2H6I

W102T/Y365F Protein Farnesyltransferase Double Mutant Complexed with a Geranylgeranylated DDPTASACVLS Peptide Product at 3.0A

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2h6ia1	All alpha proteins	alpha-alpha superhelix	Protein prenylyltransferase	Protein prenylyltransferase	Protein farnesyltransferase alpha-subunit	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d2h6ib1	All alpha proteins	alpha/alpha toroid	Terpenoid cyclases/Protein prenyltransferases	Protein prenyltransferases	Protein farnesyltransferase, beta-subunit	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Protein prenylyltransferase	8042173	3001312	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Terpenoid cyclases/Protein prenyltransferases	8042174	3001024	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PPTA	e2h6iA1	A: alpha superhelices	X: Repetitive alpha hairpins	H: ARM repeat (From Topology)	T: ARM repeat	F: PPTA	ECOD (1.6)
B	SQHop_cyclase_C	e2h6iB1	A: alpha superhelices	X: Repetitive alpha hairpins	H: alpha/alpha toroid (From Topology)	T: alpha/alpha toroid	F: SQHop_cyclase_C	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.25.40.120	Mainly Alpha	Alpha Horseshoe	Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat	Protein prenylyltransferase	CATH (4.3.0)
B	1.50.10.20	Mainly Alpha	Alpha/alpha barrel	Glycosyltransferase		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF01239	Protein prenyltransferase alpha subunit repeat (PPTA)	Protein prenyltransferase alpha subunit repeat	-	Repeat
B	PF00432	Prenyltransferase alpha-alpha toroid domain (Prenyltrans)	Prenyltransferase and squalene oxidase repeat	-	Repeat

