2VFX

Structure of the Symmetric Mad2 Dimer

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
H	d2vfxh_	Alpha and beta proteins (a+b)	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	automated matches	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
L	d2vfxl_	Alpha and beta proteins (a+b)	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	automated matches	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
A	d2vfxa_	Alpha and beta proteins (a+b)	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	automated matches	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d2vfxb_	Alpha and beta proteins (a+b)	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	automated matches	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
C	d2vfxc_	Alpha and beta proteins (a+b)	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	automated matches	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
D	d2vfxd_	Alpha and beta proteins (a+b)	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	automated matches	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
E	d2vfxe_	Alpha and beta proteins (a+b)	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	automated matches	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
F	d2vfxf_	Alpha and beta proteins (a+b)	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	automated matches	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
G	d2vfxg_	Alpha and beta proteins (a+b)	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	automated matches	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
I	d2vfxi_	Alpha and beta proteins (a+b)	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	automated matches	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
J	d2vfxj_	Alpha and beta proteins (a+b)	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	automated matches	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
K	d2vfxk_	Alpha and beta proteins (a+b)	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	The spindle assembly checkpoint protein mad2	automated matches	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
H	SCOP2B Superfamily	HORMA/p31comet-like	8004003	3000150	SCOP2B (2022-06-29)
L	SCOP2B Superfamily	HORMA/p31comet-like	8004003	3000150	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	HORMA/p31comet-like	8004003	3000150	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	HORMA/p31comet-like	8004003	3000150	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	HORMA/p31comet-like	8004003	3000150	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	HORMA/p31comet-like	8004003	3000150	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	HORMA/p31comet-like	8004003	3000150	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	HORMA/p31comet-like	8004003	3000150	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	HORMA/p31comet-like	8004003	3000150	SCOP2B (2022-06-29)
I	SCOP2B Superfamily	HORMA/p31comet-like	8004003	3000150	SCOP2B (2022-06-29)
J	SCOP2B Superfamily	HORMA/p31comet-like	8004003	3000150	SCOP2B (2022-06-29)
K	SCOP2B Superfamily	HORMA/p31comet-like	8004003	3000150	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
H	HORMA	e2vfxH1	A: a+b two layers	X: The spindle assembly checkpoint protein mad2 (From Topology)	H: The spindle assembly checkpoint protein mad2 (From Topology)	T: The spindle assembly checkpoint protein mad2	F: HORMA	ECOD (1.6)
L	HORMA	e2vfxL1	A: a+b two layers	X: The spindle assembly checkpoint protein mad2 (From Topology)	H: The spindle assembly checkpoint protein mad2 (From Topology)	T: The spindle assembly checkpoint protein mad2	F: HORMA	ECOD (1.6)
A	HORMA	e2vfxA1	A: a+b two layers	X: The spindle assembly checkpoint protein mad2 (From Topology)	H: The spindle assembly checkpoint protein mad2 (From Topology)	T: The spindle assembly checkpoint protein mad2	F: HORMA	ECOD (1.6)
B	HORMA	e2vfxB1	A: a+b two layers	X: The spindle assembly checkpoint protein mad2 (From Topology)	H: The spindle assembly checkpoint protein mad2 (From Topology)	T: The spindle assembly checkpoint protein mad2	F: HORMA	ECOD (1.6)
C	HORMA	e2vfxC1	A: a+b two layers	X: The spindle assembly checkpoint protein mad2 (From Topology)	H: The spindle assembly checkpoint protein mad2 (From Topology)	T: The spindle assembly checkpoint protein mad2	F: HORMA	ECOD (1.6)
