4IJE

Crystal structure of the Zaire ebolavirus VP35 interferon inhibitory domain R312A/K319A/R322A mutant

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d4ijea1	Alpha and beta proteins (a+b)	Filoviridae VP35-like	Filoviridae VP35-like	Filoviridae VP35	Filoviridae VP35	ZEBOV (Ebola virus - Mayinga, Zaire, 1976 ) [TaxId: 128952 ],	SCOPe (2.08)
A	d4ijea2	Artifacts	Tags	Tags	Tags	N-terminal Tags	ZEBOV (Ebola virus - Mayinga, Zaire, 1976 ) [TaxId: 128952 ],	SCOPe (2.08)
B	d4ijeb1	Alpha and beta proteins (a+b)	Filoviridae VP35-like	Filoviridae VP35-like	Filoviridae VP35	Filoviridae VP35	ZEBOV (Ebola virus - Mayinga, Zaire, 1976 ) [TaxId: 128952 ],	SCOPe (2.08)
B	d4ijeb2	Artifacts	Tags	Tags	Tags	N-terminal Tags	ZEBOV (Ebola virus - Mayinga, Zaire, 1976 ) [TaxId: 128952 ],	SCOPe (2.08)
C	d4ijec1	Alpha and beta proteins (a+b)	Filoviridae VP35-like	Filoviridae VP35-like	Filoviridae VP35	Filoviridae VP35	ZEBOV (Ebola virus - Mayinga, Zaire, 1976 ) [TaxId: 128952 ],	SCOPe (2.08)
C	d4ijec2	Artifacts	Tags	Tags	Tags	N-terminal Tags	ZEBOV (Ebola virus - Mayinga, Zaire, 1976 ) [TaxId: 128952 ],	SCOPe (2.08)
D	d4ijed1	Alpha and beta proteins (a+b)	Filoviridae VP35-like	Filoviridae VP35-like	Filoviridae VP35	Filoviridae VP35	ZEBOV (Ebola virus - Mayinga, Zaire, 1976 ) [TaxId: 128952 ],	SCOPe (2.08)
D	d4ijed2	Artifacts	Tags	Tags	Tags	N-terminal Tags	ZEBOV (Ebola virus - Mayinga, Zaire, 1976 ) [TaxId: 128952 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Ebola VP35 IID-like	8092332	3002590	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Ebola VP35 IID-like	8092332	3002590	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Ebola VP35 IID-like	8092332	3002590	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Ebola VP35 IID-like	8092332	3002590	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Filo_VP35	e4ijeA1	A: a+b complex topology	X: Polymerase cofactor VP35 C-terminal RNA binding domain (From Topology)	H: Polymerase cofactor VP35 C-terminal RNA binding domain (From Topology)	T: Polymerase cofactor VP35 C-terminal RNA binding domain	F: Filo_VP35	ECOD (1.6)
B	Filo_VP35	e4ijeB1	A: a+b complex topology	X: Polymerase cofactor VP35 C-terminal RNA binding domain (From Topology)	H: Polymerase cofactor VP35 C-terminal RNA binding domain (From Topology)	T: Polymerase cofactor VP35 C-terminal RNA binding domain	F: Filo_VP35	ECOD (1.6)
C	Filo_VP35	e4ijeC1	A: a+b complex topology	X: Polymerase cofactor VP35 C-terminal RNA binding domain (From Topology)	H: Polymerase cofactor VP35 C-terminal RNA binding domain (From Topology)	T: Polymerase cofactor VP35 C-terminal RNA binding domain	F: Filo_VP35	ECOD (1.6)
D	Filo_VP35	e4ijeD1	A: a+b complex topology	X: Polymerase cofactor VP35 C-terminal RNA binding domain (From Topology)	H: Polymerase cofactor VP35 C-terminal RNA binding domain (From Topology)	T: Polymerase cofactor VP35 C-terminal RNA binding domain	F: Filo_VP35	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.10.8.950	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
A	2.10.10.70	Mainly Beta	Ribbon	Seminal Fluid Protein PDC-109 (Domain B)	Filoviridae VP35, C-terminal inhibitory domain, beta-sheet subdomain	CATH (4.3.0)
B	1.10.8.950	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
B	2.10.10.70	Mainly Beta	Ribbon	Seminal Fluid Protein PDC-109 (Domain B)	Filoviridae VP35, C-terminal inhibitory domain, beta-sheet subdomain	CATH (4.3.0)
C	1.10.8.950	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
C	2.10.10.70	Mainly Beta	Ribbon	Seminal Fluid Protein PDC-109 (Domain B)	Filoviridae VP35, C-terminal inhibitory domain, beta-sheet subdomain	CATH (4.3.0)
D	1.10.8.950	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
D	2.10.10.70	Mainly Beta	Ribbon	Seminal Fluid Protein PDC-109 (Domain B)	Filoviridae VP35, C-terminal inhibitory domain, beta-sheet subdomain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF02097	Filoviridae VP35 (Filo_VP35)	Filoviridae VP35	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	Polymerase cofactor VP35	molecular sequestering activity RNA binding binding organic cyclic compound binding nucleic acid binding	viral gene expression viral process viral transcription regulation of protein sumoylation regulation of cellular process regulation of protein modification process positive regulation of protein metabolic process peptidyl-lysine modification positive regulation of biological process positive regulation of post-translational protein modification regulation of primary metabolic process positive regulation of metabolic process positive regulation of protein modification process peptidyl-amino acid modification viral gene expression viral process viral transcription regulation of protein sumoylation regulation of cellular process regulation of protein modification process positive regulation of protein metabolic process peptidyl-lysine modification positive regulation of biological process positive regulation of post-translational protein modification regulation of primary metabolic process positive regulation of metabolic process positive regulation of protein modification process peptidyl-amino acid modification positive regulation of macromolecule metabolic process protein metabolic process regulation of protein modification by small protein conjugation or removal protein modification process positive regulation of cellular