1XXF

Crystal Structure of the FXIa Catalytic Domain in Complex with Ecotin Mutant (EcotinP)

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1xxfa_	All beta proteins	Trypsin-like serine proteases	Trypsin-like serine proteases	Eukaryotic proteases	Coagulation factor XI	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d1xxfb_	All beta proteins	Trypsin-like serine proteases	Trypsin-like serine proteases	Eukaryotic proteases	Coagulation factor XI	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
C	d1xxfc_	All beta proteins	Ecotin, trypsin inhibitor	Ecotin, trypsin inhibitor	Ecotin, trypsin inhibitor	Ecotin, trypsin inhibitor	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
D	d1xxfd_	All beta proteins	Ecotin, trypsin inhibitor	Ecotin, trypsin inhibitor	Ecotin, trypsin inhibitor	Ecotin, trypsin inhibitor	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Trypsin-like serine proteases	8044201	3000114	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Trypsin-like serine proteases	8044201	3000114	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Ecotin, trypsin inhibitor	8041127	3001256	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Ecotin, trypsin inhibitor	8041127	3001256	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Trypsin_1	e1xxfA1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: FMN-binding split barrel 2	F: Trypsin_1	ECOD (1.6)
B	Trypsin_1	e1xxfB1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: FMN-binding split barrel 2	F: Trypsin_1	ECOD (1.6)
C	Ecotin	e1xxfC1	A: beta sandwiches	X: Ecotin, trypsin inhibitor (From Topology)	H: Ecotin, trypsin inhibitor (From Topology)	T: Ecotin, trypsin inhibitor	F: Ecotin	ECOD (1.6)
D	Ecotin	e1xxfD1	A: beta sandwiches	X: Ecotin, trypsin inhibitor (From Topology)	H: Ecotin, trypsin inhibitor (From Topology)	T: Ecotin, trypsin inhibitor	F: Ecotin	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.40.10.10	Mainly Beta	Beta Barrel	Thrombin, subunit H	Trypsin-like serine proteases	CATH (4.3.0)
B	2.40.10.10	Mainly Beta	Beta Barrel	Thrombin, subunit H	Trypsin-like serine proteases	CATH (4.3.0)
C	2.60.40.550	Mainly Beta	Sandwich	Immunoglobulin-like	Ecotin	CATH (4.3.0)
D	2.60.40.550	Mainly Beta	Sandwich	Immunoglobulin-like	Ecotin	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF00089	Trypsin (Trypsin)	Trypsin	-	Domain
C, D	PF03974	Ecotin (Ecotin)	Ecotin	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Coagulation factor XI	serine-type exopeptidase activity peptidase activity serine-type aminopeptidase activity catalytic activity, acting on a protein serine hydrolase activity serine-type peptidase activity aminopeptidase activity hydrolase activity exopeptidase activity catalytic activity serine-type endopeptidase activity endopeptidase activity heparin binding glycosaminoglycan binding serine-type exopeptidase activity peptidase activity serine-type aminopeptidase activity catalytic activity, acting on a protein serine hydrolase activity serine-type peptidase activity aminopeptidase activity hydrolase activity exopeptidase activity catalytic activity serine-type endopeptidase activity endopeptidase activity heparin binding glycosaminoglycan binding binding carbohydrate derivative binding sulfur compound binding identical protein binding protein binding	multicellular organismal process regulation of body fluid levels wound healing response to stimulus hemostasis biological regulation blood coagulation response to stress coagulation regulation of biological quality response to wounding regulation of response to external stimulus negative regulation of biological process positive regulation of biological process multicellular organismal process regulation of body fluid levels wound healing response to stimulus hemostasis biological regulation blood coagulation response to stress coagulation regulation of biological quality response to wounding regulation of response to external stimulus negative regulation of biological process positive regulation of biological process regulation of coagulation regulation of response to wounding regulation of response to stress regulation of multicellular organismal process regulation of hemostasis regulation of biological process negative regulation of response to stimulus regulation of blood coagulation negative regulation of wound healing negative regulation of blood coagulation response to external stimulus negative regulation of response to wounding positive regulation of fibrinolysis negative regulation of hemostasis fibrinolysis regulation of fibrinolysis negative regulation of coagulation regulation of response to stimulus negative regulation of response to external stimulus regulation of wound healing negative regulation of multicellular organismal process zymogen activation primary metabolic process gene expression macromolecule biosynthetic process protein maturation biosynthetic process proteolysis protein processing metabolic process cellular process protein metabolic process macromolecule metabolic process plasminogen activation	cellular anatomical structure membrane extracellular region extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle organelle extracellular space vesicle extracellular membrane-bounded organelle cell periphery plasma membrane
C, D	Ecotin	enzyme regulator activity molecular function inhibitor activity peptidase regulator activity serine-type endopeptidase inhibitor activity molecular function regulator activity enzyme inhibitor activity peptidase inhibitor activity endopeptidase regulator activity endopeptidase inhibitor activity identical protein binding protein binding binding protein homodimerization activity protein dimerization activity	response to stimulus defense response response to stress negative regulation of hydrolase activity negative regulation of proteolysis regulation of cellular process regulation of catalytic activity negative regulation of biological process negative regulation of catalytic activity regulation of primary metabolic process negative regulation of peptidase activity regulation of proteolysis proteolysis negative regulation of cellular process response to stimulus defense response response to stress negative regulation of hydrolase activity negative regulation of proteolysis regulation of cellular process regulation of catalytic activity negative regulation of biological process negative regulation of catalytic activity regulation of primary metabolic process negative regulation of peptidase activity regulation of proteolysis proteolysis negative regulation of cellular process protein metabolic process negative regulation of protein metabolic process negative regulation of macromolecule metabolic process regulation of biological process regulation of protein metabolic process negative regulation of metabolic process biological regulation negative regulation of molecular function regulation of peptidase activity primary metabolic process regulation of macromolecule metabolic process regulation of molecular function regulation of hydrolase activity metabolic process cellular process macromolecule metabolic process regulation of metabolic process	cellular anatomical structure outer membrane-bounded periplasmic space cell envelope periplasmic space

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR000177	Apple domain	Domain
A, B	IPR009003	Peptidase S1, PA clan	Homologous Superfamily
A, B	IPR001314	Peptidase S1A, chymotrypsin family	Family
A, B	IPR033116	Serine proteases, trypsin family, serine active site	Active Site
A, B	IPR001254	Serine proteases, trypsin domain	Domain
A, B	IPR003609	PAN/Apple domain	Domain
A, B	IPR043504	Peptidase S1, PA clan, chymotrypsin-like fold	Homologous Superfamily
A, B	IPR018114	Serine proteases, trypsin family, histidine active site	Active Site
C, D	IPR005658	Proteinase inhibitor I11, ecotin	Family
C, D	IPR036198	Ecotin superfamily	Homologous Superfamily
C, D	IPR023084	Proteinase inhibitor I11, ecotin, gammaproteobacteria	Family
C, D	IPR027438	Ecotin, C-terminal	Homologous Superfamily

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B	Pharos: P03951	congenital factor XI deficiency hypertensive disorder cerebrovascular disorder lung carcinoma pancreatic ductal adenocarcinoma pancreatic intraductal papillary-mucinous neoplasm with low grade dysplasia lung adenocarcinoma malignant pancreatic neoplasm pancreatic intraductal papillary-mucinous carcinoma thrombotic disease factor XI deficiency lung cancer non-small cell lung carcinoma pancreatic intraductal papillary-mucinous neoplasm vascular disease

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.