D	HORMA	e2vfxD1	A: a+b two layers	X: The spindle assembly checkpoint protein mad2 (From Topology)	H: The spindle assembly checkpoint protein mad2 (From Topology)	T: The spindle assembly checkpoint protein mad2	F: HORMA	ECOD (1.6)
E	HORMA	e2vfxE1	A: a+b two layers	X: The spindle assembly checkpoint protein mad2 (From Topology)	H: The spindle assembly checkpoint protein mad2 (From Topology)	T: The spindle assembly checkpoint protein mad2	F: HORMA	ECOD (1.6)
F	HORMA	e2vfxF1	A: a+b two layers	X: The spindle assembly checkpoint protein mad2 (From Topology)	H: The spindle assembly checkpoint protein mad2 (From Topology)	T: The spindle assembly checkpoint protein mad2	F: HORMA	ECOD (1.6)
G	HORMA	e2vfxG1	A: a+b two layers	X: The spindle assembly checkpoint protein mad2 (From Topology)	H: The spindle assembly checkpoint protein mad2 (From Topology)	T: The spindle assembly checkpoint protein mad2	F: HORMA	ECOD (1.6)
I	HORMA	e2vfxI1	A: a+b two layers	X: The spindle assembly checkpoint protein mad2 (From Topology)	H: The spindle assembly checkpoint protein mad2 (From Topology)	T: The spindle assembly checkpoint protein mad2	F: HORMA	ECOD (1.6)
J	HORMA	e2vfxJ1	A: a+b two layers	X: The spindle assembly checkpoint protein mad2 (From Topology)	H: The spindle assembly checkpoint protein mad2 (From Topology)	T: The spindle assembly checkpoint protein mad2	F: HORMA	ECOD (1.6)
K	HORMA	e2vfxK1	A: a+b two layers	X: The spindle assembly checkpoint protein mad2 (From Topology)	H: The spindle assembly checkpoint protein mad2 (From Topology)	T: The spindle assembly checkpoint protein mad2	F: HORMA	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
H	3.30.900.10	Alpha Beta	2-Layer Sandwich	Cell Cycle, Spindle Assembly Checkpoint Protein	Chain A	CATH (4.3.0)
L	3.30.900.10	Alpha Beta	2-Layer Sandwich	Cell Cycle, Spindle Assembly Checkpoint Protein	Chain A	CATH (4.3.0)
A	3.30.900.10	Alpha Beta	2-Layer Sandwich	Cell Cycle, Spindle Assembly Checkpoint Protein	Chain A	CATH (4.3.0)
B	3.30.900.10	Alpha Beta	2-Layer Sandwich	Cell Cycle, Spindle Assembly Checkpoint Protein	Chain A	CATH (4.3.0)
C	3.30.900.10	Alpha Beta	2-Layer Sandwich	Cell Cycle, Spindle Assembly Checkpoint Protein	Chain A	CATH (4.3.0)
D	3.30.900.10	Alpha Beta	2-Layer Sandwich	Cell Cycle, Spindle Assembly Checkpoint Protein	Chain A	CATH (4.3.0)
E	3.30.900.10	Alpha Beta	2-Layer Sandwich	Cell Cycle, Spindle Assembly Checkpoint Protein	Chain A	CATH (4.3.0)
F	3.30.900.10	Alpha Beta	2-Layer Sandwich	Cell Cycle, Spindle Assembly Checkpoint Protein	Chain A	CATH (4.3.0)
G	3.30.900.10	Alpha Beta	2-Layer Sandwich	Cell Cycle, Spindle Assembly Checkpoint Protein	Chain A	CATH (4.3.0)
I	3.30.900.10	Alpha Beta	2-Layer Sandwich	Cell Cycle, Spindle Assembly Checkpoint Protein	Chain A	CATH (4.3.0)
J	3.30.900.10	Alpha Beta	2-Layer Sandwich	Cell Cycle, Spindle Assembly Checkpoint Protein	Chain A	CATH (4.3.0)
K	3.30.900.10	Alpha Beta	2-Layer Sandwich	Cell Cycle, Spindle Assembly Checkpoint Protein	Chain A	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	PF02301	HORMA domain (HORMA)	HORMA domain	The HORMA (for Hop1p, Rev7p and MAD2) domain has been suggested to recognise chromatin states that result from DNA adducts, double stranded breaks or non-attachment to the spindle and acts as an adaptor that recruits other proteins. MAD2 is a spindle ...	The HORMA (for Hop1p, Rev7p and MAD2) domain has been suggested to recognise chromatin states that result from DNA adducts, double stranded breaks or non-attachment to the spindle and acts as an adaptor that recruits other proteins. MAD2 is a spindle checkpoint protein which prevents progression of the cell cycle upon detection of a defect in mitotic spindle integrity.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2A	identical protein binding protein binding binding protein homodimerization activity protein dimerization activity	cellular process cell division regulation of biological process regulation of cellular process negative regulation of programmed cell death regulation of apoptotic process biological regulation negative regulation of biological process regulation of programmed cell death cell death negative regulation of apoptotic process negative regulation of cellular process apoptotic process programmed cell death cellular process cell division regulation of biological process regulation of cellular process negative