2LZ6 | pdb_00002lz6

Distinct ubiquitin binding modes exhibited by sh3 domains: molecular determinants and functional implications

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2lz6a_	Alpha and beta proteins (a+b)	beta-Grasp (ubiquitin-like)	Ubiquitin-like	Ubiquitin-related	Ubiquitin	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d2lz6b_	All beta proteins	SH3-like barrel	SH3-domain	automated matches	automated matches	mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Ubiquitin-like	8043829	3000157	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	ubiquitin_1	e2lz6A1	A: a+b two layers	X: beta-Grasp	H: Ubiquitin-related	T: Ubiquitin-like	F: ubiquitin_1	ECOD (1.6)
B	SH3_1	e2lz6B1	A: beta barrels	X: SH3	H: SH3	T: SH3	F: SH3_1	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.10.20.90	Alpha Beta	Roll	Ubiquitin-like (UB roll)	Phosphatidylinositol 3-kinase Catalytic Subunit	CATH (4.3.0)
B	2.30.30.40	Mainly Beta	Roll	SH3 type barrels.	SH3 Domains	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00240	Ubiquitin family (ubiquitin)	Ubiquitin family	This family contains a number of ubiquitin-like proteins: SUMO (smt3 homologue) (see Swiss:Q02724), Nedd8 (see Swiss:P29595), Elongin B (see Swiss:Q15370), Rub1 (see Swiss:Q9SHE7), and Parkin (see Swiss:O60260). A number of them are thought to carry ...	This family contains a number of ubiquitin-like proteins: SUMO (smt3 homologue) (see Swiss:Q02724), Nedd8 (see Swiss:P29595), Elongin B (see Swiss:Q15370), Rub1 (see Swiss:Q9SHE7), and Parkin (see Swiss:O60260). A number of them are thought to carry a distinctive five-residue motif termed the proteasome-interacting motif (PIM), which may have a biologically significant role in protein delivery to proteasomes and recruitment of proteasomes to transcription sites [5].	Domain
B	PF00018	SH3 domain (SH3_1)	SH3 domain	SH3 (Src homology 3) domains are often indicative of a protein involved in signal transduction related to cytoskeletal organisation. First described in the Src cytoplasmic tyrosine kinase Swiss:P12931. The structure is a partly opened beta barrel.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Ubiquitin	protein tag activity molecular tag activity ubiquitin protein ligase binding protein binding binding ubiquitin-like protein ligase binding enzyme binding RNA binding nucleic acid binding	catabolic process proteolysis involved in protein catabolic process primary metabolic process protein catabolic process macromolecule catabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis metabolic process cellular process macromolecule metabolic process protein metabolic process protein ubiquitination macromolecule modification catabolic process proteolysis involved in protein catabolic process primary metabolic process protein catabolic process macromolecule catabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis metabolic process cellular process macromolecule metabolic process protein metabolic process protein ubiquitination macromolecule modification post-translational protein modification protein modification process protein modification by small protein conjugation protein modification by small protein conjugation or removal	membrane-bounded organelle intracellular anatomical structure membrane intracellular vesicle intracellular membrane-bounded organelle intracellular organelle cellular anatomical structure cytoplasmic vesicle membrane bounding membrane of organelle cytoplasmic vesicle vesicle membrane organelle endocytic vesicle membrane cytoplasm membrane-bounded organelle intracellular anatomical structure membrane intracellular vesicle intracellular membrane-bounded organelle intracellular organelle cellular anatomical structure cytoplasmic vesicle membrane bounding membrane of organelle cytoplasmic vesicle vesicle membrane organelle endocytic vesicle membrane cytoplasm vesicle organelle membrane endocytic vesicle extracellular exosome extracellular vesicle extracellular organelle extracellular region extracellular space extracellular membrane-bounded organelle cytosol endosome membrane endosome endomembrane system mitochondrial membrane mitochondrion organelle outer membrane organelle envelope mitochondrial envelope mitochondrial outer membrane outer membrane cell periphery plasma membrane nucleus membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen organelle subcompartment endoplasmic reticulum subcompartment nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum endoplasmic reticulum membrane
B	CD2-associated protein	cytoskeletal protein binding actin binding protein-containing complex binding protein binding binding actin filament binding SH3 domain binding protein domain specific binding identical protein binding clathrin binding phosphatidylinositol 3-kinase binding phosphatidylinositol 3-kinase regulatory subunit binding	developmental process multicellular organismal process gonad development reproductive system development reproductive process sex differentiation animal organ development developmental process involved in reproduction male gonad development male sex differentiation multicellular organism development development of primary male sexual characteristics development of primary sexual characteristics anatomical structure development reproductive structure development developmental process multicellular organismal process gonad development reproductive system development reproductive process sex differentiation animal organ development developmental process involved in reproduction male gonad development male sex differentiation multicellular organism development development of primary male sexual characteristics development of primary sexual characteristics anatomical