1BDF | pdb_00001bdf

STRUCTURE OF ESCHERICHIA COLI RNA POLYMERASE ALPHA SUBUNIT N-TERMINAL DOMAIN

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1bdfa1	Alpha and beta proteins (a+b)	DCoH-like	RBP11-like subunits of RNA polymerase	RNA polymerase alpha subunit dimerisation domain	RNA polymerase alpha	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
A	d1bdfa2	Alpha and beta proteins (a+b)	Insert subdomain of RNA polymerase alpha subunit	Insert subdomain of RNA polymerase alpha subunit	Insert subdomain of RNA polymerase alpha subunit	RNA polymerase alpha subunit	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
B	d1bdfb1	Alpha and beta proteins (a+b)	DCoH-like	RBP11-like subunits of RNA polymerase	RNA polymerase alpha subunit dimerisation domain	RNA polymerase alpha	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
B	d1bdfb2	Alpha and beta proteins (a+b)	Insert subdomain of RNA polymerase alpha subunit	Insert subdomain of RNA polymerase alpha subunit	Insert subdomain of RNA polymerase alpha subunit	RNA polymerase alpha subunit	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
C	d1bdfc1	Alpha and beta proteins (a+b)	DCoH-like	RBP11-like subunits of RNA polymerase	RNA polymerase alpha subunit dimerisation domain	RNA polymerase alpha	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
C	d1bdfc2	Alpha and beta proteins (a+b)	Insert subdomain of RNA polymerase alpha subunit	Insert subdomain of RNA polymerase alpha subunit	Insert subdomain of RNA polymerase alpha subunit	RNA polymerase alpha subunit	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
D	d1bdfd1	Alpha and beta proteins (a+b)	DCoH-like	RBP11-like subunits of RNA polymerase	RNA polymerase alpha subunit dimerisation domain	RNA polymerase alpha	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
D	d1bdfd2	Alpha and beta proteins (a+b)	Insert subdomain of RNA polymerase alpha subunit	Insert subdomain of RNA polymerase alpha subunit	Insert subdomain of RNA polymerase alpha subunit	RNA polymerase alpha subunit	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	RNA polymerase alpha subunit dimerisation domain	8023527	4001475	SCOP2 (2022-06-29)
A	SCOP2 Family	Insert subdomain of RNA polymerase alpha subunit	8024900	4002623	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	RBP11-like subunits of RNA polymerase	8035907	3001655	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Insert subdomain of RNA polymerase alpha subunit	8037279	3001500	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	RBP11-like subunits of RNA polymerase	8035907	3001655	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Insert subdomain of RNA polymerase alpha subunit	8037279	3001500	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Insert subdomain of RNA polymerase alpha subunit	8037279	3001500	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	RBP11-like subunits of RNA polymerase	8035907	3001655	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Insert subdomain of RNA polymerase alpha subunit	8037279	3001500	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	RBP11-like subunits of RNA polymerase	8035907	3001655	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	RNA_pol_L	e1bdfA1	A: a+b two layers	X: DCoH-like	H: RBP11-like subunits of RNA polymerase (From Topology)	T: RBP11-like subunits of RNA polymerase	F: RNA_pol_L	ECOD (1.6)
A	RNA_pol_A_bac	e1bdfA2	A: a+b complex topology	X: Ribosomal protein TL5/L25 C-terminal domain-like	H: Insert subdomain of RNA polymerase alpha subunit (From Topology)	T: Insert subdomain of RNA polymerase alpha subunit	F: RNA_pol_A_bac	ECOD (1.6)
B	RNA_pol_L	e1bdfB1	A: a+b two layers	X: DCoH-like	H: RBP11-like subunits of RNA polymerase (From Topology)	T: RBP11-like subunits of RNA polymerase	F: RNA_pol_L	ECOD (1.6)
B	RNA_pol_A_bac	e1bdfB2	A: a+b complex topology	X: Ribosomal protein TL5/L25 C-terminal domain-like	H: Insert subdomain of RNA polymerase alpha subunit (From Topology)	T: Insert subdomain of RNA polymerase alpha subunit	F: RNA_pol_A_bac	ECOD (1.6)
C	RNA_pol_L	e1bdfC1	A: a+b two layers	X: DCoH-like	H: RBP11-like subunits of RNA polymerase (From Topology)	T: RBP11-like subunits of RNA polymerase	F: RNA_pol_L	ECOD (1.6)
