2Z2S | pdb_00002z2s

Crystal Structure of Rhodobacter sphaeroides SigE in complex with the anti-sigma ChrR

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Sigma70_r4_2	e2z2sA4	A: alpha arrays	X: HTH	H: HTH	T: tri-helical	F: Sigma70_r4_2	ECOD (1.6)
A	Sigma70_r2	e2z2sA3	A: alpha complex topology	X: Sigma2 domain-like	H: Sigma2 domain of RNA polymerase sigma factors (From Topology)	T: Sigma2 domain of RNA polymerase sigma factors	F: Sigma70_r2	ECOD (1.6)
C	Sigma70_r4_2	e2z2sC4	A: alpha arrays	X: HTH	H: HTH	T: tri-helical	F: Sigma70_r4_2	ECOD (1.6)
C	Sigma70_r2	e2z2sC3	A: alpha complex topology	X: Sigma2 domain-like	H: Sigma2 domain of RNA polymerase sigma factors (From Topology)	T: Sigma2 domain of RNA polymerase sigma factors	F: Sigma70_r2	ECOD (1.6)
E	Sigma70_r4_2	e2z2sE6	A: alpha arrays	X: HTH	H: HTH	T: tri-helical	F: Sigma70_r4_2	ECOD (1.6)
E	Sigma70_r2	e2z2sE5	A: alpha complex topology	X: Sigma2 domain-like	H: Sigma2 domain of RNA polymerase sigma factors (From Topology)	T: Sigma2 domain of RNA polymerase sigma factors	F: Sigma70_r2	ECOD (1.6)
G	Sigma70_r4_2	e2z2sG4	A: alpha arrays	X: HTH	H: HTH	T: tri-helical	F: Sigma70_r4_2	ECOD (1.6)
G	Sigma70_r2	e2z2sG3	A: alpha complex topology	X: Sigma2 domain-like	H: Sigma2 domain of RNA polymerase sigma factors (From Topology)	T: Sigma2 domain of RNA polymerase sigma factors	F: Sigma70_r2	ECOD (1.6)
B	Cupin_7	e2z2sB1	A: beta sandwiches	X: jelly-roll	H: Double-stranded beta-helix (From Topology)	T: Double-stranded beta-helix	F: Cupin_7	ECOD (1.6)
B	zf-HC2	e2z2sB3	A: alpha arrays	X: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	H: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	T: N-terminal, cytoplasmic domain of anti-sigma factors	F: zf-HC2	ECOD (1.6)
D	Cupin_7	e2z2sD2	A: beta sandwiches	X: jelly-roll	H: Double-stranded beta-helix (From Topology)	T: Double-stranded beta-helix	F: Cupin_7	ECOD (1.6)
D	zf-HC2	e2z2sD3	A: alpha arrays	X: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	H: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	T: N-terminal, cytoplasmic domain of anti-sigma factors	F: zf-HC2	ECOD (1.6)
F	Cupin_7	e2z2sF2	A: beta sandwiches	X: jelly-roll	H: Double-stranded beta-helix (From Topology)	T: Double-stranded beta-helix	F: Cupin_7	ECOD (1.6)
F	zf-HC2	e2z2sF3	A: alpha arrays	X: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	H: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	T: N-terminal, cytoplasmic domain of anti-sigma factors	F: zf-HC2	ECOD (1.6)
H	Cupin_7	e2z2sH2	A: beta sandwiches	X: jelly-roll	H: Double-stranded beta-helix (From Topology)	T: Double-stranded beta-helix	F: Cupin_7	ECOD (1.6)
H	zf-HC2	e2z2sH3	A: alpha arrays	X: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	H: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	T: N-terminal, cytoplasmic domain of anti-sigma factors	F: zf-HC2	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B	1.10.10.1320	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Anti-sigma factor, zinc-finger domain	CATH (4.3.0)
B	2.60.120.10	Mainly Beta	Sandwich	Jelly Rolls	Jelly Rolls	CATH (4.3.0)
D	1.10.10.1320	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Anti-sigma factor, zinc-finger domain	CATH (4.3.0)
D	2.60.120.10	Mainly Beta	Sandwich	Jelly Rolls	Jelly Rolls	CATH (4.3.0)
F	1.10.10.1320	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Anti-sigma factor, zinc-finger domain	CATH (4.3.0)
F	2.60.120.10	Mainly Beta	Sandwich	Jelly Rolls	Jelly Rolls	CATH (4.3.0)
H	1.10.10.1320	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Anti-sigma factor, zinc-finger domain	CATH (4.3.0)
