2FMX

An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg(2+)

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2fmxa_	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	G proteins	automated matches	Chinese hamster (Cricetulus griseus ) [TaxId: 10029 ],	SCOPe (2.08)
B	d2fmxb_	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	G proteins	automated matches	Chinese hamster (Cricetulus griseus ) [TaxId: 10029 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Ras-like P-loop GTPases	8060269	3002022	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Ras-like P-loop GTPases	8060269	3002022	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Ras	e2fmxA1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Ras	ECOD (1.6)
B	Ras	e2fmxB1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Ras	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
B	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	GTP-binding protein SAR1b	binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding organic cyclic compound binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding organic cyclic compound binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding amino acid sensor activity molecular sensor activity molecular function regulator activity pyrophosphatase activity GTPase activity G protein activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity cation binding metal ion binding	establishment of organelle localization vesicle budding from membrane cellular localization regulation of cellular component organization regulation of cellular process transport vesicle targeting, to, from or within Golgi vesicle-mediated transport vesicle targeting cellular component organization cellular component biogenesis vesicle organization localization regulation of protein-containing complex assembly cellular component assembly establishment of organelle localization vesicle budding from membrane cellular localization regulation of cellular component organization regulation of cellular process transport vesicle targeting, to, from or within Golgi vesicle-mediated transport vesicle targeting cellular component organization cellular component biogenesis vesicle organization localization regulation of protein-containing complex assembly cellular component assembly organelle localization establishment of localization in cell protein-containing complex assembly vesicle coating regulation of COPII vesicle coating cellular component organization or biogenesis vesicle targeting, rough ER to cis-Golgi membrane organization vesicle localization regulation of biological process protein-containing complex organization establishment of vesicle localization biological regulation intracellular transport endoplasmic reticulum to Golgi vesicle-mediated transport regulation of cellular component biogenesis establishment of localization regulation of organelle organization organelle organization cellular process COPII-coated vesicle budding COPII vesicle coating Golgi vesicle transport chemical homeostasis homeostatic process lipid homeostasis lipid export from cell macromolecule localization lipid localization export from cell lipid transport cellular response to stimulus response to nutrient levels response to stimulus cellular response to leucine starvation cellular response to amino acid starvation cellular response to starvation cellular response to nutrient levels cellular response to stress response to stress response to amino acid starvation response to starvation protein localization regulation of protein exit from endoplasmic reticulum regulation of localization protein exit from endoplasmic reticulum establishment of protein localization nitrogen compound transport regulation of protein localization positive regulation of biological process positive regulation of establishment of protein localization positive regulation of protein transport cellular macromolecule localization positive regulation of intracellular transport positive regulation of cellular process regulation of intracellular transport regulation of transport regulation of establishment of protein localization regulation of protein transport positive regulation of protein exit from endoplasmic reticulum positive regulation of transport protein transport regulation of cellular localization positive regulation of protein localization regulation of intracellular protein transport positive regulation of intracellular protein transport intracellular protein transport regulation of lipid transport regulation of lipid localization lipoprotein transport lipoprotein localization signaling negative regulation of signaling negative regulation of intracellular signal transduction negative regulation of biological process regulation of signal transduction intracellular signal transduction negative regulation of cell communication cell communication negative regulation of TORC1 signaling regulation of intracellular signal transduction negative regulation of TOR signaling negative regulation of cellular process signal transduction regulation of TORC1 signaling TOR signaling regulation of signaling regulation of TOR signaling negative regulation of response to stimulus negative regulation of signal transduction regulation of response to stimulus regulation of cell communication TORC1 signaling vesicle cargo loading COPII-coated vesicle cargo loading	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle Golgi cisterna membrane Golgi apparatus subcompartment Golgi apparatus organelle subcompartment membrane Golgi cisterna intracellular membrane-bounded organelle endomembrane system intracellular organelle cellular anatomical structure intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle Golgi cisterna membrane Golgi apparatus subcompartment Golgi apparatus organelle subcompartment membrane Golgi cisterna intracellular membrane-bounded organelle endomembrane system intracellular organelle organelle Golgi stack organelle membrane protein-containing complex vesicle coat intracellular vesicle transport vesicle membrane membrane protein complex membrane coat bounding membrane of organelle vesicle membrane cytoplasmic vesicle ER to Golgi transport vesicle membrane coated vesicle vesicle transport vesicle coated membrane COPII vesicle coat cytoplasmic vesicle membrane coated vesicle membrane COPII-coated ER to Golgi transport vesicle endoplasmic reticulum subcompartment nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum endoplasmic reticulum membrane endoplasmic reticulum exit site vacuolar membrane lytic vacuole vacuole lytic vacuole membrane lysosomal membrane lysosome

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
A, B	IPR005225	Small GTP-binding domain	Domain
A, B	IPR006687	Small GTPase superfamily, SAR1-type	Family
A, B	IPR006689	Small GTPase superfamily, ARF/SAR type	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.