regulation of programmed cell death regulation of apoptotic process biological regulation negative regulation of biological process regulation of programmed cell death cell death negative regulation of apoptotic process negative regulation of cellular process apoptotic process programmed cell death cellular response to stimulus positive regulation of cell communication regulation of cell cycle phase transition positive regulation of signal transduction response to stimulus positive regulation of cell cycle checkpoint spindle checkpoint signaling negative regulation of cell cycle regulation of signal transduction regulation of mitotic nuclear division intracellular signal transduction negative regulation of mitotic cell cycle cell communication regulation of cell cycle checkpoint negative regulation of mitotic sister chromatid segregation negative regulation of chromosome organization negative regulation of cell cycle phase transition mitotic spindle checkpoint signaling signal transduction cell cycle mitotic sister chromatid separation chromosome segregation negative regulation of sister chromatid segregation mitotic spindle assembly checkpoint signaling negative regulation of cellular component organization regulation of cell cycle process regulation of spindle checkpoint sister chromatid segregation positive regulation of intracellular signal transduction regulation of chromosome organization regulation of organelle organization regulation of mitotic cell cycle phase transition regulation of mitotic sister chromatid segregation mitotic cell cycle process regulation of response to stimulus positive regulation of mitotic cell cycle spindle assembly checkpoint regulation of chromosome separation negative regulation of mitotic nuclear division regulation of chromosome segregation regulation of cellular component organization regulation of mitotic sister chromatid separation regulation of metaphase/anaphase transition of cell cycle regulation of mitotic spindle checkpoint signaling regulation of mitotic cell cycle spindle assembly checkpoint spindle assembly checkpoint signaling positive regulation of biological process cell cycle phase transition regulation of mitotic metaphase/anaphase transition mitotic cell cycle cellular component organization positive regulation of response to stimulus regulation of mitotic cell cycle regulation of intracellular signal transduction mitotic sister chromatid segregation positive regulation of cell cycle process negative regulation of mitotic metaphase/anaphase transition cellular component organization or biogenesis regulation of cell cycle negative regulation of chromosome separation positive regulation of cellular process cell cycle process positive regulation of spindle checkpoint mitotic nuclear division regulation of signaling negative regulation of mitotic cell cycle phase transition organelle fission chromosome organization negative regulation of cell cycle process positive regulation of cell cycle cell cycle checkpoint signaling mitotic cell cycle checkpoint signaling organelle organization nuclear division negative regulation of chromosome segregation chromosome separation negative regulation of organelle organization negative regulation of nuclear division regulation of sister chromatid segregation negative regulation of mitotic sister chromatid separation regulation of nuclear division negative regulation of metaphase/anaphase transition of cell cycle nuclear chromosome segregation metaphase/anaphase transition of mitotic cell cycle regulation of cell communication metaphase/anaphase transition of cell cycle mitotic cell cycle phase transition positive regulation of signaling establishment of organelle localization cellular localization microtubule cytoskeleton organization spindle localization establishment or maintenance of cell polarity localization establishment of mitotic spindle localization establishment of spindle localization establishment of spindle orientation organelle localization establishment of localization in cell microtubule cytoskeleton organization involved in mitosis establishment of localization establishment of cell polarity establishment of mitotic spindle orientation microtubule-based process cytoskeleton organization catabolic process macromolecule catabolic process negative regulation of protein catabolic