C	RNA_pol_A_bac	e1bdfC2	A: a+b complex topology	X: Ribosomal protein TL5/L25 C-terminal domain-like	H: Insert subdomain of RNA polymerase alpha subunit (From Topology)	T: Insert subdomain of RNA polymerase alpha subunit	F: RNA_pol_A_bac	ECOD (1.6)
D	RNA_pol_L	e1bdfD1	A: a+b two layers	X: DCoH-like	H: RBP11-like subunits of RNA polymerase (From Topology)	T: RBP11-like subunits of RNA polymerase	F: RNA_pol_L	ECOD (1.6)
D	RNA_pol_A_bac	e1bdfD2	A: a+b complex topology	X: Ribosomal protein TL5/L25 C-terminal domain-like	H: Insert subdomain of RNA polymerase alpha subunit (From Topology)	T: Insert subdomain of RNA polymerase alpha subunit	F: RNA_pol_A_bac	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.1360.10	Alpha Beta	2-Layer Sandwich	Gyrase A	domain 2	CATH (4.3.0)
A	2.170.120.12	Mainly Beta	Beta Complex	RNA Polymerase Alpha Subunit	Chain A, domain 2	CATH (4.3.0)
B	3.30.1360.10	Alpha Beta	2-Layer Sandwich	Gyrase A	domain 2	CATH (4.3.0)
B	2.170.120.12	Mainly Beta	Beta Complex	RNA Polymerase Alpha Subunit	Chain A, domain 2	CATH (4.3.0)
C	3.30.1360.10	Alpha Beta	2-Layer Sandwich	Gyrase A	domain 2	CATH (4.3.0)
C	2.170.120.12	Mainly Beta	Beta Complex	RNA Polymerase Alpha Subunit	Chain A, domain 2	CATH (4.3.0)
D	3.30.1360.10	Alpha Beta	2-Layer Sandwich	Gyrase A	domain 2	CATH (4.3.0)
D	2.170.120.12	Mainly Beta	Beta Complex	RNA Polymerase Alpha Subunit	Chain A, domain 2	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF01193	RNA polymerase Rpb3/Rpb11 dimerisation domain (RNA_pol_L)	RNA polymerase Rpb3/Rpb11 dimerisation domain	The two eukaryotic subunits Rpb3 and Rpb11 dimerise to from a platform onto which the other subunits of the RNA polymerase assemble (D/L in archaea). The prokaryotic equivalent of the Rpb3/Rpb11 platform is the alpha-alpha dimer. The dimerisation do ...	The two eukaryotic subunits Rpb3 and Rpb11 dimerise to from a platform onto which the other subunits of the RNA polymerase assemble (D/L in archaea). The prokaryotic equivalent of the Rpb3/Rpb11 platform is the alpha-alpha dimer. The dimerisation domain of the alpha subunit/Rpb3 is interrupted by an insert domain (Pfam:PF01000). Some of the alpha subunits also contain iron-sulphur binding domains (Pfam:PF00037). Rpb11 is found as a continuous domain. Members of this family include: alpha subunit from eubacteria, alpha subunits from chloroplasts, Rpb3 subunits from eukaryotes, Rpb11 subunits from eukaryotes, RpoD subunits from archaeal spp, and RpoL subunits from archaeal spp.	Domain
A, B, C, D	PF01000	RNA polymerase Rpb3/RpoA insert domain (RNA_pol_A_bac)	RNA polymerase Rpb3/RpoA insert domain	Members of this family include: alpha subunit from eubacteria alpha subunits from chloroplasts Rpb3 subunits from eukaryotes RpoD subunits from archaeal	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	RNA POLYMERASE ALPHA SUBUNIT	DNA binding binding nucleic acid binding protein binding protein dimerization activity	intracellular iron ion homeostasis inorganic ion homeostasis cellular homeostasis chemical homeostasis homeostatic process intracellular monoatomic cation homeostasis monoatomic ion homeostasis intracellular chemical homeostasis monoatomic cation homeostasis intracellular monoatomic ion homeostasis negative regulation of termination of DNA-templated transcription regulation of cellular process negative regulation of biological process protein-containing complex disassembly intracellular iron ion homeostasis inorganic ion homeostasis cellular homeostasis chemical homeostasis homeostatic process intracellular monoatomic cation homeostasis monoatomic ion homeostasis intracellular chemical homeostasis monoatomic cation homeostasis intracellular monoatomic ion homeostasis negative regulation of termination of DNA-templated transcription regulation of cellular process negative regulation of biological process protein-containing complex disassembly positive regulation of macromolecule biosynthetic process regulation of protein-containing complex disassembly regulation of primary metabolic process negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process positive regulation of metabolic process DNA-templated transcription regulation of RNA metabolic process positive regulation of macromolecule metabolic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process cellular component disassembly negative regulation of