process positive regulation of protein modification by small protein conjugation or removal regulation of biological process regulation of post-translational protein modification regulation of protein metabolic process biological regulation primary metabolic process regulation of macromolecule metabolic process protein sumoylation macromolecule modification metabolic process cellular process macromolecule metabolic process positive regulation of protein sumoylation post-translational protein modification regulation of metabolic process protein modification by small protein conjugation protein modification by small protein conjugation or removal symbiont-mediated perturbation of host cellular process biological process involved in interspecies interaction between organisms response to stimulus symbiont-mediated suppression of host pathogen-associated molecular pattern receptor signaling pathway response to biotic stimulus biological process involved in symbiotic interaction symbiont-mediated suppression of host immune response symbiont-mediated suppression of host innate immune response symbiont-mediated perturbation of host process symbiont-mediated suppression of host defenses symbiont-mediated perturbation of host innate immune response response to host response to external biotic stimulus symbiont-mediated perturbation of host signal transduction pathway modulation of process of another organism symbiont-mediated perturbation of host defense response response to host defenses response to other organism symbiont-mediated perturbation of host immune response symbiont-mediated perturbation of host defenses response to defenses of other organism response to external stimulus biological process involved in interaction with host symbiont-mediated suppression of host signal transduction pathway symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IKBKE activity symbiont-mediated suppression of cytoplasmic pattern recognition receptor signaling pathway symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF7 activity negative regulation of biological process positive regulation of macromolecule biosynthetic process regulation of miRNA-mediated gene silencing post-transcriptional regulation of gene expression negative regulation of post-transcriptional gene silencing by regulatory ncRNA regulation of gene silencing by regulatory ncRNA positive regulation of biosynthetic process negative regulation of cellular process regulation of post-transcriptional gene silencing by regulatory ncRNA negative regulation of macromolecule metabolic process regulation of biosynthetic process negative regulation of metabolic process negative regulation of gene silencing by regulatory ncRNA gene expression macromolecule biosynthetic process regulatory ncRNA-mediated post-transcriptional gene silencing regulatory ncRNA-mediated gene silencing miRNA-mediated post-transcriptional gene silencing regulation of gene expression negative regulation of gene expression biosynthetic process regulation of post-transcriptional gene silencing negative regulation of biosynthetic process positive regulation of gene expression negative regulation of miRNA-mediated gene silencing negative regulation of macromolecule biosynthetic process post-transcriptional gene silencing regulation of macromolecule biosynthetic process negative regulation of post-transcriptional gene silencing symbiont-mediated suppression of host RNAi-mediated antiviral immune response symbiont-mediated suppression of host toll-like receptor signaling pathway viral genome replication viral life cycle multicellular organismal process modulation by virus of host process symbiont-mediated suppression of host gene expression cytokine production type I interferon production symbiont-mediated perturbation of host gene expression negative regulation of multicellular organismal process virus-mediated perturbation of host defense response perturbation by virus of host immune response suppression by virus of host type I interferon production negative regulation of type I interferon production regulation of cytokine production regulation of multicellular organismal process modulation by virus of host cellular process suppression by virus of host cytokine production negative regulation of cytokine production modulation by virus of host cytokine production symbiont-mediated suppression of host cytokine production regulation of type I interferon production symbiont-mediated suppression of host antigen processing and presentation of peptide antigen via MHC class II symbiont-mediated suppression of host antigen processing and presentation symbiont-mediated suppression of host adaptive immune response symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity	viral nucleocapsid viral capsid virion component host cellular component host intracellular part cellular anatomical structure host cell cytoplasm host cell part host intracellular region

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR031163	Filoviruses VP35 interferon inhibitory domain	Domain
A, B, C, D	IPR002953	Filoviridae VP35 protein	Family
A, B, C, D	IPR043061	Filoviruses VP35 interferon inhibitory domain, beta-sheet subdomain	Homologous Superfamily
A, B, C, D	IPR043060	Filoviruses VP35 interferon inhibitory domain, helical subdomain	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.