4USJ

N-acetylglutamate kinase from Arabidopsis thaliana in complex with PII from Chlamydomonas reinhardtii

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Structure Motif: Primary M-CSA Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d4usja_	Alpha and beta proteins (a/b)	Carbamate kinase-like	Carbamate kinase-like	automated matches	automated matches	THALE CRESS (Arabidopsis thaliana ) [TaxId: 3702 ],	SCOPe (2.08)
B	d4usjb_	Alpha and beta proteins (a/b)	Carbamate kinase-like	Carbamate kinase-like	automated matches	automated matches	THALE CRESS (Arabidopsis thaliana ) [TaxId: 3702 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Carbamate kinase-like	8084036	3001310	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Carbamate kinase-like	8084036	3001310	SCOP2B (2022-06-29)
C	SCOP2 Family	Prokaryotic signal transducing protein	8074430	4001256	SCOP2 (2022-06-29)
C	SCOP2 Superfamily	GlnB-like	8074431	3001165	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	AA_kinase	e4usjA1	A: a/b three-layered sandwiches	X: Carbamate kinase-like (From Topology)	H: Carbamate kinase-like (From Topology)	T: Carbamate kinase-like	F: AA_kinase	ECOD (1.6)
B	AA_kinase	e4usjB1	A: a/b three-layered sandwiches	X: Carbamate kinase-like (From Topology)	H: Carbamate kinase-like (From Topology)	T: Carbamate kinase-like	F: AA_kinase	ECOD (1.6)
C	P-II	e4usjC1	A: a+b two layers	X: Alpha-beta plaits	H: GlnB-like (From Topology)	T: GlnB-like	F: P-II	ECOD (1.6)
D	P-II	e4usjD1	A: a+b two layers	X: Alpha-beta plaits	H: GlnB-like (From Topology)	T: GlnB-like	F: P-II	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.1160.10	Alpha Beta	3-Layer(aba) Sandwich	Carbamate kinase	Acetylglutamate kinase-like	CATH (4.3.0)
B	3.40.1160.10	Alpha Beta	3-Layer(aba) Sandwich	Carbamate kinase	Acetylglutamate kinase-like	CATH (4.3.0)
C	3.30.70.120	Alpha Beta	2-Layer Sandwich	Alpha-Beta Plaits		CATH (4.3.0)
D	3.30.70.120	Alpha Beta	2-Layer Sandwich	Alpha-Beta Plaits		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF00696	Amino acid kinase family (AA_kinase)	Amino acid kinase family	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	ACETYLGLUTAMATE KINASE, CHLOROPLASTIC	kinase activity acetylglutamate kinase activity phosphotransferase activity, carboxyl group as acceptor transferase activity catalytic activity transferase activity, transferring phosphorus-containing groups binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding kinase activity acetylglutamate kinase activity phosphotransferase activity, carboxyl group as acceptor transferase activity catalytic activity transferase activity, transferring phosphorus-containing groups binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding cation binding organic acid binding amino acid binding carboxylic acid binding arginine binding	phosphorylation phosphate-containing compound metabolic process metabolic process cellular process phosphorus metabolic process ornithine metabolic process oxoacid metabolic process small molecule biosynthetic process carboxylic acid metabolic process amino acid metabolic process primary metabolic process organic acid metabolic process organic acid biosynthetic process alpha-amino acid metabolic process phosphorylation phosphate-containing compound metabolic process metabolic process cellular process phosphorus metabolic process ornithine metabolic process oxoacid metabolic process small molecule biosynthetic process carboxylic acid metabolic process amino acid metabolic process primary metabolic process organic acid metabolic process organic acid biosynthetic process alpha-amino acid metabolic process carboxylic acid biosynthetic process arginine biosynthetic process via ornithine arginine metabolic process non-proteinogenic amino acid metabolic process amino acid biosynthetic process biosynthetic process small molecule metabolic process alpha-amino acid biosynthetic process	membrane-bounded organelle intracellular anatomical structure plastid intracellular membrane-bounded organelle intracellular organelle cellular anatomical structure plastid stroma organelle chloroplast stroma cytoplasm chloroplast cytosol intracellular membraneless organelle plastid thylakoid membrane-bounded organelle intracellular anatomical structure plastid intracellular membrane-bounded organelle intracellular organelle cellular anatomical structure plastid stroma organelle chloroplast stroma cytoplasm chloroplast cytosol intracellular membraneless organelle plastid thylakoid chloroplast thylakoid membraneless organelle thylakoid
C, D	NITROGEN REGULATORY PROTEIN PII	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR004662	Acetylglutamate kinase family	Family
A, B	IPR001048	Aspartate/glutamate/uridylate kinase	Domain
A, B	IPR037528	Acetylglutamate kinase ArgB	Family
A, B	IPR001057	Glutamate/acetylglutamate kinase	Family
A, B	IPR041727	N-Acetyl-L-glutamate kinase, cyclic	Family
A, B	IPR036393	Acetylglutamate kinase-like superfamily	Homologous Superfamily

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	acetylglutamate kinase M-CSA #870	Catalyses the ATP-dependent phosphorylation of N-acetyl-L-glutamate (NAG). NAG fulfils distinct biological roles in lower and higher organisms. In prokaryotes, lower eukaryotes and plants it is the first intermediate in the biosynthesis of arginine, whereas in ureotelic (excreting nitrogen mostly in the form of urea) vertebrates, it is an essential allosteric cofactor for carbamoyl phosphate synthetase I (CPSI), the first enzyme of the urea cycle. This enzyme is present only in microorganisms and plants but absent in mammals, and as a result it is an attractive target for antimicrobial or biocidal development.	Defined by 7 residues: LYS:A-62 [auth A-41]GLY:A-65 [auth A-44]GLY:A-97 [auth A-76]ARG:A-119 [auth A-98]ASP:A-217 [auth A-196]ASP:A-237 [auth A-216]LYS:A-276 [auth A-255] \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 2.7.2.8 (PDB Primary Data) Search M-CSA Motif + EC 2.7.2.8

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.