DNA-templated transcription regulation of biological process negative regulation of cellular component organization negative regulation of metabolic process primary metabolic process regulation of gene expression biosynthetic process negative regulation of macromolecule biosynthetic process regulation of termination of DNA-templated transcription regulation of macromolecule biosynthetic process metabolic process macromolecule metabolic process regulation of DNA-templated transcription regulation of metabolic process negative regulation of RNA metabolic process regulation of cellular component organization transcription antitermination positive regulation of biological process RNA metabolic process DNA-templated transcription termination RNA biosynthetic process cellular component organization nucleic acid biosynthetic process positive regulation of biosynthetic process negative regulation of protein-containing complex disassembly negative regulation of cellular process cellular component organization or biogenesis positive regulation of cellular process regulation of RNA biosynthetic process negative regulation of macromolecule metabolic process regulation of biosynthetic process protein-containing complex organization biological regulation regulation of macromolecule metabolic process gene expression nucleobase-containing compound biosynthetic process macromolecule biosynthetic process nucleic acid metabolic process positive regulation of gene expression negative regulation of biosynthetic process cellular process regulation of DNA-templated transcription initiation DNA-templated transcription initiation cellular response to stimulus cellular response to stress response to stimulus response to stress cellular response to cell envelope stress organelle assembly cell projection organization bacterial-type flagellum assembly membraneless organelle assembly cellular component biogenesis bacterial-type flagellum organization organelle organization cellular component assembly cell projection assembly nitrate assimilation ammonium ion metabolic process nitrogen cycle metabolic process oxoacid metabolic process small molecule metabolic process organic acid metabolic process nitrate metabolic process reactive nitrogen species metabolic process regulation of DNA-templated transcription elongation DNA-templated transcription elongation cell aggregation biofilm formation aggregation of unicellular organisms submerged biofilm formation cell motility response to heat response to abiotic stimulus response to temperature stimulus bacterial-type flagellum-dependent cell motility archaeal or bacterial-type flagellum-dependent cell motility cilium or flagellum-dependent cell motility	protein-containing complex intracellular anatomical structure catalytic complex transferase complex transferase complex, transferring phosphorus-containing groups cellular anatomical structure cytosolic DNA-directed RNA polymerase complex RNA polymerase complex DNA-directed RNA polymerase complex cytoplasm cytosol intracellular protein-containing complex membrane membrane-bounded organelle protein-containing complex intracellular anatomical structure catalytic complex transferase complex transferase complex, transferring phosphorus-containing groups cellular anatomical structure cytosolic DNA-directed RNA polymerase complex RNA polymerase complex DNA-directed RNA polymerase complex cytoplasm cytosol intracellular protein-containing complex membrane membrane-bounded organelle transcription elongation factor complex nucleus membrane-enclosed lumen nuclear protein-containing complex intracellular membrane-bounded organelle intracellular organelle lumen intracellular organelle nucleoplasm organelle lumen organelle nuclear lumen

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR011773	DNA-directed RNA polymerase, alpha subunit	Family
A, B, C, D	IPR011260	RNA polymerase, alpha subunit, C-terminal	Domain
A, B, C, D	IPR011263	DNA-directed RNA polymerase, RpoA/D/Rpb3-type	Domain
A, B, C, D	IPR036603	RNA polymerase, RBP11-like subunit	Homologous Superfamily
A, B, C, D	IPR011262	DNA-directed RNA polymerase, insert domain	Domain
A, B, C, D	IPR036643	DNA-directed RNA polymerase, insert domain superfamily	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.