structure development reproductive structure development system development D-glucose import hexose transmembrane transport transport carbohydrate transport establishment of localization transmembrane transport localization D-glucose transmembrane transport cellular process monosaccharide transmembrane transport carbohydrate transmembrane transport immune system development lymph node development immune system process hematopoietic or lymphoid organ development cell division cell population proliferation renal filtration cell differentiation kidney epithelium development cellular developmental process glomerular epithelial cell differentiation epithelial cell differentiation renal system development cell differentiation nephron development nephron epithelium development epithelial cell differentiation involved in kidney development epithelium development glomerular epithelium development tissue development glomerulus development cell differentiation involved in kidney development podocyte differentiation kidney development apoptotic process cell death programmed cell death catabolic process metabolic process primary metabolic process protein catabolic process protein metabolic process macromolecule metabolic process macromolecule catabolic process cellular component organization cellular component biogenesis cellular component assembly protein-containing complex organization protein complex oligomerization protein-containing complex assembly cellular component organization or biogenesis protein heterooligomerization cellular response to stimulus regulation of biological process small GTPase-mediated signal transduction regulation of cellular process response to stimulus signaling biological regulation intracellular signal transduction cell communication intracellular signaling cassette Rab protein signal transduction signal transduction renal system process system process renal absorption renal albumin absorption renal protein absorption localization of cell endothelium development response to stress response to wounding protein localization establishment of protein localization cellular localization nitrogen compound transport secretion establishment of protein localization to extracellular region export from cell protein secretion protein localization to extracellular region secretion by cell protein transport cellular macromolecule localization macromolecule localization organelle organization actin cytoskeleton organization actin filament organization actin filament-based process supramolecular fiber organization cytoskeleton organization plasma membrane bounded cell projection organization cell projection organization cell development neuron projection development nervous system development generation of neurons neurogenesis neuron differentiation neuron development hepaticobiliary system development gland development liver development cellular response to growth factor stimulus cell surface receptor signaling pathway response to endogenous stimulus cellular response to nerve growth factor stimulus cellular response to endogenous stimulus nerve growth factor signaling pathway neurotrophin signaling pathway response to nerve growth factor response to growth factor regulation of localization regulation of protein localization positive regulation of protein localization to nucleus positive regulation of biological process regulation of protein localization to nucleus protein localization to nucleus regulation of cellular localization protein localization to organelle positive regulation of protein localization positive regulation of cellular process connective tissue development adipose tissue development immune response-regulating cell surface receptor signaling pathway positive regulation of response to stimulus immune response-activating signaling pathway immune response-regulating signaling pathway positive regulation of immune response regulation of immune system process positive regulation of immune system process immune response-activating cell surface receptor signaling pathway immune response T cell receptor signaling pathway regulation of response to stimulus regulation of immune response antigen receptor-mediated signaling pathway activation of immune response phosphatidylinositol 3-kinase/protein kinase B signal transduction stress-activated MAPK cascade stress-activated protein kinase signaling cascade cellular response to stress MAPK cascade transforming growth factor beta1 production biosynthetic process cytokine production gene expression macromolecule biosynthetic process transforming growth factor beta production ERK1 and ERK2 cascade regulation of cytoskeleton organization regulation of cellular component organization regulation of actin filament-based process regulation of organelle organization regulation of actin cytoskeleton organization response to transforming growth factor beta negative regulation of signaling regulation of small GTPase mediated signal transduction negative regulation of intracellular signal transduction negative regulation of biological process regulation of signal transduction negative regulation of cell communication regulation of intracellular signal transduction negative regulation of cellular process regulation of signaling negative regulation of response to stimulus negative regulation of small GTPase mediated signal transduction negative regulation of signal transduction regulation of cell communication protein polymerization actin polymerization or depolymerization actin filament polymerization lipid metabolic process cell junction organization synapse organization regulation of transforming growth factor beta production regulation of transforming growth factor beta1 production