H	2.60.120.10	Mainly Beta	Sandwich	Jelly Rolls	Jelly Rolls	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, C, E, G	PF04542	Sigma-70 region 2 (Sigma70_r2)	Sigma-70 region 2	Region 2 of sigma-70 is the most conserved region of the entire protein. All members of this class of sigma-factor contain region 2. The high conservation is due to region 2 containing both the -10 promoter recognition helix and the primary core RNA ...	Region 2 of sigma-70 is the most conserved region of the entire protein. All members of this class of sigma-factor contain region 2. The high conservation is due to region 2 containing both the -10 promoter recognition helix and the primary core RNA polymerase binding determinant. The core binding helix, interacts with the clamp domain of the largest polymerase subunit, beta prime [1,2]. The aromatic residues of the recognition helix, found at the C-terminus of this domain are though to mediate strand separation, thereby allowing transcription initiation [1,2].	Domain
A, C, E, G	PF04545	Sigma-70, region 4 (Sigma70_r4)	Sigma-70, region 4	Region 4 of sigma-70 like sigma-factors are involved in binding to the -35 promoter element via a helix-turn-helix motif [1]. Due to the way Pfam works, the threshold has been set artificially high to prevent overlaps with other helix-turn-helix fami ...	Region 4 of sigma-70 like sigma-factors are involved in binding to the -35 promoter element via a helix-turn-helix motif [1]. Due to the way Pfam works, the threshold has been set artificially high to prevent overlaps with other helix-turn-helix families. Therefore there are many false negatives.	Domain
B, D, F, H	PF12973	ChrR Cupin-like domain (Cupin_7)	ChrR Cupin-like domain	Members of this family are part of the cupin superfamily. This family includes the transcriptional activator ChrR Swiss:P40685.	Domain
B, D, F, H	PF13490	Putative zinc-finger (zf-HC2)	Putative zinc-finger	This is a putative zinc-finger found in some anti-sigma factor proteins.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, C, E, G	RpoE, ECF SigE	DNA binding binding nucleic acid binding transcription regulator activity DNA-binding transcription factor activity sigma factor activity	RNA metabolic process primary metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process DNA-templated transcription initiation nucleic acid metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process biosynthetic process metabolic process cellular process RNA metabolic process primary metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process DNA-templated transcription initiation nucleic acid metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process biosynthetic process metabolic process cellular process macromolecule metabolic process	-
B, D, F, H	Anti-Sigma factor ChrR, transcriptional activator ChrR	DNA binding binding nucleic acid binding cation binding ion binding metal ion binding small molecule binding	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, C, E, G	IPR014284	RNA polymerase sigma-70-like domain	Domain
A, C, E, G	IPR007627	RNA polymerase sigma-70 region 2	Domain
A, C, E, G	IPR039425	RNA polymerase sigma-70-like	Family
A, C, E, G	IPR013325	RNA polymerase sigma factor, region 2	Homologous Superfamily
A, C, E, G	IPR013324	RNA polymerase sigma factor, region 3/4-like	Homologous Superfamily
A, C, E, G	IPR036388	Winged helix-like DNA-binding domain superfamily	Homologous Superfamily
A, C, E, G	IPR007630	RNA polymerase sigma-70 region 4	Domain
B, D, F, H	IPR041916	Anti-sigma factor, zinc-finger domain superfamily	Homologous Superfamily
B, D, F, H	IPR025979	ChrR-like cupin domain	Domain
B, D, F, H	IPR011051	RmlC-like cupin domain superfamily	Homologous Superfamily
B, D, F, H	IPR027383	Putative zinc-finger	Domain
B, D, F, H	IPR012807	Anti-sigma-E factor ChrR	Family
B, D, F, H	IPR014710	RmlC-like jelly roll fold	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.