process regulation of primary metabolic process protein metabolic process negative regulation of protein metabolic process negative regulation of macromolecule metabolic process negative regulation of catabolic process regulation of protein metabolic process negative regulation of metabolic process primary metabolic process protein catabolic process regulation of macromolecule metabolic process regulation of catabolic process metabolic process macromolecule metabolic process regulation of metabolic process regulation of protein catabolic process negative regulation of post-translational protein modification negative regulation of ubiquitin protein ligase activity regulation of protein modification process regulation of catalytic activity regulation of protein ubiquitination negative regulation of catalytic activity regulation of transferase activity regulation of ubiquitin protein ligase activity negative regulation of ubiquitin-protein transferase activity protein ubiquitination negative regulation of transferase activity negative regulation of protein modification process regulation of ubiquitin-protein transferase activity negative regulation of protein ubiquitination regulation of protein modification by small protein conjugation or removal protein modification process negative regulation of protein modification by small protein conjugation or removal regulation of post-translational protein modification negative regulation of molecular function regulation of molecular function macromolecule modification post-translational protein modification protein modification by small protein conjugation protein modification by small protein conjugation or removal centrosome localization microtubule organizing center localization establishment of centrosome localization	intracellular organelle cellular anatomical structure intracellular anatomical structure cytoskeleton spindle organelle membraneless organelle intracellular membraneless organelle spindle pole microtubule cytoskeleton cytoplasm cytosol protein-containing complex mitotic spindle assembly checkpoint MAD1-MAD2 complex intracellular organelle cellular anatomical structure intracellular anatomical structure cytoskeleton spindle organelle membraneless organelle intracellular membraneless organelle spindle pole microtubule cytoskeleton cytoplasm cytosol protein-containing complex mitotic spindle assembly checkpoint MAD1-MAD2 complex mitotic spindle membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen nuclear protein-containing complex endomembrane system organelle envelope nuclear envelope nuclear pore nuclear basket nuclear pore chromosome chromosomal region kinetochore chromosome, centromeric region condensed chromosome, centromeric region supramolecular complex condensed chromosome perinuclear region of cytoplasm mitotic checkpoint complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR045091	Mad2-like	Family
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR036570	HORMA domain superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR003511	HORMA domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	Pharos: Q13257	polycystic ovary syndrome esophageal squamous cell carcinoma malignant pancreatic neoplasm non-small cell lung carcinoma pancreatic intraductal papillary-mucinous neoplasm primitive neuroectodermal tumor adrenal cortex carcinoma Sjogren syndrome breast cancer ductal breast carcinoma in situ glioblastoma IgA glomerulonephritis lung adenocarcinoma malignant ependymoma medulloblastoma polycystic ovary syndrome esophageal squamous cell carcinoma malignant pancreatic neoplasm non-small cell lung carcinoma pancreatic intraductal papillary-mucinous neoplasm primitive neuroectodermal tumor adrenal cortex carcinoma Sjogren syndrome breast cancer ductal breast carcinoma in situ glioblastoma IgA glomerulonephritis lung adenocarcinoma malignant ependymoma medulloblastoma ovarian cancer pituitary cancer psoriasis atopic eczema breast carcinoma endometriosis heart disease invasive ductal breast carcinoma lung cancer malignant glioma nasopharyngeal carcinoma pancreatic intraductal papillary-mucinous carcinoma Sclerocystic Ovaries atypical teratoid rhabdoid tumor Crohn disease hepatocellular carcinoma large cell medulloblastoma malignant colon neoplasm

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.