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Protein farnesyltransferase/geranylgeranyltransferase type I alpha subunit	cytoskeletal protein binding protein binding binding tubulin binding alpha-tubulin binding microtubule binding catalytic activity, acting on a protein protein geranylgeranyltransferase activity protein prenyltransferase activity Rab geranylgeranyltransferase activity prenyltransferase activity transferase activity catalytic activity transferase activity, transferring alkyl or aryl (other than methyl) groups cytoskeletal protein binding protein binding binding tubulin binding alpha-tubulin binding microtubule binding catalytic activity, acting on a protein protein geranylgeranyltransferase activity protein prenyltransferase activity Rab geranylgeranyltransferase activity prenyltransferase activity transferase activity catalytic activity transferase activity, transferring alkyl or aryl (other than methyl) groups molecular adaptor activity protein farnesyltransferase activity protein tyrosine kinase binding kinase binding signaling receptor binding receptor tyrosine kinase binding enzyme binding protein kinase binding CAAX-protein geranylgeranyltransferase activity	cellular response to stimulus positive regulation of cell communication small GTPase-mediated signal transduction regulation of cellular process positive regulation of signal transduction response to stimulus signaling regulation of small GTPase mediated signal transduction positive regulation of biological process regulation of signal transduction intracellular signal transduction cell communication positive regulation of response to stimulus regulation of intracellular signal transduction intracellular signaling cassette cellular response to stimulus positive regulation of cell communication small GTPase-mediated signal transduction regulation of cellular process positive regulation of signal transduction response to stimulus signaling regulation of small GTPase mediated signal transduction positive regulation of biological process regulation of signal transduction intracellular signal transduction cell communication positive regulation of response to stimulus regulation of intracellular signal transduction intracellular signaling cassette signal transduction positive regulation of cellular process regulation of biological process regulation of signaling Rac protein signal transduction biological regulation positive regulation of intracellular signal transduction regulation of Rac protein signal transduction positive regulation of Rac protein signal transduction regulation of response to stimulus regulation of cell communication cellular process positive regulation of small GTPase mediated signal transduction positive regulation of signaling protein localization regulation of localization cellular localization regulation of protein localization cellular component organization localization neuromuscular junction development receptor clustering cellular macromolecule localization synapse organization cellular component organization or biogenesis regulation of skeletal muscle acetylcholine-gated channel clustering regulation of receptor clustering postsynaptic membrane organization regulation of protein localization to membrane membrane organization postsynapse organization positive regulation of receptor clustering skeletal muscle acetylcholine-gated channel clustering positive regulation of protein localization to membrane localization within membrane protein localization to membrane positive regulation of skeletal muscle acetylcholine-gated channel clustering cell junction organization regulation of cellular localization macromolecule localization positive regulation of protein localization positive regulation of molecular function regulation of molecular function positive regulation of deacetylase activity regulation of catalytic activity regulation of deacetylase activity positive regulation of catalytic activity regulation of protein modification process positive regulation of protein deacetylation positive regulation of protein metabolic process macromolecule deacylation positive regulation of tubulin deacetylation protein deacetylation regulation of primary metabolic process positive regulation of metabolic process positive regulation of protein modification process regulation of protein deacetylation tubulin deacetylation positive regulation of macromolecule metabolic process protein metabolic process protein modification process regulation of protein metabolic process primary metabolic process regulation of macromolecule metabolic process protein deacylation regulation of tubulin deacetylation macromolecule modification metabolic process macromolecule metabolic process regulation of metabolic process protein farnesylation protein prenylation prenylation protein geranylgeranylation cellular response to growth factor stimulus cell surface receptor protein serine/threonine kinase signaling pathway cell surface receptor signaling pathway cellular response to transforming growth factor beta stimulus response to endogenous stimulus cellular response to endogenous stimulus response to transforming growth factor beta transforming growth factor beta receptor signaling pathway response to growth factor transforming growth factor beta receptor superfamily signaling pathway enzyme-linked receptor protein signaling pathway	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol cell periphery plasma membrane membrane protein-containing complex protein farnesyltransferase complex catalytic complex polyprenyl diphosphate synthase complex transferase complex intracellular organelle cytoskeleton cellular anatomical structure intracellular anatomical structure cytoplasm cytosol cell periphery plasma membrane membrane protein-containing complex protein farnesyltransferase complex catalytic complex polyprenyl diphosphate synthase complex transferase complex intracellular organelle cytoskeleton organelle membraneless organelle microtubule associated complex intracellular membraneless organelle microtubule cytoskeleton CAAX-protein geranylgeranyltransferase complex intracellular protein-containing complex
B	Protein farnesyltransferase beta subunit	protein farnesyltransferase activity catalytic activity, acting on a protein protein prenyltransferase activity prenyltransferase activity transferase activity catalytic activity transferase activity, transferring alkyl or aryl (other than methyl) groups cation binding transition metal ion binding zinc ion binding ion binding binding metal ion binding small molecule binding	primary metabolic process metabolic process cellular process lipid metabolic process protein farnesylation macromolecule modification protein prenylation protein metabolic process macromolecule metabolic process prenylation protein modification process	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol protein-containing complex protein farnesyltransferase complex catalytic complex polyprenyl diphosphate synthase complex transferase complex intracellular organelle cytoskeleton organelle membraneless organelle microtubule associated complex cellular anatomical structure intracellular anatomical structure cytoplasm cytosol protein-containing complex protein farnesyltransferase complex catalytic complex polyprenyl diphosphate synthase complex transferase complex intracellular organelle cytoskeleton organelle membraneless organelle microtubule associated complex intracellular membraneless organelle microtubule cytoskeleton
C [auth P]	farnesylated peptide	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR002088	Protein prenyltransferase, alpha subunit	Repeat
B	IPR045089	Prenyltransferase subunit beta	Family
B	IPR026872	Protein farnesyltransferase subunit beta	Family
B	IPR008930	Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid	Homologous Superfamily
B	IPR001330	Prenyltransferase alpha-alpha toroid domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: P49354	acute myeloid leukemia Hutchinson-Gilford progeria syndrome ovarian cancer diabetes mellitus lymphoplasmacytic lymphoma pituitary cancer Down syndrome osteosarcoma plasma cell myeloma
B	Pharos: P49356	Hutchinson-Gilford progeria syndrome

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.