regulation of cytokine production regulation of multicellular organismal process negative regulation of macromolecule metabolic process regulation of biosynthetic process negative regulation of transforming growth factor beta1 production negative regulation of metabolic process negative regulation of cytokine production regulation of macromolecule metabolic process negative regulation of transforming growth factor beta production regulation of gene expression negative regulation of gene expression negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process regulation of macromolecule biosynthetic process negative regulation of multicellular organismal process regulation of metabolic process neuron apoptotic process negative regulation of neuron apoptotic process negative regulation of programmed cell death regulation of apoptotic process regulation of programmed cell death negative regulation of apoptotic process regulation of neuron apoptotic process cell adhesion cell-cell adhesion cell-cell adhesion mediated by cadherin axonogenesis developmental growth plasma membrane bounded cell projection morphogenesis cell growth cell morphogenesis cell projection morphogenesis neuron projection morphogenesis collateral sprouting developmental cell growth growth axon development cell morphogenesis involved in neuron differentiation developmental growth involved in morphogenesis anatomical structure morphogenesis positive regulation of establishment of protein localization positive regulation of secretion by cell positive regulation of protein transport regulation of secretion by cell positive regulation of secretion regulation of transport regulation of establishment of protein localization regulation of protein transport positive regulation of protein secretion regulation of protein secretion regulation of secretion positive regulation of transport filopodium assembly plasma membrane bounded cell projection assembly cell projection assembly response to hormone response to chemical response to peptide hormone response to insulin response to oxygen-containing compound response to nitrogen compound regulation of synaptic plasticity trans-synaptic signaling modulation of chemical synaptic transmission cell-cell signaling chemical synaptic transmission synaptic signaling regulation of trans-synaptic signaling anterograde trans-synaptic signaling regulation of biological quality membrane organization neurotrophin TRK receptor signaling pathway cell surface receptor protein tyrosine kinase signaling pathway enzyme-linked receptor protein signaling pathway immunological synapse formation cell-cell recognition cell activation cell recognition lymphocyte activation leukocyte activation maintenance of blood-brain barrier multicellular organismal-level homeostasis homeostatic process anatomical structure homeostasis tissue homeostasis biological process involved in interspecies interaction between organisms response to other organism response to external stimulus response to external biotic stimulus response to virus response to biotic stimulus defense response inflammatory response response to oxidative stress response to glial cell derived neurotrophic factor cell-cell junction organization	cell-cell junction cellular anatomical structure anchoring junction cell junction intracellular organelle intracellular anatomical structure podosome cytoskeleton organelle membraneless organelle intracellular membraneless organelle actin cytoskeleton cytoplasm cytosol cell-cell junction cellular anatomical structure anchoring junction cell junction intracellular organelle intracellular anatomical structure podosome cytoskeleton organelle membraneless organelle intracellular membraneless organelle actin cytoskeleton cytoplasm cytosol somatodendritic compartment cell projection dendrite dendritic tree neuron projection plasma membrane bounded cell projection neuromuscular junction synapse membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle endomembrane system intracellular organelle lumen organelle envelope lumen organelle envelope organelle lumen nuclear envelope lumen nuclear envelope ruffle cell leading edge polymeric cytoskeletal fiber actin filament supramolecular fiber supramolecular polymer supramolecular complex protein-containing complex filamentous actin axon cell periphery cell cortex vesicle centrosome microtubule organizing center centriolar satellite microtubule cytoskeleton filtration diaphragm slit diaphragm plasma membrane membrane growth cone distal axon site of polarized growth Golgi apparatus subcompartment Golgi apparatus organelle subcompartment trans-Golgi network membrane trans-Golgi network organelle membrane nucleolus fibrillar center nuclear lumen endosome intracellular vesicle cytoplasmic vesicle late endosome

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR019954	Ubiquitin conserved site	Conserved Site
A	IPR000626	Ubiquitin-like domain	Domain
A	IPR019956	Ubiquitin domain	Domain
A	IPR050158	Ubiquitin and ubiquitin-like	Family
A	IPR029071	Ubiquitin-like domain superfamily	Homologous Superfamily
B	IPR036028	SH3-like domain superfamily	Homologous Superfamily
B	IPR035777	CD2-associated protein, third SH3 domain	Domain
B	IPR001452	SH3 domain	Domain
B	IPR035775	CD2-associated protein, first SH3 domain	Domain
B	IPR050384	Endophilin/SH3RF	Family
B	IPR035776	CD2-associated protein, second SH3 domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: P0CG48	acute myeloid leukemia heart disease myocardial infarction pancreatic ductal adenocarcinoma astrocytic tumor lung cancer osteosarcoma renal cell carcinoma glioblastoma malignant pancreatic neoplasm ovarian cancer

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.