporter activity protein transporter activity primary active transmembrane transporter activity ATPase-coupled transmembrane transporter activity protein transmembrane transporter activity protein-transporting ATPase activity transporter activity transmembrane transporter activity ATP-dependent activity	protein localization localization establishment of protein localization cellular localization protein targeting cellular macromolecule localization macromolecule localization cellular process establishment of localization membrane organization establishment of protein localization to membrane localization within membrane protein localization to membrane cellular component organization protein localization localization establishment of protein localization cellular localization protein targeting cellular macromolecule localization macromolecule localization cellular process establishment of localization membrane organization establishment of protein localization to membrane localization within membrane protein localization to membrane cellular component organization protein insertion into membrane from inner side cellular component organization or biogenesis protein insertion into membrane nitrogen compound transport transport intracellular protein transmembrane transport intracellular transport transmembrane transport protein transmembrane transport protein transport establishment of localization in cell intracellular protein transport protein transport by the Sec complex	cellular anatomical structure membrane cell periphery plasma membrane protein-containing complex cell envelope Sec protein transport complex intracellular protein-containing complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
D	IPR054384	SecDF, P1 head subdomain	Domain
D	IPR022646	Protein-export membrane protein SecD/SecF/SecDF, conserved site	Conserved Site
D	IPR048631	Protein translocase subunit SecDF, P1 domain, N-terminal	Domain
D	IPR022813	Protein-export membrane protein SecD/SecF, archaeal and bacterial	Family
D	IPR048634	Protein export membrane protein SecD/SecF, C-terminal	Domain
D	IPR005791	Protein translocase subunit SecD	Family
D	IPR027398	SecD export protein N-terminal TM domain	Domain
F [auth C]	IPR047196	Membrane insertase YidC/ALB, C-terminal	Domain
F [auth C]	IPR019998	Membrane insertase YidC	Family
F [auth C]	IPR001708	Membrane insertase YidC/ALB3/OXA1/COX18	Family
F [auth C]	IPR028053	Membrane insertase YidC, N-terminal	Domain
F [auth C]	IPR028055	Membrane insertase YidC/Oxa/ALB, C-terminal	Domain
F [auth C]	IPR038221	YidC, periplasmic domain superfamily	Homologous Superfamily
A [auth Y]	IPR002208	SecY/SEC61-alpha family	Family
A [auth Y]	IPR030659	SecY conserved site	Conserved Site
A [auth Y]	IPR023201	SecY domain superfamily	Homologous Superfamily
A [auth Y]	IPR026593	Protein translocase subunit SecY	Family
B [auth E]	IPR005807	SecE subunit of protein translocation complex, bacterial-like	Family
B [auth E]	IPR038379	SecE superfamily	Homologous Superfamily
B [auth E]	IPR001901	Protein translocase complex, SecE/Sec61-gamma subunit	Family
C [auth G]	IPR004692	Preprotein translocase SecG subunit	Family
E [auth F]	IPR005665	Protein-export membrane protein SecF, bacterial	Family
E [auth F]	IPR022646	Protein-export membrane protein SecD/SecF/SecDF, conserved site	Conserved Site
E [auth F]	IPR022645	Protein-export membrane protein SecD/SecF, bacterial	Family
E [auth F]	IPR022813	Protein-export membrane protein SecD/SecF, archaeal and bacterial	Family
E [auth F]	IPR048634	Protein export membrane protein SecD/SecF, C-terminal	Domain

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A [auth Y], B [auth E], C [auth G], D, E [auth F] A [auth Y], B [auth E], C [auth G], D, E [auth F], F [auth C]	OPM	Transmembrane	Alpha-helical polytopic	General secretory pathway (Sec)	Protein translocase

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A [auth Y], B [auth E], C [auth G], D, E [auth F] A [auth Y], B [auth E], C [auth G], D, E [auth F], F [auth C]	PDBTM	Annotated as membrane protein by PDBTM

Membrane Protein Annotation: MemProtMD MemProtMD Database Homepage

Chains	External Link	Comment
A [auth Y], B [auth E], C [auth G], D, E [auth F] A [auth Y], B [auth E], C [auth G], D, E [auth F], F [auth C]	MemProtMD	Annotated as membrane protein by MemProtMD